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81results about How to "Not easy to color" patented technology

DNA silver staining method

The invention discloses a DNA silver staining method. The method comprises the following steps: (1) cutting off power supply after finishing electrophoresis, pouring a buffer solution out from an electrophoresis tank, then taking off a gel plate, putting the rubber plate into a porcelain dish filled with distilled water, and rinsing the gel plate by the distilled water for 2 to 3 times; (2) carrying out silver staining: adding a staining agent to carry out silver staining, shaking ceaselessly, staining for 8 to 12 minutes, and then rinsing by the distilled water for 2 to 3 times; (3) carrying out developing: adding a developing agent to carry out color development, shaking ceaselessly, and developing for 8 to 12 min; (4) rinsing by the distilled water for 2 to 3 times; and (5) carrying out gel shooting: spreading gelatin onto a X-ray film viewer to take a photo. The method integrates the fixing step and the staining step, adopts ethanol to fix, and overcomes the defects that the belt color is lighter when acetic acid is used for fixing, and the acetic acid has penetrating odor, causticity and so on in the prior sliver staining method. Additionally, the method does not need use ethanol and/ or acetic acid to stop developing color, omits the color development stopping step, and solves the problems of penetrating odor and causticity brought by the acetic acid and the like.
Owner:HENAN UNIV OF SCI & TECH

Polycarbonate resin and manufacturing method

The present invention provides a novel polycarbonate resin which is excellent in heat resistance and mechanical strength, is not easily colored during polymerization and molding, and is excellent in transparency and color tone. A polycarbonate resin containing a structural unit derived from a dihydroxy compound represented by the following formula (1), the dihydroxy compound represented by the formula (1) having a boric acid content of 100 ppm by weight or less and / or the tertiary amine content is 1000 ppm by weight or less, and further has a terminal phenyl group derived from the carbonic acid diester represented by the following formula (2), and the terminal phenyl group concentration is 30 μeq / g or more. (In formula (1), R 1 , R 2 , R 3 , R 4 Each independently represents a hydrogen atom, an alkyl group having 1 to 10 carbon atoms, an alkoxy group having 1 to 10 carbon atoms, a cycloalkyl group having 3 to 20 carbon atoms, and a cycloalkoxy group having 6 to 20 carbon atoms. , an aryl group having 6 to 10 carbon atoms, an aralkyl group having 7 to 20 carbon atoms, an aryloxy group having 6 to 10 carbon atoms, an aralkoxy group having 7 to 20 carbon atoms, or a halogen atom. The cyclobutane ring represents any of a mixture of cis and trans isomers, a single cis isomer, and a single trans isomer) (in formula (2), R 5 , R 6 each independently a substituted or unsubstituted aromatic group)
Owner:TEIJIN LTD
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