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Two-photon fluorescent probe, fluorescence-labeled kit and detection method used for biopsy of intracellular carbohydrate chain antigen

A sugar chain antigen biopsy and two-photon fluorescence technology, which is applied in the fields of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials, to achieve high clarity and resolution, improve sensitivity and stability, and overcome poor sensitivity and stability Effect

Active Publication Date: 2016-01-13
ZUNYI NORMAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has not been any literature report on the application of two-photon fluorescence to the biopsy of sugar chain antigens of tumors or cancer markers

Method used

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  • Two-photon fluorescent probe, fluorescence-labeled kit and detection method used for biopsy of intracellular carbohydrate chain antigen
  • Two-photon fluorescent probe, fluorescence-labeled kit and detection method used for biopsy of intracellular carbohydrate chain antigen
  • Two-photon fluorescent probe, fluorescence-labeled kit and detection method used for biopsy of intracellular carbohydrate chain antigen

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Embodiment

[0036] The present invention operates according to the following examples:

[0037] The reagents used are: Ab19-9 and Ab125, Shanghai Jianglai Biotechnology Co., Ltd.; PBS: 1.35mol / LNaCl, 47mmol / LKCl, 100mmol / LNa2HPO4, 20mmol / LNaH2PO4, pH7.4, Shanghai Bogu Biotechnology Co., Ltd.; TritonX-100: polyethylene glycol octylphenyl ether, CP, Guangzhou Yinghong Chemical Co., Ltd.; EGTA: ethylene glycol-bis(2-aminoethyl ether) tetraacetic acid, Nanjing Ampere Chemical Technology Co., Ltd.; PIPES: N ,N'-bis(2-ethanesulfonic acid)piperazine, Nanjing Ampere Chemical Technology Co., Ltd.; HEPES: N-2-hydroxyethylpiperazine-N'-2-ethyl phosphoric acid, Nanjing Ampere Chemical Technology Co., Ltd.;

[0038] In addition, other conventional medicines and reagents used in the test are general domestic analytical or chemical pure reagents.

[0039] First, according to the content disclosed in the reference [MoralesAR, YanezCO, Schafer-HalesKJ, MarcusAI, BelfieldKD.Biomoleculelabelingandimagingwi...

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Abstract

The invention relates to the technical field of biopsy of intracellular carbohydrate chain antigen, specifically to a two-photon fluorescent probe, a fluorescence-labeled kit and a detection method used for biopsy of intracellular carbohydrate chain antigen. According to the invention, a two-photon fluorescence labeled antibody LP-IgG and a nitrocellulose-membrane-coated solid-phase antibody Ab are used; during detection, the solid-phase antibody specifically captures to-be-detected antigen through antigen reaction, so an immune complex Ab-Ag is formed; and the two-photon fluorescence labeled antibody LP-IgG is added and fully reacts with the immune complex (LP-IgG.Ab.Ag), then non-specific substances are washed and removed, and results can be detected and determined. The detection method provided by the invention overcomes the disadvantages of poor sensitivity and stability and complicated operation steps in a traditional detection method for carbohydrate antigen; meanwhile, detection samples are no longer limited to blood and peripheral blood, and any sample with carbohydrate chain antigen can be detected by using the kit prepared from the two-photon fluorescence-labeled probe LP.

Description

technical field [0001] The invention relates to the technical field of intracellular sugar chain antigen biopsy, in particular to a two-photon fluorescent probe, fluorescent label kit and detection method for intracellular sugar chain antigen biopsy. Background technique [0002] Carbohydrate antigen (Carbohydrateantigen19-9, CA19-9) is a tumor marker related to pancreatic cancer, gallbladder cancer, colon cancer and gastric cancer, and its monoclonal antibody is 1116NS19-9, referred to as Ab19-9; Carbohydrateantigen125 (CA125) is a tumor marker related to ovarian cancer, fallopian tube adenocarcinoma, endometrial cancer and cervical cancer. Its monoclonal antibody is OC125, Ab125 for short. The determination of CA19-9 is widely used in the clinical monitoring and diagnosis of colorectal cancer, gastric cancer, pancreatic cancer, hepatocellular carcinoma, lung cancer and breast cancer; while the determination of CA125 is widely used in ovarian cancer, fallopian tube adenocar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64G01N33/58
Inventor 黄池宝曾伯平刘其斌张道海
Owner ZUNYI NORMAL COLLEGE
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