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Two diluent indicators in ELISA detection reagent kit

A technology of indicator and diluent, applied in the field of analysis and detection, can solve the problems of difficult material coloring and so on

Inactive Publication Date: 2016-01-27
赖平安
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the selected indicator must be bright in color and good in water solubility, and it is not easy to color the operator's skin and the contacted materials, and it is not easy to adhere to the inner wall of the enzyme label reaction well and the test table; The detection result of the box has no effect on the judgment

Method used

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  • Two diluent indicators in ELISA detection reagent kit
  • Two diluent indicators in ELISA detection reagent kit

Examples

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example 1

[0009] Example 1 Verification results of foreign bovine tuberculosis gamma interferon ELISA detection kit

[0010] 1. Experimental method and steps

[0011] (1) Preparation of indicators: Brilliant blue and basic blue 26 were respectively prepared into 0.5 mg / ml aqueous solutions.

[0012] (2) Preparation of diluent: add 120ul of basic blue 26 aqueous solution (0.5mg / ml) to the plasma diluent in the kit per 6ml; add 120ul per 6ml of the antibody-enzyme complex diluent in the kit Brilliant blue aqueous solution (0.5mg / ml);

[0013] (3) Dilute the sample and the antibody-enzyme complex with two newly prepared diluents, and simultaneously test 22 samples with the diluent corresponding to the original kit, measure the OD value of the sample, and judge the result according to the kit standard.

[0014] 2. Experimental results

[0015] The ELISA test results of the two dilutions of the newly configured bright blue and basic blue 26 are basically the same as the original ELISA tes...

example 2

[0018] Example 2 Validation results of domestic swine fever antibody ELISA detection kit

[0019] 1. Experimental method and steps

[0020] (1) Preparation of indicators: Brilliant blue and basic blue 26 were respectively prepared into 0.5 mg / ml aqueous solutions.

[0021] (2) Preparation of diluent: add 120ul basic blue 26 aqueous solution (0.5mg / ml) to the serum sample diluent in the kit for every 6ml; add the antibody-enzyme complex diluent in the kit to every 6ml 120ul brilliant blue aqueous solution (0.5mg / ml);

[0022] (3) Dilute the sample and the antibody-enzyme complex with two newly prepared diluents, and simultaneously test 32 samples with the diluent corresponding to the original kit, measure the OD value of the sample, and judge the result according to the kit standard.

[0023] 2. Experimental results

[0024] The ELISA test results of the two dilutions of the newly configured bright blue and basic blue 26 are basically the same as the original ELISA test resu...

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Abstract

The invention relates to two diluent indicators in an ELISA detection reagent kit and belongs to the technical field of analysis and detection. When the enzyme-linked immunosorbent assay (ELISA) detection kit is researched, dye indicators without interference effect to the test result is added in relation to the diluents used in every steps of the experiment, the operation error is avoided, and the reliability of the final detection result is ensured. The concrete steps are as follows: (1) dyes of different colors and properties are selected and then dissolved in a solution to prepare the concentrate; (2) each of the indicator content in the diluents is determined by optimized experiments; (3) experiments of ELISA reagent kits of domestic or foreign countries prove that the indicators in the experiments have no influence on the test results; the diluent indicators of the invention solves the problems that the ELISA reagent kit of domestic products in the prior art has diluents without color display difference, and the resulting sampling skip or repeated sampling during the ELISA operation are solved, thereby avoiding the error during test process.

Description

technical field [0001] The invention relates to the invention of an ELISA diluent indicator, belonging to the technical field of analysis and detection. Background technique [0002] ELISA detection technology is widely used in the detection of human, animal and plant diseases and multiple target components in the body; pesticide and veterinary drug residue screening in food safety monitoring and other fields. Because of its simple operation, low cost and small amount of equipment required, high sensitivity and strong specificity, it can be widely used. [0003] In the field of ELISA detection technology, there are mainly two kinds of diluents used in the sandwich method, indirect method or competition method. Different indicators are added to the 2 different diluents in the kit imported from abroad to prevent operators from mixing them. In addition, after the components used in each reaction step are diluted, they are added to the wells of the microtiter plate because of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/536
Inventor 赖平安刘鑫何悦柏亚铎张伟张朝晖汪琳高志强
Owner 赖平安
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