Thermosensitive dual-administration nanocomposite hydrogel as well as preparation method and application thereof

A nano-composite, temperature-sensitive technology, applied in the field of biomedical materials and medical devices, can solve the limitations of deep wound repair and other problems, and achieve the effects of inhibiting bacterial growth, strong operability, and simple preparation methods

Inactive Publication Date: 2016-01-13
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some hydrogel varieties have been developed in the market at present, such as chitosan, hyaluronic acid, alginic acid, collagen hydrogel, etc., they lack the function of controlling the release of drugs and growth factors, and have a great role in deep wound repair. limitations

Method used

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  • Thermosensitive dual-administration nanocomposite hydrogel as well as preparation method and application thereof
  • Thermosensitive dual-administration nanocomposite hydrogel as well as preparation method and application thereof
  • Thermosensitive dual-administration nanocomposite hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Preparation of poly(N-isopropylacrylamide) nanospheres and loading of growth factors

[0056] ①Dissolve 6mmol of N-isopropylacrylamide in 100mL of ionized water, then add 0.3mmol of N-N'methylenebisacrylamide and 0.15mmol of sodium dodecylsulfonate respectively, and heat up to 70°C, add 0.2mmol ammonium persulfate, stir and react for 7h, then dialyze with deionized water for 7d after cooling, and freeze-dry for 48h to obtain poly(N-isopropylacrylamide) nanospheres with a particle size of 115nm;

[0057] ② Disperse 60 mg of poly(N-isopropylacrylamide) nanospheres prepared in step (1) ① into 5 mL of dilute hydrochloric acid aqueous solution at pH=4.0 to make a suspension, and dissolve 15 mg of β-transforming growth factor in 5 mL of pH =4.0 in dilute hydrochloric acid aqueous solution;

[0058] ③ Mix and stir the suspension of nano-microspheres prepared in step (1)② and the dilute hydrochloric acid aqueous solution of the growth factor, carry out the loading of the g...

Embodiment 2

[0066] (1) Preparation of poly(N-isopropylacrylamide) nanospheres and loading of growth factors

[0067] ①Dissolve 6mmol of N-isopropylacrylamide in 100mL of ionized water, then add 0.06mmol of N-N'methylenebisacrylamide and 0.06mmol of sodium dodecylsulfonate respectively, and raise the temperature to 60°C, add 0.06mmol ammonium persulfate, stir and react for 4 hours, then dialyze with deionized water for 7 days after cooling, and freeze-dry for 48 hours to obtain poly(N-isopropylacrylamide) nanospheres with a particle size of 280nm;

[0068] ② Disperse 50 mg of poly(N-isopropylacrylamide) nanospheres prepared in step (1) ① into 5 mL of dilute hydrochloric acid aqueous solution at pH=4.0 to make a suspension, and dissolve 5 mg of α-transforming growth factor in 5 mL of pH =5.0 in dilute hydrochloric acid aqueous solution;

[0069] ③ Mix and stir the nano-microsphere suspension prepared in step (1)② and the dilute aqueous solution of growth factor hydrochloric acid, carry out...

Embodiment 3

[0076] (1) Preparation of poly(N-isopropylacrylamide) nanospheres and loading of growth factors

[0077] ①Dissolve 6mmol of N-isopropylacrylamide in 100mL of ionized water, then add 0.6mmol of N-N'methylenebisacrylamide and 0.3mmol of sodium dodecylsulfonate respectively, and raise the temperature to At 80°C, add 0.3mmol ammonium persulfate, stir and react for 24 hours, then dialyze with deionized water for 7 days after cooling, and freeze-dry for 48 hours to obtain poly(N-isopropylacrylamide) nanospheres with a particle size of 50nm;

[0078] ② Disperse 100 mg of poly(N-isopropylacrylamide) nanospheres prepared in step (1) ① into 5 mL of dilute hydrochloric acid aqueous solution at pH=4.0 to make a suspension, and dissolve 25 mg of platelet-derived growth factor in 5 mL of pH =3.0 in dilute hydrochloric acid aqueous solution;

[0079] ③Mix and stir the nano-microsphere suspension prepared in step (1)② and the dilute hydrochloric acid aqueous solution of the growth factor, ca...

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Abstract

The invention belongs to the technical fields of biomedical materials and medical apparatus, and in particular relates to a thermosensitive dual-administration nanocomposite hydrogel as well as a preparation method and application thereof. The preparation method of the thermosensitive dual-administration nanocomposite hydrogel comprises the following steps: preparing a poly (N-isopropylacrylamide) nano microsphere and loading a growth factor, preparing a poly (N-isopropylacrylamide-co-acrylic acid) nano microsphere and loading an anti-inflammatory drug, and preparing the thermosensitive dual-administration nanocomposite hydrogel. The finished product takes sodium alginate as a matrix phase and uses two poly (N-isopropylacrylamide) based thermosensitive nano gel microspheres which have different LCSTs and are respectively loaded with the anti-inflammatory drug and the growth factor as a dispersion phase; and by controlling the temperature of the composite hydrogel to be higher than or lower than LCST of the nano microspheres, the release of the anti-inflammatory drug and the release of the growth factor are controlled, so that controlled release of the anti-inflammatory drug and the growth factor in inflammatory stage and proliferative stage of a deep wound repairing process is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and medical devices, and in particular relates to a temperature-sensitive dual-administration nanocomposite hydrogel and its preparation method and application. Background technique [0002] Skin tissue is prone to defects or loss of function due to burns, mechanical trauma, and chronic ulcers. In my country, nearly 4 million patients with various skin defects need treatment every year, and the treatment cost is as high as tens of billions. Skin trauma is divided into two types: one is minor trauma, which refers to the loss of the epidermis of the skin, such as skin abrasions and first-degree burns, etc.; the other is deep trauma, which damages the epidermis and dermis of the skin, and even damages The intact structure of the skin, such as III degree trauma. Damage to the epidermis can be repaired through the continuous proliferation and differentiation of keratinocytes in the epider...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/44A61L15/42
Inventor 赵剑豪伍煜政许国光容建华
Owner JINAN UNIVERSITY
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