Fluorescence system with fluorescence intensity insensitive to temperature
A fluorescent intensity and sensitive technology, applied in the field of analytical chemistry, can solve problems such as complex effects and no fluorescence
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Embodiment 1
[0049] 1. Synthesis of intermediate 4-dimethylamino-2-hydroxybenzaldehyde (fluorescent molecule 3)
[0050]
[0051] 30mmol of POCl at 0°C 3 It was slowly added dropwise into anhydrous DMF, and after stirring for 30 minutes, 20 mmol of 3-dimethylaminophenol previously dissolved in anhydrous DMF was slowly added dropwise into the solution, and the temperature was raised to 80°C. After reacting overnight, the reaction solution was poured into ice water, adjusted to neutrality with sodium hydroxide solution, and filtered to obtain a solid. The solid was dried in a vacuum oven for 6 hours, and the yellow solid 4-(dimethylamino)-2-hydroxybenzaldehyde was separated by column chromatography. The separation condition of the column chromatography was petroleum ether:ethyl acetate=3:1 .
[0052] The proton data of this intermediate are as follows: 1 HNMR (400MHz, CDCl 3 )δ11.59(s,1H),9.52(s,1H),7.28(d,J=8.9Hz,1H),6.28(dd,J=8.8Hz,2.4Hz,1H),6.07(s,1H) ,3.06(s,6H).
[0053] 2. Syn...
Embodiment 2
[0066] Normalized UV-vis absorption of fluorescent molecule 1 at different concentrations in chloroform solution:
[0067] Weigh 1.086 mg of 7-dimethylamino-3-formyl coumarin (fluorescent molecule 1), dissolve it in 5 ml of dichloromethane, make a mother solution with a concentration of c=1 mmol / L, and take four 5 ml centrifuge tubes , add 8 μL, 16 μL, 32 μL, and 80 μL of the mother solution into the centrifuge tubes, and after the dichloromethane in the tubes evaporates to dryness, add 4 ml of chloroform to the four centrifuge tubes to prepare the concentrations of 2 μM, 4 μM, 8 μM, and 20 μM. The solution to be tested was mixed uniformly and its UV absorption spectrum was measured.
[0068] The ultraviolet measuring instrument used is Cary60UV-Vis ultraviolet spectrophotometer. From Figure 6 After normalization, it can be seen that the overlap of ultraviolet absorption spectra of different concentrations is very good, and it can be seen that the aggregation of molecules i...
Embodiment 3
[0070] Two-dimensional absorption-emission spectrum of fluorescent molecule 1 in chloroform solution:
[0071] Weigh 1.086 mg of 7-dimethylamino-3-formyl coumarin (fluorescent molecule 1), dissolve it in 5 ml of dichloromethane, make a mother solution with a concentration of c=1 mmol / L, and add it to a 5 ml centrifuge tube 8 μL of mother liquor, after the dichloromethane in the tube evaporates to dryness, add 4ml of chloroform to the centrifuge tube to make a test solution with a concentration of 2 μM, mix well and measure its two-dimensional absorption-emission spectrum. The fluorescence measuring instrument used is the CaryEclipse Fluorescence Spectrophotometer fluorescence spectrophotometer. From Figure 7 The two-dimensional absorption-emission spectrum in chloroform solution shows that only one main peak can be seen, thus indicating that there is only one species in the solution.
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