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Magneto-optical double-mode imaging nanoprobe and application thereof

A magneto-optical dual-mode, nano-probe technology, applied in the field of biomedicine, can solve the problem of not being able to provide information on the structure and function of biological tissues, achieve good blood circulation time in the body, good clinical transformation prospects, and easy surface functional modification Effect

Active Publication Date: 2016-02-17
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, these imaging methods can only provide preoperative tumor detection, but cannot provide real-time biological tissue structure and function information during the operation. The success of the operation depends largely on the subjective judgment of the surgeon, so there is a relatively large risk

Method used

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  • Magneto-optical double-mode imaging nanoprobe and application thereof
  • Magneto-optical double-mode imaging nanoprobe and application thereof
  • Magneto-optical double-mode imaging nanoprobe and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] NIR Ag 2 S quantum dots: The surface ligand is lipoic acid, with an average of about 5.4nm. The preparation method is as follows: 0.1 mmol of silver diethyldithiocarbamate and 10 g of dodecanethiol are mixed and placed in a flask, and placed in a flask under N 2 The temperature was raised to 200°C in the atmosphere and kept for 1 hour. Finally, after the solution was naturally cooled to room temperature, 50 mL of absolute ethanol was added, centrifuged, washed, dispersed in cyclohexane, and then an equal volume of absolute ethanol was added to the solution in an ultrasonic cleaner. Sonicate for 4h, then centrifuge, and wash with deionized water to obtain water-soluble Ag with a particle size of about 5nm 2 S quantum dots.

[0032] Preparation of a magneto-optical dual-mode nanoprobe: a near-infrared Ag 2 S quantum dots, Gd-DOTA, are assembled by amino-functionalized PEG molecules. Among them, Gd-DOTA was purchased from Sigma, and amino-functionalized modified PEG wa...

Embodiment 2

[0044] NIR Ag 2 Se quantum dots: the surface ligand is C18-PMH-PEG, with an average size of 3.5nm. The preparation method is as follows: add 0.04mmol Ag-Oleylamine, 7mL toluene, 3mL dodecanethiol, and 7mL 0.01mmol / L NaHSe solution into a 50mL reactor and stir for 5min, then place it at 180°C for 1h. Then use 60mL absolute ethanol, centrifuge, wash, disperse in chloroform, then add 50mgDSPE-PEG-NH 2 (purchased from Nanocs) and stirred for 12h, finally washed by centrifugation and dispersed in water.

[0045] Fabrication of magneto-optical dual-mode nanoprobes: from near-infrared Ag 2 Se quantum dots and Gd-DTAP were assembled by amino-functionalized PEG molecules. Among them, Gd-DTPA was purchased from Sigma, and amino-functionalized modified PEG was purchased from Sunbio.

[0046] Its preparation method is as follows.

[0047] (1) Chemically cross-linked Gd chelate molecules and modified PEG molecules: 50mgGd-DTAP, 100mgDSPE-PEG-NH 2 , add 10 mL containing 75 mM EDC (1-e...

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Abstract

The invention discloses a magneto-optical double-mode imaging nanoprobe and application thereof. The nanoprobe comprises a kernel, a magnetic material and synthetic polymers or biomacromolecules, wherein the kernel is formed by quanta; and the synthetic polymers or biomacromolecules are connected between the kernel and the magnetic material, are connected with the magnetic material through chemical crosslinking reaction, and are connected with the surface of the kernel through a chemical crosslinking process, a hydrophobic force or a Van der Waals' force. Through the magneto-optical difunctional probe, tumor in-situ, real-time and visual diagnosis and fluorescent guided excision can be realized.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a nanoprobe with magneto-optical dual-mode imaging function and its application. Background technique [0002] Cancer is one of the major diseases that threaten human life and health. Due to the limitation of radiation dose and multi-drug resistance of tumors, it is difficult to completely eliminate tumor cells in the body through radiotherapy and chemotherapy. Surgery remains the treatment of choice for the vast majority of solid tumors. Nevertheless, a large number of clinicopathological results show that the occurrence of early tumors is relatively hidden and no obvious lesion area can be found, and the tumors show aggressive and infiltrating growth, resulting in blurred borders with normal tissues, and the tumors are often accompanied by important blood vessels and nerves. etc., thereby bringing great difficulties to the safe and complete excision of the lesion tissue in the oper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/12A61K49/14
Inventor 王强斌李春炎张叶俊董博华
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI