Method for improving activity of 2-keto-L-gulonic acid fermentation strains

A technology of fermenting bacteria and gulong acid, which is applied in the field of microbial fermentation, to achieve the effects of enhancing the vitality of bacteria, facilitating production and reproduction, and less damage to nutrients

Inactive Publication Date: 2016-01-27
DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant literature report on enhancing the activity of 2-keto-L-gulonic acid fermentation strains through nitrogen and carbon separation

Method used

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Embodiment Construction

[0015] The present invention will be further described below in conjunction with specific embodiments. The present invention will be further described below in conjunction with specific embodiments.

[0016] 1. Seed pot medium formula: 1.7% sorbose, 0.5% corn steep liquor, 0.3% yeast extract, 0.2% urea, 0.02% magnesium sulfate, 0.1% potassium dihydrogen phosphate, 0.5% calcium carbonate, PH6.7-7.2. 1. Seed pot medium formula: 1.7% sorbose, 0.5% corn steep liquor, 0.3% yeast extract, 0.2% urea, 0.02% magnesium sulfate, 0.1% potassium dihydrogen phosphate, 0.5% calcium carbonate, PH6.7-7.2.

[0017] 2. Seed tank culture conditions: culture temperature 28-30 ℃, control dissolved oxygen 30-50% during the culture process, inoculum volume 10%. 2. Seed tank culture conditions: culture temperature 28-30 ℃, control dissolved oxygen 30-50% during the culture process, inoculum volume 10%.

[0018] 3. Disinfection method of seed tank culture medium: L-sorbose, the main carbon source of ...

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Abstract

The invention discloses a method for improving the activity of 2-keto-L-gulonic acid fermentation strains, and belongs to the technical field of microbial fermentation. The method includes separately disinfecting nitrogen sources and carbon sources in culture media in 2-keto-L-gulonic acid seed tanks; cooling the nitrogen sources and the carbon sources until the temperatures of the nitrogen sources and the carbon sources range from 28 DEG C to 30 DEG C and mixing the nitrogen sources and the carbon sources with one another. The method has the advantages that harmful substances can be reduced by the aid of a process for separately disinfecting nitrogen and carbon, accordingly, nutritious components of the culture media are rarely destructed, the method is favorable for production and reproduction of the production strains, the activity of the strains can be improved, and the 2-keto-L-gulonic acid fermentation periods can be shortened.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and more specifically relates to a method for enhancing the activity of 2-keto-L-gulonic acid fermentation strains. The invention belongs to the technical field of microbial fermentation, and more specifically relates to a method for enhancing the activity of 2-keto-L-gulonic acid fermentation strains. Background technique [0002] Vitamin C (VC) is a water-soluble vitamin, also known as L-ascorbic acid, which can participate in various hydroxylation reactions in the body. Vitamin C (VC) is a water-soluble vitamin, also known as L-ascorbic acid, which can participate in various hydroxylation reactions reaction [0003] It is an important class of redox compounds in cell metabolism and plays an essential physiological role in organisms. Vitamin C is used to prevent scurvy, adjuvant treatment of various acute and chronic infectious diseases and purpura, etc. Vitamin C is also wid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/60
Inventor 陈红胡少斌常芳
Owner DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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