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Recombinant porcine pseudorabies virus strain expressing PPV VP2 gene and porcine IL-18 gene

A porcine pseudorabies virus and gene technology, applied in the direction of virus/bacteriophage, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of economic loss and aggravation of harm in the pig industry

Inactive Publication Date: 2016-03-02
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

And it is often mixed with other pathogens such as porcine pseudorabies virus, swine fever virus, porcine reproductive and respiratory syndrome virus, porcine circovirus and other pathogens to aggravate its harm and bring huge economic losses to the pig industry

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  • Recombinant porcine pseudorabies virus strain expressing PPV VP2 gene and porcine IL-18 gene
  • Recombinant porcine pseudorabies virus strain expressing PPV VP2 gene and porcine IL-18 gene
  • Recombinant porcine pseudorabies virus strain expressing PPV VP2 gene and porcine IL-18 gene

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Embodiment Construction

[0049] A recombinant porcine pseudorabies virus strain expressing PPVVP2 gene and porcine IL-18 gene, classified and named: recombinant porcine pseudorabies virus PRV-PPV-IL18PGVP218 expressing PPVVP2 gene and porcine IL-18 gene, Latin name: Herpesviridae. Deposit unit: China Center for Type Culture Collection, deposit unit abbreviation: CCTCC, deposit unit address: Wuhan University, Wuhan, China, deposit date: November 18, 2013, deposit number CCTCC NO: V201347.

[0050] 1. Construction of recombinant PRV transfer plasmid expressing PPV Henan strain VP2 gene and porcine IL-18 gene

[0051] 1 Materials and methods

[0052] 1.1 Materials

[0053] 1.1.1 Plasmids, strains and viruses

[0054] The universal transfer vector pG contains the PRVgG promoter and the inserted hCMV gene, SV40Poly(A) gene and enhanced green fluorescent protein (EGFP) gene, purchased from the Key Laboratory of Animal Food Safety in Henan Province; it contains the recombinant pig IL-18 gene Plasmid pIRES...

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Abstract

The invention discloses a recombinant porcine pseudorabies virus strain expressing a PPV VP2 gene and a porcine IL-18 gene. The recombinant porcine pseudorabies virus (Herpesviridae) strain is numbered as CCTCC NO.V201347. The recombinant porcine pseudorabies virus PGVP218 established by the recombinant porcine pseudorabies virus strain and an IL-18-free recombinant pseudorabies virus rPRV-VP2 established in a laboratory respectively immunize rats, meanwhile a DMEM culture solution is used as a negative control and a PPV HB98 vaccine and a PRV inactivated vaccine are used as a positive control group to immunize the rats, and the immunogenicity of the recombinant virus is studied through PPV specific antibody detection, a PRV antibody neutralization test, peripheral blood T lymphocyte subpopulation determination and a attacking protection test. Results show that the recombinant virus PGVP218 is safe and effective to the rats, can induce the rats to produce specific PPV and PRV antibodies, and the number of CD4+ / CD8+T lymphocyte subpopulations is increased.

Description

technical field [0001] The invention relates to a recombinant porcine pseudorabies virus strain expressing PPVVP2 gene and porcine IL-18 gene. Background technique [0002] Porcine parvovirus (Porcineparvovirus, PPV) is one of the main pathogens that cause reproductive disorders in sows. It can cause early embryonic death, mummification, stillbirth, infertility and delayed estrus. Myocarditis and wasting syndrome. And it is often mixed with other pathogens such as porcine pseudorabies virus, swine fever virus, porcine reproductive and respiratory syndrome virus, porcine circovirus and other pathogens to aggravate its harm and bring huge economic losses to the pig industry. [0003] Porcine pseudorabies virus is also one of the main pathogens causing reproductive disorders in pigs. Its hazards are mainly manifested as sow abortion, stillbirth, mummified fetus and neonatal piglet death and other syndromes. Clinically, stillbirth is the main cause. Pseudorabies virus (PRV) h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12R1/93
Inventor 陈红英杨明凡王林青付朋飞乔涵杨兴武李新生
Owner HENAN AGRICULTURAL UNIVERSITY
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