Method for preparing D-mannoheptulose and persitol by extraction from avocado
A technology of mannoheptulose and avocado, which is applied in the field of preparation of D-mannoheptulose and avocado, can solve the problems of cumbersome process, unguaranteed purity, long production cycle, etc., and achieve easy industrialization , improve commercial value, and shorten the production cycle
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Embodiment 1
[0031] 1) Take the avocado fruit and blow it dry in an oven at 50°C, crush it into 30-40 meshes, and obtain avocado powder (detected by HPLC, wherein the content of D-mannoheptulose is 0.41%, and the content of avocado alcohol is 0.56%), standby;
[0032] 2) Take 300 g of avocado powder and place it in an extraction container, add 600 mL of 10% ethanol to it for ultrasonic extraction for 30 min, filter, add 600 mL of 10% ethanol to the filter residue for ultrasonic extraction for 30 min, and combine the filtrates to obtain an extract;
[0033] 3) The extract was concentrated under reduced pressure to a solid content of 6%, Angel yeast powder was added to the obtained concentrated solution, and fermented at room temperature for 24 hours, the addition of the baker's yeast was 1‰ of the weight of the concentrated solution;
[0034] 4) The obtained fermentation broth is centrifuged, the supernatant is collected and passed through a D101 macroporous resin column, eluted with deioni...
Embodiment 2
[0039] 1) adopt the raw material identical with embodiment 1 and obtain the extract of avocado by the method identical with embodiment 1;
[0040] 2) after the gained extract reclaims ethanol PCR642Ca ion-exchange resin (Purelight Co.), eluted with deionized water, collected the eluate in sections, and after HPLC analysis, combined the eluate rich in D-mannoheptulose to obtain eluate A; An eluent containing avocado to obtain eluent B;
[0041] 3) Concentrate the eluent A after being decolorized by activated carbon to obtain the concentrated solution A, add 95v / v% ethanol equivalent to 3 times its volume to it, place it at 15°C for crystallization, separate the crystals, dry them, and obtain the content 1.3 g of 71.2% D-mannoheptulose; concentrate the eluent B to obtain the concentrated solution B, add 95v / v% ethanol equivalent to twice its volume, crystallize at 15°C, and separate Crystals were obtained and dried to obtain 1.5 g of avocado with a content of 90.4%.
Embodiment 3
[0043] 1) Take the avocado fruit and freeze-dry it conventionally, crush it into 30-40 meshes, and obtain the avocado powder (detected by HPLC, wherein the content of D-mannoheptulose is 0.4%, and the content of avocado alcohol is 0.52%) ,spare;
[0044] 2) Take 500g of avocado powder and place it in an extraction container, add 2000mL of ethanol with a volume concentration of 50% to it for ultrasonic extraction for 30min, filter, add 2000mL of ethanol with a volume concentration of 50% for ultrasonic extraction for 30min to the filter residue, and combine the filtrates to obtain the extracted liquid;
[0045] 3) The extract was concentrated under reduced pressure to a solid content of 8%, Angel wine yeast was added to the obtained concentrated solution, and fermented at room temperature for 24 hours. The added amount of the fruit wine yeast was 1.5‰ of the weight of the concentrated solution;
[0046] 4) The obtained fermentation broth is centrifuged, and the supernatant is ...
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