The invention provides a production method of pure hemp mercerized yarn. By the production method, the mercerized pure hemp yarn has few broken ends, excellent brute force and brightness, the deep dyeing property, level-dyeing property and penetrating property are greatly improved, the handle and smoothness are improved, the difficulty in later processes of dyeing, weaving and after finishing is reduced, and the application performance of the pure hemp yarn is integrally improved. The production method comprises the processes of singeing, reeling, mercerizing, dyeing, squeezing water, drying and rewinding, and is characterized in that the mercerizing process is as follows: yarn is put into a yarn mercerizing machine, wherein the alkalinity of treating fluid is 28-30 baume degrees, the temperature of the treating fluid is 18-22 DEG C, the applied tensile force stretching is 1-3%, the alkali dipping time is 1-3 minutes, the alkali squeezing time is 30 seconds-1 minute, time for washing with hot water is 30-60 seconds at the water temperature of 60-70 DEG C, time for washing with cold water is 30-45 seconds, and the treating time is 5 minutes-5 minutes and 50 seconds.