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Method for removing cyanide from rubber seeds through composite fermentation

A technology for compound fermentation and fermenting rubber, which is applied in the biological field to achieve the effects of good removal effect, increased fragrance and safety assurance.

Active Publication Date: 2016-03-23
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are few studies on the removal of cyanide by the compound fermentation of existing microorganisms. The purpose of the present invention is to provide a method for removing cyanide from rubber seeds by mixed fermentation of Pichia kuroshii and fusion Weissella

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the method for removing cyanide by mixing fermented rubber seeds of Pichia kuroshii and fusion Weissella, the specific operations are as follows:

[0014] (1) Inoculate 5% by volume of purified Pichia kruelii into 5mL LYPD liquid medium and culture on a shaker at 30°C for 18h until the number of effective viable bacteria is ≥10 10 CFU / mL; inoculate 5mL MRS liquid medium with 5% inoculum volume of the purified Weissella fused and preserved in cryopreservation, and culture on a shaker at 37°C for 16h until the number of effective viable bacteria is ≥10 10 CFU / mL;

[0015] (2) After mixing the activated Pichia kuruii and the fusion Weissella at a volume ratio of 1:1, inoculate the mixed strains in the fermentation medium at a ratio of 5% by volume, at 25 Under the condition of ℃, stand for fermentation and culture for 144 hours, wherein the fermentation medium is composed of water and pulverized rubber seeds passed through a 20-mesh sieve, the ratio of the t...

Embodiment 2

[0018] Embodiment 2: the method for removing cyanide of this composite fermented rubber seed, concrete operations are as follows:

[0019] (1) Inoculate 5% by volume of purified Pichia krusei into 5mL LYPD liquid medium and culture on a shaker at 32°C for 20h until the number of effective viable bacteria is ≥10 10 CFU / mL; Inoculate the fused Weissella fused with 5% inoculum in 5mL MRS liquid medium after purification and culture on a shaker at 35°C for 20h until the number of effective viable bacteria is ≥10 10 CFU / mL;

[0020] (2) After mixing the activated Pichia kurui and Weissella fusion at a volume ratio of 2:1, inoculate the mixed strains in the fermentation medium at a ratio of 8% by volume, at 30 Cultivate statically for 144 hours under the condition of ℃, in which the components of the fermentation medium are water and pulverized rubber seeds passed through a 40-mesh sieve, the ratio of the two is 1:2, and the fermented rubber seeds from which cyanide is removed are ...

Embodiment 3

[0023] Embodiment 3: the method for removing cyanide of this composite fermented rubber seed, concrete operations are as follows:

[0024] (1) Inoculate the purified and frozen Pichia krusei at a volume percentage of 5% in 5mL LYPD liquid medium for 24 hours on a shaker at 30°C until the number of effective viable bacteria is ≥10 10 CFU / mL; Inoculate the fused Weissella fused with 5% inoculum in 5mL MRS liquid medium after purification and culture in a shaker at 30°C for 24h until the number of effective viable bacteria is ≥10 10 CFU / mL;

[0025] (2) After mixing the activated Pichia kurui and Weissella fusion at a volume ratio of 1:2, inoculate the mixed strains in the fermentation medium at a ratio of 10% by volume, at 37 It is obtained by static culture at ℃ for 120 hours, in which the fermentation medium is water and rubber seed powder crushed to about 20 mesh and passed through a 60 mesh sieve, the ratio of the two is 2:1, and the fermented rubber from cyanide is obtaine...

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PUM

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Abstract

The invention discloses a method for removing cyanide from rubber seeds through composite fermentation. According to the method, the composite fermentation of Pichia kudriavzevii (Pichia kudriavzevii) and Weissella confusa (Weissella confusa) is adopted for removing the cyanide from the rubber seeds. The method comprises the following steps of separately performing activated culture on the Pichia kudriavzevii and the Weissella confusa until the effective viable count is greater than or equal to 1010CFU / mL, performing mixing so as to obtain a mixture, and adding the mixture to culture mediums which are obtained by mixing water and crushed rubber seed kernels for culture. Through research, researchers find that the composite fermentation is beneficial to cyanide removing effects, and the average cyanide removing rate is 88.9% or above. Lactic acid bacteria produce acid, so that the growth of infectious microbes can be restrained; fragrance is enhanced through the fermentation of yeast; therefore, under the premise that the unchanged quality of the rubber seeds is guaranteed, the cyanide is effectively removed.

Description

technical field [0001] The invention relates to a method for removing cyanide from rubber seeds compoundly fermented by Pichia kushori and Weissella fusion, belonging to the field of biotechnology. Background technique [0002] rubber( Hevea brasiliensis ) Euphorbiaceae trees, up to 30 meters high, rich in latex. Rubber tree is a typical tropical crop, native to Brazil; it is now widely cultivated in tropical Asia. It is cultivated in Hekou, Xishuangbanna and Ruili in Yunnan; it is cultivated in Guangdong, Guangxi, Hainan, southern Fujian and Taiwan. Capsule elliptic, 5-6 cm in diameter, with 3 longitudinal grooves, with a beak tip at the top, slightly concave at the base, thin exocarp, reticular veins at the bottom, thick endocarp, woody; seeds elliptic, light gray brown, There are markings. Rubber seeds are by-products of the rubber tree. For a long time in the past, a large number of rubber seeds have not been rationally and comprehensively utilized, and have been di...

Claims

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Application Information

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IPC IPC(8): A23K10/37A23K10/12C12N1/16C12N1/20C12R1/84C12R1/01
CPCC12N1/16C12N1/20Y02P60/87
Inventor 邓先余陈晗门文卉李先永林连兵郭军
Owner KUNMING UNIV OF SCI & TECH
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