Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Selenium-rich candida glabrata strain FXY-4 as well as cultivation method and application of selenium-rich candida glabrata strain FXY-4 serving as additive for fish feed

A technology of Candida glabrata and FXY-4, which is applied in the field of microorganisms, achieves good application prospects, saves breeding costs and improves economic benefits.

Active Publication Date: 2016-02-17
广州邹鲁农业有限公司
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of high-yield selenium-enriched Candida glabrata in aquaculture feed has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Selenium-rich candida glabrata strain FXY-4 as well as cultivation method and application of selenium-rich candida glabrata strain FXY-4 serving as additive for fish feed
  • Selenium-rich candida glabrata strain FXY-4 as well as cultivation method and application of selenium-rich candida glabrata strain FXY-4 serving as additive for fish feed
  • Selenium-rich candida glabrata strain FXY-4 as well as cultivation method and application of selenium-rich candida glabrata strain FXY-4 serving as additive for fish feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Candida glabrata FXY-4, which can be obtained as follows:

[0021] Isolation of the original strain:

[0022] Dilute the pickle juice sample and spread it on the plate of YEPD medium, cultivate it at 28°C, observe the growth state of the colony at regular intervals, and pick a single colony and transfer it to the YEPD slant for storage. After preliminary screening, the following yeasts are obtained: Strains: FXY-1, FXY-3, FXY-4, FXY-5, FXY-7, FXY-10, FXY-11, FXY-19, FXY-21, FXY-23, FXY-24, FXY-25, FXY-38, FXY-42, FXY-43, FXY-44, FXY-46, FXY-47, FXY-49, FXY-50, FXY-53, FXY-55, FXY-56.

[0023] containing Na 2 SeO 3 (final concentration is 0.5g / L) in the YEPD substratum that carries out the screening of selenium-enriched yeast, then the yeast that preliminary screening obtains is cultivated with YEPD substratum, and every strain bacterium is divided into blank group and experimental group and cultivates, Add Na to the experimental group 2 SeO 3 (final concentration ...

Embodiment 2

[0025] Yeast strain FXY-4 (deposit number: CCTCCNO: M2015664) 18SITSrRNA gene sequence analysis:

[0026] (1) Extraction of chromosomal DNA (small amount):

[0027] 1. Take 1.5mL bacterial liquid in a 1.5mL Eppendorf tube and centrifuge at 12000r / min for 5min;

[0028] 2. Discard the supernatant, resuspend the pellet in 900 μL phosphate buffered solution (PBS), and centrifuge at 12000 r / min at 4 °C for 5 min;

[0029] 3. Discard the supernatant, add 300TE and 200μL 10mg / mL lysozyme, blow and aspirate to mix, incubate at 37°C for 1 hour, and mix by inverting every 15 minutes;

[0030] 4. Add 600TENS lysate (200mmol / LNaCl, 100mmol / LTril-HClpH8.0, 2.0%SDS, 50mmol / LEDTA, 0.5%TritonX-100) and 20mg / mL proteinase K10μL to the pellet, mix upside down, and incubate at 55°C 1h, invert and mix once every 15min;

[0031] 5. Centrifuge at 12000r / min at 5.4°C for 5min, and take the supernatant;

[0032]6. Add an equal volume of P:C:I (25:24:1) to the supernatant, mix thoroughly, and cen...

Embodiment 3

[0060] Yeast FXY4 (Deposit number: CCTCCNO: M2015664), BioMérieux VITEK2 automatic identification analysis

[0061] Pick the bacterial lawn of the strain to be tested and inoculate it on the YEPD plate, cultivate it to the logarithmic growth phase at 30°C, dip the Vitek liquid in the kit with a sterilized cotton swab, scrape off the bacterial lawn on the plate, and then dissolve it in 1.8ml of Vitek liquid. Shake and mix well; use a turbidimeter to adjust the turbidity to the specified range (2MacF); then use a pipette gun to add the bacterial suspension to the Vitek identification plate, and the bacterial solution should fill the identification well; then incubate at 30°C for 24 to 48 hours Take out the identification plate, and use the BioMérieux VITEK2 automatic identification analyzer to read and analyze the data of the identification plate. The results are shown in Table 3. The physiological and biochemical results are the same as the typical physiological and biochemical ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a selenium-rich candida glabrata strain FXY-4 as well as a cultivation method and an application of selenium-rich candida glabrata strain FXY-4 serving as an additive for fish feed and belongs to the technical field of microorganisms. A pickle juice sample is diluted, a YEPD (yeast extract peptone dextrose) culture medium is coated with the pickle juice sample for cultivation, single colonies are picked out, selenium-rich saccharomycete is screened through the YEPD culture medium containing Na2SeO3 with the final concentration being 0.5g / L, and the selenium-rich candida glabrata strain FXY-4 is obtained with a chemical mutagenesis method and classified and named as candida glabrata FXY-4, has been preserved in CCTCC (China Center For Type Culture Collection) and has the preservation number CCTCC NO:M 2015664. The selenium-rich candida glabrata FXY-4 has remarkable immunity enhancing and growth promoting functions, and an animal experiment proves that the selenium-rich candida glabrata strain FXY-4 has a promoting function on growth and health of aquatic animals and can be used as the additive to be applied to the fish feed.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular, the invention relates to a selenium-enriched Candida glabrata strain FXY-4, a cultivation method thereof and an application as a fish feed additive. Background technique [0002] Selenium (Se) is an essential trace element for animals and plays an important role in maintaining animal health, but excessive selenium can cause toxicity to the body, so research on the optimal amount of selenium in animal feed has been extensively carried out. Nutritional research on selenium in fish feed shows that too high or too low selenium content in feed will affect the health of fish. In the process of intensive aquaculture, some pathogenic bacteria, especially intestinal conditional pathogens, can multiply rapidly and cause corresponding diseases. At present, the main method is to control and reduce stress by adding antibiotics in the feed. Antibiotic supplementation can inhibit the gro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A23K10/18A23K50/80C12R1/72
Inventor 代俊陈雄张毅俞灿王志李欣周梦舟唐景峰朱莹
Owner 广州邹鲁农业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com