Cell scratch testing device

Abstract

The invention discloses a cell scratch testing device. The cell scratch testing device comprises a one-dimensional automatic translation stage and a scratching device. A cell culture dish is fixed on the one-dimensional automatic translation stage, and the one-dimensional automatic translation stage comprises a stepping motor which is used for driving the cell culture dish to slide horizontally and controlling movement direction, displacement and speed of the cell culture dish. The scratching device comprises a scratching probe fixture, a scratching probe and a loading device, wherein the scratching probe and the loading device are fixed through the scratching probe fixture, the loading device is used for loading perpendicular acting force on the scratching probe, and the scratching probe fixture enables the scratching probe to be loaded along the perpendicular direction of the bottom face of the cell culture dish. The cell scratch testing device has the advantages that pulse signals generated by a single chip microcomputer control the stepping motor to rotate according to setting directions and angles, so that the cell culture dish on the stage is driven to move precisely; the scratching probe acts on the cell culture dish perpendicularly after being fixed by the fixture; the loading system precisely controls force applied on the scratching probe so as to guarantee cell scratch testing uniformity; uncertainties caused by manual operation are eliminated.

Description

【Technical field】 [0001] The invention relates to the field of cell viability performance testing, in particular to a cell scratch testing device. 【Background technique】 [0002] Cell migration is directional movement through cell body deformation, including a series of material bases such as cytoskeleton, binding protein and intercellular substance. At the same time, the physiological activities of cells, such as cell foraging, wound healing, embryogenesis, immune response, infection, and cancer metastasis, are also closely related to cell migration behavior. [0003] Among them, the cell scratch test is a simple and easy method to detect cell movement. Through the cell scratch test, the migration ability of cells can be detected by simulating cells stimulated by external force or foreign substances. At the same time, it can also be used to evaluate the ability of cells to repair and proliferate after injury under the action of mechanical force. The cell scratch experime...

AI Technical Summary

Problems solved by technology

However, due to the uncertainty of manual operation, this method is likely to cause scratches with irregular shapes and unclear edges.

In addition, due to the individual differences of the force-applying subjects, it is difficult to keep the applied force consistent, which has caused great difficulties in the analysis and statistics of the follow-up experimental results.

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