Method for preparing double-resistant sandwich method test paper for ancient wool fabric

A technology of detection test paper and double-antibody sandwich, which is applied in the field of detection of traces of ancient wool fabrics, can solve the problems of difficult identification of wool products, and achieve the effects of intuitive results, high sensitivity and strong specificity

Inactive Publication Date: 2016-03-23
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the identification of silk cultural relics, this has caused certain difficulties in the identification of wool products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1 adopts the following steps:

[0012] A) prepare gold nanoparticle, adopt the method for sodium citrate reduction chloroauric acid to prepare the gold nanoparticle that particle diameter is 25nm; About 100ml mass concentration is that the chloroauric acid solution water bath of 0.01% is heated to boiling, adds 1.0ml rapidly The mass concentration is 1% sodium citrate solution, boils 7min, and solution presents red, promptly makes gold nano-sol;

[0013] B) Prepare gold-labeled rabbit anti-keratin antibody, use 0.1mol / l K 2 CO 3 Solution Adjust the pH of 100ml of colloidal gold solution to 9.2, add 20 μl of rabbit anti-keratin antibody with stirring, and the antibody concentration is 3.15mg / ml; then add 5ml of polyethylene glycol 20000 solution with a mass concentration of 1%, Stir at room temperature for 5 min, then centrifuge at 9000 r / min for 60 min, discard the supernatant; dissolve the precipitate in 0.01 mol / l pH of 8.2 tris buffer, i.e. Tris-HCl solu...

Embodiment 2

[0016] Embodiment 2 adopts the following steps:

[0017] A) prepare gold nanoparticles, adopt the method for sodium citrate reduction chloroauric acid to prepare the gold nanoparticles that particle diameter is 40nm; Be about to 100ml mass concentration be that the chloroauric acid solution water bath of 0.01% is heated to boiling, add 1.25ml rapidly The mass concentration is 1% sodium citrate solution, boils 8min, and solution presents red, promptly makes gold nano-sol;

[0018] B) Prepare gold-labeled rabbit anti-keratin antibody, use 0.1mol / l K 2 CO 3 Solution Adjust the pH of 100ml of colloidal gold solution to 9.2, add 35μl of rabbit anti-keratin antibody with stirring, and the antibody concentration is 3.15mg / ml; then add 5ml of 5% polyethylene glycol 20000 solution at room temperature Stir for 5min, then centrifuge at 0000r / min for 50min, discard the supernatant; dissolve the precipitate in 0.01mol / l pH of 8.2 tris buffer, i.e. Tris-HCl solution, the Tris -The mass c...

Embodiment 3

[0021] Embodiment 3 adopts the following steps:

[0022] A) prepare gold nanoparticles, adopt the method for sodium citrate reduction chloroauric acid to prepare the gold nanoparticles that particle diameter is 50nm; Be about to 100ml mass concentration be that the chloroauric acid solution water bath of 0.01% is heated to boiling, add 1.5ml rapidly The mass concentration is 1% sodium citrate solution, boils 10min, and solution presents red, promptly makes gold nano-sol;

[0023] B) Prepare gold-labeled rabbit anti-keratin antibody, use 0.1mol / l K 2 CO 3 Solution Adjust the pH of 100ml of colloidal gold solution to 9.2, add 50μl of rabbit anti-keratin antibody with stirring, and the antibody concentration is 3.15mg / ml; then add 5ml of 10% polyethylene glycol 20000 solution at room temperature Stir for 5min, then centrifuge at 11000r / min for 40min, discard the supernatant; dissolve the precipitate in 0.01mol / l pH of 8.2 tris buffer, i.e. Tris-HCl solution, the Tris -The mass...

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Abstract

The invention discloses a method for preparing double-resistant sandwich method test paper for ancient wool fabric. The method comprises the following steps that gold nano particles are prepared through a method for reducing chloroauric acid through sodium citrate; a Jinbiao rabbit anti-keratin antibody is prepared; test strips are assembled, a sample pad, a colloidal gold alloy pad, a nitrocellulose membrane and a water absorbing pad are sequentially assembled on a PVC bottom plate and cut into the test paper strips through a cutter, and the test strips are placed in an aluminum foil bag to be sealed and stored; 1 g of fabric trace sample is taken to be dissolved in 50-100 ml of Tris-HCl buffer liquid with the PH of 8.2, even stirring is carried out, a drop of supernate is taken to drop onto the sample pad of the assembled test strips after 2 h; after 5-10 min, a detecting line and a quality control line both display red, and it is indicated that keratin is included in the sample, and the fabric trace is the trace of the wool fabric. Compared with the prior art, the method is simple and fast, high in sensitivity, high in specificity, visual in result and more suitable for archaeology field analysis.

Description

technical field [0001] The invention belongs to the field of detection of traces of ancient woolen fabrics, and in particular relates to a preparation method of a double-antibody sandwich method detection test paper for rapidly detecting ancient woolen fabrics. Background technique [0002] In ancient times, woolen fabrics were widely used in clothing and blankets in the northern region, and woolen fabrics accounted for the vast majority. A large number of unearthed wool fabrics are as exquisite as the unearthed silk cultural relics. This is not only an important physical evidence of the evolution of ancient textile technology, but also has important reference value for the study of ancient social environment and human activities. However, there is a lack of in-depth research on the identification of wool keratin in ancient silk fabrics in the prior art. [0003] Wool keratin is a kind of hardened and insoluble protein, which contains both acidic groups including carboxyl g...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/68
CPCG01N33/531G01N33/6803
Inventor 胡智文苏孝鹏王秉
Owner ZHEJIANG SCI-TECH UNIV
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