Animal hide and skin identification method

Abstract

The invention discloses an animal hide and skin identification method, and particularly discloses a method for identifying donkey hide by means of liquid chromatography-mass spectrometry technology. The method can be used for identifying and distinguishing donkey hide, horsehide, mule hide and cowhide. The method comprises the steps of 1, test solution preparation, 2, liquid chromatography-mass spectrometer detection and 3, animal hide sample judgment according to a mass spectrum. The method is used for identifying and distinguishing donkey hide, horsehide, mule hide and cowhide, the identification accuracy is 100%, and the method has the advantages of being high in specificity, good in reproducibility, easy to operate and the like.

Description

Technical field [0001] The invention relates to a method for identifying animal skins, in particular to a method for identifying donkey skins using liquid mass spectrometry technology, which can be used for identifying and distinguishing donkey skins, horse skins, mule skins and cowhides. Background technique [0002] Ejiao is a traditional Chinese patent medicine with thousands of years of edible history. It has the effects of nourishing blood, nourishing yin, moisturizing dryness, and stopping bleeding. According to the Chinese Pharmacopoeia, donkey-hide gelatin is a solid glue made from the dried skin or fresh skin of the equine donkey (Equusasinus L.) by decoction and concentration. Pure donkey-hide gelatin must come from 100% donkey skin to ensure the effectiveness of the product. [0003] At present, when purchasing donkey skins, it is necessary to identify donkey skins to prevent the use of horse skins, mule skins, and cowhide as donkey skins. The method of identifying the...

AI Technical Summary

Problems solved by technology

The method of identifying the authenticity of donkey hides by direct observation often has misjudgment or ambiguity, which is subjective and cannot be accurately distinguished. It is even more difficult to distinguish between horse hides, mule hides and donkey hides that are similar in shape.

Although the DNA molecular identification method can distinguish donkey hide from horse hide, it is a little weak to distinguish donkey hide from mule hide, and the operation is more cumbersome, there are false negatives and false positives, the cost of detection consumables is relatively expensive, and the time required Also longer, it is difficult to achieve batch detection

[0004] The detection method of LC-MS technology is very mature. As long as the characteristic components that are stable in animal-derived components are found, they can be used for detection; Skin-derived components, turtle-derived components, deer-derived components, etc. Although there are reports on the detection methods of donkey skin-derived components, they are actually common components of donkey and horse, and it is impossible to distinguish donkey skin from horse skin

At present, there is still no detection method for distinguishing donkeys, horses, and mules by using liquid chromatography-mass spectrometry. very close, and their differences in biological nature are very limited

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