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Novel method for determination of escherichia coli residual in chlorine-containing disinfection waste water

A technology for Escherichia coli and chlorine disinfection, which is applied in the field of determination of Escherichia coli residues in chlorine-containing disinfection wastewater, and can solve the problems of low pollution and low cost.

Inactive Publication Date: 2016-01-20
谱尼测试科技(天津)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of Escherichia coli residue determination in chlorine-containing disinfection wastewater, to detect the residue of Escherichia coli in disinfection wastewater, and to avoid biological pollution of environmental water quality by Escherichia coli. The method has low cost, little pollution and high accuracy , which can meet the testing requirements

Method used

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  • Novel method for determination of escherichia coli residual in chlorine-containing disinfection waste water
  • Novel method for determination of escherichia coli residual in chlorine-containing disinfection waste water
  • Novel method for determination of escherichia coli residual in chlorine-containing disinfection waste water

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Embodiment 1

[0015] Analysis steps: Take 300mL of nutrient broth in sterile tanks, prepare 20 tanks, sterilize at 121°C for 15 minutes, add a loop (inoculation loop) of Escherichia coli liquid to each sterile tank, and incubate at 36°C for 24 hours , number each aseptic tank, add 10% sodium hypochlorite solution respectively, as shown in the table below:

[0016]

[0017] Put all the aseptic tanks for 2 hours (disinfectant action time) and then aseptically take 2mL to measure the value of free residual chlorine (GB / T5750.11-2006), and aseptically take 10mL in 10mL double lactose peptone Among them, 5 copies were inoculated in parallel, cultured at 36°C for 24 hours, and the acid and gas production were observed. Those without acid and gas production continued to culture for 24 hours, and a ring of acid and trachea production tubes was picked and drawn on the eosin-methylene blue agar plate, 36 Incubate at ℃ for 24 hours, observe whether there are typical colonies, pick a single typical ...

Embodiment 2

[0022] Analysis steps: Aseptically take 2 mL of domestic sewage sterilized with chlorine and measure the value of free residual chlorine to be 164 mg / L. To determine the presence of Escherichia coli residues, add 1 mL of 0.11 g / L sodium thiosulfate solution to 1 L of water sample, take 10 mL in double lactose peptone, inoculate 5 copies in parallel at the same time, and produce acid and gas after incubation at 36 °C for 24 hours, Pick a ring and streak it on the eosin methylene blue plate, culture it at 36°C for 24 hours, observe the colonies with black center and metallic luster, pick 5 single colonies and inoculate them in IMViC (used to determine the biochemical characteristics of Escherichia coli 4 (a) for biochemical identification and Gram staining. After 24 hours of incubation at 36°C, indigo matrix was positive, methyl red test was positive, acetylmethylmethanol test was negative, citrate test was negative, and Gram staining was negative without bacillus. . In line wi...

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Abstract

The invention discloses a novel method for determination of escherichia coli residual in chlorine-containing disinfection waste water. The novel method comprises 1, determining the amount of free chlorine residual in water, 2, preliminarily determining if the residual escherichia coli exist according to the free chlorine residual value, 3, adding a certain amount of sodium hyposulfite with a concentration of 0.11g / L into the water sample which may contain escherichia coli so that the free chlorine residues are reduced by sodium hyposulfite, and 4, carrying out bacterium propagation, separation and biochemical identification on the water sample and giving a report for showing escherichia coli existence according to the identification result according with escherichia coli characteristics. The novel method has the advantages of simple processes, operation easiness, small pollution, high accuracy and small interference.

Description

【Technical field】 [0001] The invention relates to the technical field of water quality biological detection, in particular to a new method for determining the residual Escherichia coli in chlorine-containing disinfection wastewater. 【Background technique】 [0002] Escherichia coli (dachangganjun) (Escherichiacoli) bacterial phylum. The cells are rod-shaped, about 1 micron in diameter, about 2 microns long, blunt at both ends, with flagella all over the body, which can move. Gram stain negative, no spores formed. The colonies are round, white or yellow-white, smooth and shiny, low or slightly raised, with neat edges. One generation can be reproduced by culturing for 20 minutes under optimal conditions. Escherichia coli is a ubiquitous bacterium in the intestinal tract of humans and warm-blooded animals, and is the main species in feces. Generally living in the human large intestine does not cause disease, and may play a role in the synthesis of vitamin K in the intestine....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
Inventor 宋薇徐丽清
Owner 谱尼测试科技(天津)有限公司
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