Enzyme-free glucose electrochemical sensor electrode and preparation method and application thereof

A sensing electrode and electrochemical technology, which is applied in the fields of material electrochemical variables, scientific instruments, material analysis by electromagnetic means, etc., can solve the problems of high price, cumbersome preparation process, and high sensor cost, and achieve low cost and preparation technology. Simple, highly sensitive effects

Inactive Publication Date: 2016-03-23
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the good properties of nano-metal oxides, nano-metal oxides are also used in electrochemical sensors in the prior art, but they are basically noble metals and their oxide materials, which are expensive, the preparation process is cumbersome, and the cost of sensors is high.

Method used

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  • Enzyme-free glucose electrochemical sensor electrode and preparation method and application thereof
  • Enzyme-free glucose electrochemical sensor electrode and preparation method and application thereof
  • Enzyme-free glucose electrochemical sensor electrode and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] An enzyme-free glucose electrochemical sensor, the electrode used is Ni / MnO with composite structure 2 / Glassy carbon electrode, the specific preparation method of this electrode is as follows,

[0029] The glassy carbon electrode (GCE) is pretreated, and the surface of the glassy carbon electrode (GCE) is sequentially coated with γ-Al with a particle size of 1.0 μm and 0.05 μm 2 o 3 The powder was ground and polished, then ultrasonically washed with double distilled water or absolute ethanol for 10 minutes, and ventilated and dried;

[0030] Firstly, prepare the concentration of 6.0×10 -3 mol / L KMnO 4 solution and concentration of 4.0 x 10 -5 mol / L of H 2 SO 4 Solution, measure 50ml of each of the above two solutions and mix them thoroughly, then immerse the treated glassy carbon electrode at a set scanning speed of 0.002v / s, and scan 2 times in a voltage range of 0.3~-0.5V, and the obtained electrode labeled as MnO 2 / glassy carbon electrode;

[0031] Firstl...

Embodiment 2

[0034] An enzyme-free glucose electrochemical sensor, the electrode used is Ni / MnO with composite structure 2 The specific preparation method of the glassy carbon electrode is as follows,

[0035] The glassy carbon electrode (GCE) is pretreated, and the surface of the glassy carbon electrode (GCE) is sequentially coated with γ-Al with a particle size of 0.5 μm and 0.1 μm 2 o 3 The powder was ground and polished, then ultrasonically washed with twice distilled water or absolute ethanol for 20 minutes, and ventilated and dried;

[0036] Firstly, prepare the concentration of 1.0×10 -2 mol / L KMnO 4 solution and concentration of 5.0 x 10 -5 mol / L of H 2 SO 4 solution, measure 50ml with a concentration of 1.0×10 -2 mol / L KMnO 4 solution and 60ml concentration is 5.0×10 -5 mol / L of H 2 SO 4 solution, fully mix the two, and then immerse the treated glassy carbon electrode in the prepared mixed solution, set the scanning speed to 0.005v / s, and scan 2 times in the voltage ran...

Embodiment 3

[0039] In the specific measurement, the Ni / MnO prepared in Example 1 2 / Glassy carbon electrode as working electrode, saturated calomel electrode as reference electrode, platinum electrode as auxiliary electrode, connect to electrochemical workstation at the same time, and immerse the electrode in 4ml of 0.1mol / L NaOH solution, let it stand for 5min, and cycle Voltammetry scans 50-80 circles, the potential range is 0.1V-0.6V, and the scan speed is 0.1V / s to stabilize the electrodes.

[0040] First, cyclic voltammetry scans were performed, using bare electrodes, MnO 2 Electrodes and Ni / MnO 2 electrode, the result is as figure 2 Shown, Ni / MnO 2 The catalytic response of the electrode is the most obvious.

[0041] Simultaneously use the Ni / MnO that the embodiment of the present invention 1 makes 2 / GCE electrochemical sensing electrode obtained the CV curve and i-t curve of glucose, the specific results are as follows image 3 and Figure 4 shown. In the constant potenti...

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Abstract

The invention provides a preparation method of an enzyme-free glucose electrochemical sensor electrode. The method comprises the steps that gamma-Al2O3 powders are sequentially utilized to polish the surface of a glassy carbon electrode, and ultrasonic washing is conducted; the electrode is immersed into a certain concentration of mixed solution of KMnO4 and H2SO4, the scanning speed is set, two circles of cycle-scanning is conducted under the voltage range from 0.3 V to -0.5 V to prepare a MnO2/glassy carbon electrode; the electrode is put into a KCl solution containing a certain concentration of NiCl2, the pH value of the solution is adjusted to 7.00, the scanning speed is set, electro-deposition is conducted for 100-250 s under the voltage range from 0.9 V to -0.9 V, and the enzyme-free glucose electrochemical sensor electrode is obtained. According to the preparation method of the enzyme-free glucose electrochemical sensor electrode, common metal and metal oxide are adopted, the preparation technology is simple, the cost is low, the sensitivity is high, the stability is good, the service life is long, and the linearity range is wide. The preparation method of the enzyme-free glucose electrochemical sensor electrode is expected to be widely applied in the fields such as diabetes diagnosis, clinical medicine and food technology detection.

Description

technical field [0001] The invention relates to an enzyme-free glucose electrochemical sensor, in particular to an enzyme-free glucose electrochemical sensing electrode and its preparation method and application. Background technique [0002] Biosensor is a special kind of chemical sensor, which uses glucose enzyme as the biological sensitive element and is a highly selective detector for the measured target. It captures the reaction between the target substance and the sensitive element through various physical and chemical signal converters, and then expresses the degree of reaction with discrete or continuous electrical signals to obtain the concentration of the measured substance. [0003] Glucose is a very important compound. Human blood contains a certain concentration of glucose, referred to as blood sugar. Under normal circumstances, blood sugar maintains a dynamic balance. The sensing and detection of glucose has always been a hot issue in the research of electroche...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 王燕郑建斌
Owner NORTHWEST UNIV(CN)
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