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Preparation method of chitosan plasma grafted hyaluronic acid hemostasis biomaterial

A technology of hyaluronic acid and hemostatic material, which is applied in pharmaceutical formulations, drug delivery, medical science and other directions, can solve problems such as wound inflammation, and achieve the effect of preventing hemostasis and exothermic heat, no toxic side effects, and good combination effect.

Inactive Publication Date: 2016-03-16
CHANGZHOU SIYU ENVIRONMENTAL PROTECTION MATERIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention: Aiming at the problem that the current hemostatic material will emit a large amount of heat after absorbing the water in the blood during use, resulting in wound inflammation, a heating reaction between chitosan and formic acid is provided. Under the action of epichlorohydrin, activated chitosan is prepared, and then chitosan is hydrolyzed to prepare hyaluronic acid, and chitosan is grafted with hyaluronic acid to prepare biological hemostatic materials through plasma grafting. less prone to inflammation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] First select 10g of chitosan, put it in a 1L flask, then continue to add 200mL of formic acid solution with a mass concentration of 15%, heat the reaction in an oil bath at 85°C for 2h, and place it at 35°C after the reaction is completed Rotary evaporation to 1 / 10 of the original volume prepares the chitosan formic acid concentrate; the chitosan formic acid concentrate prepared above is placed in a 200mL centrifuge tube, and the volume of the chitosan formic acid concentrate Ratio 1:5, absolute ethanol was added to the centrifuge tube, centrifuged at a speed of 4000r / min for 5min, the lower layer was collected and washed twice with absolute ethanol, the precipitated chitosan was collected and vacuum freeze-dried for 6h, Prepare highly water-soluble chitosan; select 5g of highly water-soluble chitosan prepared above, place it in a 1L flask, then continue to add 100mL of distilled water, place it in a 30°C water bath and heat for 25min, after the heating is completed, ...

example 2

[0021] First select 12g of chitosan, put it in a 1L flask, then continue to add 225mL of formic acid solution with a mass concentration of 15%, heat and react in an oil bath at 87°C for 2.5h, after the reaction is completed, place it at 37°C Rotary evaporation down to 1 / 10 of the original volume prepares the chitosan formic acid concentrate; the chitosan formic acid concentrate prepared above is placed in a 200mL centrifuge tube, and is mixed with absolute ethanol and chitosan formic acid concentrate The volume ratio is 1:5, add absolute ethanol to the centrifuge tube, centrifuge at a speed of 4500r / min for 7min, collect the lower precipitate and wash it twice with absolute ethanol, collect the precipitated chitosan and vacuum freeze-dry it for 7h , to prepare highly water-soluble chitosan; select 5.5g of highly water-soluble chitosan prepared above, place it in a 1L flask, then continue to add 102mL of distilled water, place it in a 35°C water bath and heat for 27min, and wa...

example 3

[0024] First select 15g of chitosan, put it in a 1L flask, then continue to add 250mL of formic acid solution with a mass concentration of 15%, heat the reaction in an oil bath at 90°C for 3h, and place it at 40°C after the reaction is completed Rotary evaporation to 1 / 10 of the original volume prepares the chitosan formic acid concentrate; the chitosan formic acid concentrate prepared above is placed in a 200mL centrifuge tube, and the volume of the chitosan formic acid concentrate Ratio 1:5, add absolute ethanol to the centrifuge tube, centrifuge at a speed of 5000r / min for 10min, collect the lower precipitate and wash with absolute ethanol 3 times, collect the precipitated chitosan and vacuum freeze-dry it for 8h, Prepare highly water-soluble chitosan; select 6g of highly water-soluble chitosan prepared above, place it in a 1L flask, then continue to add 105mL of distilled water, place it in a 40°C water bath and heat for 30min, after the heating is completed, Continue to...

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Abstract

The invention relates to a preparation method of a chitosan plasma grafted hyaluronic acid hemostasis biomaterial, and belongs to the technical field of preparation of biomaterials. The problem of wound inflammations caused by release of a large amount of heat after absorption of water in blood by a hemostasis material in the use process is solved. The preparation method of the chitosan plasma grafted hyaluronic acid hemostasis biomaterial comprises the following steps: carrying out a heating reaction on chitosan and formic acid under the action of chloropropylene oxide to prepare activated chitosan, hydrolyzing chitosan to prepare hyaluronic acid, and carrying out plasma grafting to obtain the chitosan plasma grafted hyaluronic acid hemostasis biomaterial. The biomaterial reduces the proportion of wound inflammations caused by fever by 80% or above; and hyaluronic acid is prepared from chitosan, and the combination effect of chitosan and hyaluronic acid is good, so the biomaterial can be completely absorbed by human bodies and has no toxic or side effects.

Description

technical field [0001] The invention relates to a preparation method of a chitosan plasma-grafted hyaluronic acid biological hemostatic material, which belongs to the technical field of biological material preparation. Background technique [0002] In the first aid treatment of sudden accidents, trauma hemostasis in the operation process, especially in the rescue of the wounded in war, it is very important for the patient to effectively stop the bleeding locally and quickly. Rapid hemostasis materials can effectively reduce casualties. Currently reported rapid hemostasis materials mainly include powders (zeolite, collagen powder and potato starch), porous sponges (gelatin sponge, collagen sponge), oxidized regenerated cellulose and fibrin glue, etc., each of which has certain shortcomings. Gelatin and collagen have poor tissue adhesion, and the hemostatic function of both depends on sufficient platelets and coagulation factors, so their applications are limited. [0003] F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00C08B37/08A61L26/00
CPCC08G81/00A61L26/0023A61L2400/04C08B37/0072
Inventor 孙春辉高力群
Owner CHANGZHOU SIYU ENVIRONMENTAL PROTECTION MATERIAL SCI & TECH
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