A plum halogen essence functional food rich in phenolic substances and organic acids and its preparation method
A technology for phenolic substances and functional food, which is applied in the field of umeboshi essence functional food and its preparation, can solve the problems of high energy consumption and low content of biologically active substances, and achieves high added value, low cost and improved utilization rate. Effect
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Embodiment 1
[0034] Such as figure 1 As shown in, the preparation method of the plum halogen essence functional food rich in phenolic substances and organic acids provided by the present embodiment comprises the following steps:
[0035] (1) Take 100L plum brine, filter it with a 100-mesh nylon filter cloth, and centrifuge at 10,000 rpm to obtain clarified plum brine (wherein the filtrate is diluted and discharged or sodium chloride is recovered); The treatment method is provided by the resin production company, mainly X-5 macroporous adsorption resin soaked in high-concentration ethanol, rinsed with sodium hydroxide solution and dilute hydrochloric acid, etc., collect the transudate 1 for later use, and detect the phenolic substances in the transudate 1 , using the Folin-phenol method to detect phenolic substances in the transudate 1, and stop adding plum brine when a positive reaction occurs;
[0036] (2) Rinse the resin column with pure water until the washing liquid is clarified, elut...
Embodiment 2
[0044] Such as figure 1 As shown in, the preparation method of the plum halogen essence functional food rich in phenolic substances and organic acids provided by the present embodiment comprises the following steps:
[0045] (1) Take 100 L of plum brine, filter it with 300 mesh nylon filter cloth, and centrifuge at 8000 rpm to obtain clarified plum brine; pump the clarified plum brine into the pretreated AB-8 macroporous adsorption resin, collect the leakage liquid 1 for subsequent use, And detect the phenolic substances in the transudate 1, and stop adding plum brine when a positive reaction is shown;
[0046] (2) Rinse the resin column with pure water until the washing liquid is clarified, elute with 50% (volume percentage) ethanol aqueous solution containing 0.1% hydrochloric acid (volume percentage), collect the eluate, concentrate and reclaim ethanol, and obtain rich For the extract containing phenolic substances, the total phenol content of the obtained phenolic substan...
Embodiment 3
[0052] Such as figure 1 As shown in, the preparation method of the plum halogen essence functional food rich in phenolic substances and organic acids provided by the present embodiment comprises the following steps:
[0053] (1) Take 100 L of plum brine, filter it with 80 mesh nylon filter cloth, and centrifuge at 6000 rpm to obtain clarified plum brine; pump the clarified plum brine into the pretreated X-5 macroporous adsorption resin, collect the transudate 1 for later use, And detect the phenolic substances in the transudate 1, and stop adding plum brine when a positive reaction is shown;
[0054] (2) Rinse the resin column with pure water until the washing liquid is clear, elute with 30% (volume percentage) methanol aqueous solution containing 0.12% hydrochloric acid (volume percentage), collect the eluate, concentrate and recycle methanol, and obtain rich For the extract containing phenolic substances, the total phenol content of the obtained phenolic substance-rich extr...
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