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Method for preparing doramectin sterile solution

A doramectin and solution technology, applied in the field of preparation of doramectin sterile solution, can solve the problems of long production cycle, high production cost, weak market competitiveness, etc. The effect of the production cycle

Active Publication Date: 2016-01-13
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1 The extraction yield is low
The average extraction yield is only about 70%
[0005] 2. The doramectin fermentation liquid was not pretreated, and the moisture content in the wet mushroom residue exceeded 60%. Organic solvents were directly used for extraction, resulting in an increase in the volume of the solution and increasing the processing difficulty of downstream processes.
[0006] 3 The production process is relatively complicated and the production cycle is long
[0007] 4 The production cost is high, compared with similar products, the market competitiveness is weak

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Doramectin fermentation broth 10m 3 , titer is 1324mg / L.

[0037] Fermentation broth pretreatment: First, adjust the pH of the fermentation broth to 2 with 20% hydrochloric acid solution, stir for 10 minutes, and obtain wet fungus residue after filtration. Then, add 2m of purified water to the wet fungus residue 3 , stirred for 10 min, and filtered again. Finally, add purified water 4m 3 , adjust the pH to 9 with 30% ammonia solution, stir for 20 minutes, and then let stand for 80 minutes. After standing still, the fermented liquid is subjected to plate and frame filtration to obtain the doramectin wet fungus residue.

[0038] Flash drying: Use a flash dryer, control the inlet air temperature at 100-120°C, the outlet air temperature at 70-90°C, and the feeding speed at 50-400r / min. After flash drying, 756 kg of dried fungus residue of doramectin was obtained, wherein the moisture content was 4.1%.

[0039] Organic solvent A leaching: for the first leaching, add 30...

Embodiment 2

[0048] Doramectin fermentation 10m 3 , titer is 1296mg / L.

[0049] Fermentation broth pretreatment: First, adjust the pH of the fermentation broth to 2.3 with 23% sulfuric acid solution, stir for 13 minutes, and obtain wet fungus residue after filtration. Secondly, add 2.3m of purified water to the wet fungus residue 3 , stirred for 13 minutes, and filtered again; finally, 4.2m of purified water was added to the wet fungus residue obtained by filtering again 3 , adjust the pH of the doramectin fermentation broth to 9.3 with 33% sodium hydroxide solution, stir for 25 minutes, and then let stand for 85 minutes. After standing still, the fermented liquid is subjected to plate and frame filtration to obtain the doramectin wet fungus residue.

[0050] Flash drying: Use a flash dryer, control the inlet air temperature at 100-120°C, the outlet air temperature at 70-90°C, and the feeding speed at 50-400r / min. After flash drying, 731kg of dried fungus residue of doramectin was obta...

Embodiment 3

[0060] Doramectin fermentation 10m 3 , titer is 1247mg / L.

[0061] Fermentation broth pretreatment: First, adjust the pH of the fermentation broth to 2.5 with 25% hydrochloric acid solution, stir for 15 minutes, and obtain wet fungus residue after filtration. Secondly, add 2.5m of purified water to the wet fungus residue 3 , stirred for 15 minutes, and filtered again; finally, 4.5m of purified water was added to the wet fungus residue obtained by filtering again 3 , adjust the pH of the doramectin fermentation broth to 9.5 with 35% ammonia solution, stir for 30 minutes, and then let stand for 90 minutes. After standing still, the fermented liquid is subjected to plate and frame filtration to obtain the doramectin wet fungus residue.

[0062] Flash drying: Use a flash dryer, control the inlet air temperature at 100-120°C, the outlet air temperature at 70-90°C, and the feeding speed at 50-400r / min. After flash drying, 747kg of dried fungus residue of doramectin was obtained,...

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PUM

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Abstract

The invention relates to a method for preparing a doramectin sterile solution. The method comprises the following steps: firstly, pre-treating a doramectin fermentation broth prepared by fermenting mutational streptomyces avermitilis which serves as a strain; performing flash drying to obtain dried strain slag, and extracting with an organic solvent a; cooling and adding purified water to separate; filtering to obtain a doramectin crude product, and dissolving the crude product with an organic solvent; performing ultrafiltration and crystallization; and dissolving a solid doramectin obtained by one more filtration with an organic solvent B; and performing microfiltration to obtain the doramectin sterile solution. The method can be used for realizing effective separation and purification on the doramectin in the fermentation broth, the total extraction yield is over 85 percent, the product quality accords with the requirement, the production cost is reduced, and the international market competition of doramectin can be improved.

Description

technical field [0001] The invention belongs to the technical field of antibiotic extraction, in particular to a method for preparing a doramectin sterile solution. Background technique [0002] Doramectin is an abamectin antibiotic fermented by a new strain of genetically recombined Streptomyces avermitilis by adding cyclohexamethylene carboxylic acid as a precursor substance, and belongs to the third generation of Avermectin drugs. [0003] In the prior art, doramycin is extracted by using organic alcohol extraction combined with ion exchange technology or organic alcohol extraction combined with chromatography technology to extract and purify doramectin fermentation liquid to obtain its finished product. The main problems are: [0004] 1 The extraction yield is low. The average extraction yield is only about 70%. [0005] 2. The doramectin fermentation liquid was not pretreated, and the moisture content in the wet mushroom residue exceeded 60%. Organic solvents were dir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K31/7048
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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