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A kind of quantitative detection method of chloramphenicol cap in water body

A quantitative detection method, the technology of chloramphenicol, applied in the direction of material excitation analysis, Raman scattering, etc., can solve the problems of difficult detection and lack of binding sites, and achieve the effect of simple operation and stable and reliable detection data

Active Publication Date: 2019-01-11
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chloramphenicol is a typical small molecule substance, which lacks multiple binding sites, and it is difficult to detect it using the traditional "sandwich" structure in Raman immunoassay.

Method used

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  • A kind of quantitative detection method of chloramphenicol cap in water body
  • A kind of quantitative detection method of chloramphenicol cap in water body
  • A kind of quantitative detection method of chloramphenicol cap in water body

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Such as figure 1 Shown, the quantitative detection method of chloramphenicol CAP in the water body that the present embodiment provides, comprises the following steps:

[0051] (1) Preparation of noble metal particles

[0052] Under constant stirring, 100 mL of 1 mM chloroauric acid (HAuCl 4 ) solution was heated to boiling, and then 6 mL of 38.8 mM trisodium citrate aqueous solution was added. At this time, the color change of the solution is: light yellow-colorless-black-purple-dark red, wait for the solution to turn dark red and continue to heat and reflux for 15-20min. Finally cooled to normal temperature, the preparation obtains the noble metal (colloidal gold) microparticle solution of 30nm, and its transmission electron microscope picture sees figure 2 .

[0053] Preparation of noble metal solutions for immunospectral labeling

[0054] Take 1 mL of the precious metal solution (concentration is about 1 mmol / L) freshly prepared in step (1), put it into an EP ...

Embodiment 2

[0069] Get the water body (near farm) of the Guangzhou section of the Pearl River, the method of operation is the same as in Example 1, and its Raman spectrogram of survey is as follows Figure 6 Shown (experiment three times), the signal intensity mean value is 2009 (a.u.), substituted into the linear equation in the embodiment 1, obtains the concentration of chloramphenicol in this actual water body to be 2.29ng / mL.

Embodiment 3

[0071] Get mountain spring water (collected from the foot of Huolu Mountain), operation method is the same as embodiment 1, and its Raman spectrogram of measuring is as follows Figure 7 Shown (experiment three times), signal intensity mean value is 309 (a.u.), and this signal intensity value is relatively close to background signal intensity, can think that in this place water source does not contain chloramphenicol.

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Abstract

The invention discloses a quantitative detection method of chloramphenicol (CAP) in water. The method includes the steps that a precious metal particle solution A subjected to immune spectral signature treatment is prepared, then a magnetic particle solution B modified with CAP antibodies is prepared, then a series of CAP sample solutions with a concentration gradient are prepared, the solution A and the series of CAP sample solutions with a concentration gradient are dripped into the solution B, and thus CAP in the sample solutions and CAP-BSA in the solution A are specifically bound to the CAP antibodies in the solution B at the same time; then, magnetic conjugate is separated through a magnetic field, supernate is kept, signals of raman signal tagged molecules on residual precious metal particles in the supernate are collected, a standard curve is drawn according to the corresponding relation between the concentration of CAP in the sample solutions and the intensity of the raman signals, and thus the concentration of CAP in a water sample to be detected is detected. According to the method, conditions are mild, operation is easy and convenient, the detection process is fast, the detection result is accurate, sensitivity is high, and quantitative analysis of trace CAP can be achieved.

Description

technical field [0001] The invention belongs to the technical field of trace antibiotic detection, and in particular relates to a quantitative detection method for chloramphenicol CAP in water bodies, in particular to a Raman spectrum labeling detection technology for quantitatively detecting chloramphenicol CAP in water bodies through magnetic particles to achieve physical separation Methods. Background technique [0002] Chloramphenicol (CAP) is a bacteriostatic broad-spectrum antibiotic, and it is the first broad-spectrum antibiotic produced in large quantities by synthetic methods in the world. It has a marked effect on many different types of microorganisms. However, due to the side effect of fatal aplastic anemia, it has been banned by most countries in the world. However, in recent years, antibiotic contamination incidents have often occurred, so accurate and rapid detection of antibiotics (chloramphenicol) is of great significance. At present, the detection of chl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 胡勇军杨康董宁朱挺锋余萌
Owner SOUTH CHINA NORMAL UNIVERSITY