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A cultivation method for improving root-knot nematode resistance of northern facility tomato

A technology of anti-root-knot nematode and cultivation method, which is applied in the field of improving the cultivation of tomatoes in northern facilities against root-knot nematode, and can solve the problem that the effect of mycorrhizal fungi is not very prominent, mycorrhizal fungi cannot be directly cultured in vitro, and mycorrhizal fungi are independent To improve the anti-infection ability, improve the micro-ecological environment of the rhizosphere, and reduce the damage of pathogenic microorganisms

Inactive Publication Date: 2018-04-20
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since mycorrhizal fungi cannot be directly cultured in vitro, the application of mycorrhizal fungi to tomatoes is severely restricted. At present, inoculation of mycorrhizal fungi and tomato seedlings is the preferred method for tomato seedling cultivation, but the performance of mycorrhizal fungi in the field is not good. Not very prominent, indicating that mycorrhizal fungi have a low independent contribution to the rhizosphere microbial environment and must work together with other microorganisms

Method used

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  • A cultivation method for improving root-knot nematode resistance of northern facility tomato

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Embodiment 1

[0022] 1. Preparation of tomato seedling substrate: Take 3 parts of rice husk and 2 parts of fermented chicken manure according to the volume ratio (preparation by composting fermentation method: in a place with good ventilation and high terrain, pile up chicken manure with litter and use it outside. The mud is closed; the fermentation time is about 15 days in summer and about 50 days in winter), 2 parts of river sand with a particle size of 1 mm and 1 part of perlite with a particle size of 1 mm (rice husk: fermented chicken manure: river sand: perlite = 3:2:2:1), mix evenly to form a mixture, and then sterilize the mixture at 121°C for 50 minutes, take it out and cool it to 40°C to prepare a seedling-raising substrate, and set it aside.

[0023] 2. The preparation method of the solid inoculum of Glomus mosesi: take the river sand passed through a 2mm sieve as the substrate, after high-temperature sterilization (121°C high-pressure steam sterilization for 45 minutes each time)...

Embodiment 2

[0031] 1. Preparation of tomato seedling substrate: Take 2 parts of rice husk, 1 part of fermented chicken manure, 1 part of river sand with a particle size of 1 mm and 1 part of perlite with a particle size of 1 mm according to the volume ratio (rice husk: fermented chicken manure: River sand: perlite=2:1:1:1), mix evenly to form a mixture, then sterilize the mixture at 121°C for 40 minutes, take it out and cool it to 30°C to prepare a seedling-raising substrate, and set aside.

[0032] 2. The preparation method of the solid bacterial agent of Glomus mosesi: take the river sand passed through a 2mm sieve as the substrate, after high-temperature sterilization (121°C high-pressure steam sterilization for 45 minutes each time), place it in a natural state for 14 days, and then pack it in two 1 kg into a pot, inoculated with 100 g of the original species of Glomus moses, mix well, add 20% of the weight of the substrate in tap water, sow the seeds of sorghum and scallions, keep nor...

Embodiment 3

[0038] 1. Preparation of tomato seedling substrate: Take 4 parts of rice husk, 3 parts of fermented chicken manure, 1 part of river sand with a particle size of 1 mm and 1 part of perlite with a particle size of 1 mm according to the volume ratio (rice husk: fermented chicken manure: River sand: perlite=4:3:1:1), mix well to form a mixture, then sterilize the mixture at 121°C for 40 minutes, take it out and cool it to 30°C to prepare a seedling-raising substrate, and set aside.

[0039] 2. The preparation method of Glomus moses solid inoculum: take the river sand passing through a 2mm sieve as the substrate, after high-temperature sterilization (121°C high-pressure steam sterilization for 45min each time), place it in a natural state for 10 days, and then take two 1 kg into a pot, inoculate 150g of Glomus moses original species, mix well, add tap water with 18% of substrate weight, sow sorghum seeds, keep normal growth, and get the host infected by Glomus moses after six months...

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Abstract

The invention discloses a cultivation method for improving northern protected tomato resistibility to root-knot nematode, the method comprising: A. Preparation of seedling substrate: the seedling substrate comprises, by volume, 2-4 parts of rice husk, 1-3 parts of fermented chicken manure, 1-2 parts of river sand, and 1 part of perlite. The seedling substrate is prepared by well mixing and disinfecting those raw materials. B. Mycorrhizal fungi is inoculated on the seedling substrate to form tomato mycorrhizal inoculant, then, tomato seeds are sowed and cultured on the tomato mycorrhizal inoculant, and tomato seedlings are sprouted. C. Before tomato seedling transplanting, phosphorus solubilizing bacteria is inoculated at root sites of tomato seedlings; tomato mycorrhizal inoculant is transplanted together with the tomato seedlings when transplanting. Soil is covered. Cultivation management is performed to tomato seedlings. The cultivation method has advantages of simple technology and easy operation so that industrialization production of tomato seedlings is facilitated.

Description

Technical field: [0001] The invention belongs to the field of agricultural production and microbe application, and in particular relates to a cultivation method for improving the root-knot nematode resistance of northern facility tomatoes. Background technique: [0002] Tomato is one of the main economic crops in our country and it is the guarantee for farmers to increase their income. The higher the intensification degree of facility vegetable tomato, the more serious the obstacle of continuous cropping in tomato rhizosphere soil, especially the flood of root-knot nematode. At present, soil-borne diseases in tomato planting are serious in our country. Continuous cropping causes the soil quality of tomato planting to decline, and the yield of root-knot nematode is reduced by 30% every year, accounting for more than 40% of the tomato planting area. Restricting tomato intensive production. In areas with a relatively high degree of intensive planting of protected vegetables i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G18/00
CPCA01G18/10A01G24/00
Inventor 丁效东张士荣李俊良
Owner QINGDAO AGRI UNIV
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