Molecular identification method of Dian-type I three-line hybrid Japonica rice sterile line

Abstract

The invention discloses a molecular identification method of a Dian-type I three-line hybrid Japonica rice sterile line. Two pairs of primers (csSPF and csSPR1) and (RFSPF2 and RFSPR21) are designed in dependence on sterile gene in mitochondrial genome in cytoplasm of a Dian-type I Japonica rice sterile line and restoring gene sequences in cell nuclei in the Dian-type I Japonica rice sterile line, respectively, PCR (polymerase chain reaction) amplification is carried out using the two pairs of primers, the Dian-type I Japonica rice sterile line can be accurately judged according to amplification results of the two pairs of primers and distinguished from a maintainer line, a restoring line and a hybrid, and efficient, quick and accurate identification of the sterile line is achieved. Compared with the traditional art, the method has unique design, is simple and is high in accuracy, can fully meet current development requirements, enables more accurate molecular positioning for fertile genes and gives promotion to the development of the gene molecular identification field.

Description

technical field [0001] The patent of the invention belongs to the field of breeding in the field of agricultural biotechnology, and in particular relates to a molecular identification method for a Dian-1 type three-line hybrid japonica rice sterile line. technical background [0002] Rice is one of the most important food crops in my country. The breeding of hybrid rice has made great contributions to the solution of food production and food and clothing problems in my country. The basis for the effective use of rice heterosis is the selection and application of male sterile lines. One of them is cytoplasmic male sterility controlled by the interaction of nuclear genes and cytoplasmic genes, and the other is nuclear male sterility controlled by the interaction of genetics and the environment, including light-sensitive and temperature-sensitive male sterility. At present, there are mainly cytoplasmic male sterile lines in japonica rice that utilize nuclear-cytoplasmic gene in...

AI Technical Summary

Problems solved by technology

The selection accuracy rate decreases rapidly with the increase of the recombination rate. In molecular-assisted selection breeding, if the selection accuracy rate is required to reach more than 90%, the recombination rate between the marker and the target gene must be less than 5%. When the recombination rate reaches 10%, , the accuracy of selection has dropped to 80%

[0004] On the other hand, due to the relative lag in the research on sterile genes, as far as the current molecular identification development technology is concerned, there is still a lack of a method and means that can quickly, effectively and accurately detect the sterile line of Dian-1 japonica rice

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