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A kind of tissue culture rapid propagation method of American red maple

A technology of tissue culture rapid propagation and red maple, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as low germination rate and survival rate, low reproduction coefficient, long reproduction cycle, etc., to achieve The seedling propagation time is short, the cultivation process is simple, and the formula is simple

Active Publication Date: 2018-12-21
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly propagated by sowing and grafting, but the germination rate and survival rate are not very high, the reproduction coefficient is low, the reproduction cycle is long, the site requirements are large, and the labor cost is high, which is far from meeting the domestic demand for seedlings.

Method used

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  • A kind of tissue culture rapid propagation method of American red maple
  • A kind of tissue culture rapid propagation method of American red maple
  • A kind of tissue culture rapid propagation method of American red maple

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A kind of tissue culture rapid propagation method of American red maple described in the present embodiment, comprises the following steps:

[0031] (1) Selection and sterilization of explants: select young branches of American red maple, remove leaves, soak in washing liquid for 5-15 minutes, and then rinse in running water for 20-35 minutes; treated with alcohol for 25-40s, rinsed with sterile water for 2-4 times, then treated with 0.1% mercuric chloride for 4-8 minutes, rinsed with sterile water for 5-7 times, dried, and cut into 1-2cm in length with buds. The stem section is enough;

[0032] (2) Initiate culture: inoculate the explants treated in step (1) into an initiating medium with a pH value of 6.0 and cultivate them for 2-3 weeks to produce axillary buds. The initiating medium consists of MS basic medium , supplemented with 1.0mg / L PVP, 30g / L sucrose, 6g / L agar;

[0033] (3) Proliferation culture: cut out the axillary buds obtained in step (2), inoculate the...

Embodiment 2

[0039]A kind of tissue culture rapid propagation method of American red maple described in the present embodiment, the difference with embodiment 1 is:

[0040] In step (2), the composition of starting medium is: MS basic medium supplemented with 0.1mg / L PVP, 30g / L sucrose, 6g / L agar;

[0041] In step (3), the composition of the proliferation medium is: WPM basic medium, supplemented with 0.01mg / L 6-BA, 30g / L sucrose, 6g / L agar;

[0042] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 0.05 mg / L NAA, 20 g / L sucrose, and 6 g / L agar.

[0043] In this example, during the start-up culture process, the first axillary buds were generated for 8 days, and at the end of the start-up culture, the axillary buds elongated by about 2 cm; Certain callus, the time that the first cluster buds generate is 14 days, and when the proliferation culture ends, the cluster bud plant height is 1-4cm, and the multiplication coefficient is 1.7; , the averag...

Embodiment 3

[0045] A kind of tissue culture rapid propagation method of American red maple described in the present embodiment, the difference with embodiment 1 is:

[0046] In step (2), the composition of starting medium is: MS basic medium supplemented with 2.0mg / L PVP, 30g / L sucrose, 6g / L agar;

[0047] In step (3), the composition of the proliferation medium is: WPM basic medium, supplemented with 0.5mg / L 6-BA, 30g / L sucrose, 6g / L agar;

[0048] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 0.5 mg / L NAA, 20 g / L sucrose, and 6 g / L agar.

[0049] In this example, during the start-up culture process, the first axillary buds were formed on 9 days, and at the end of the start-up culture, the axillary buds elongated by 1-2 cm; Callus, the time when the first clustered shoots were generated was 14 days, and when the proliferation culture ended, the plant height of the clustered shoots was 1-4cm, and the proliferation coefficient was 2.33; in t...

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Abstract

The invention discloses a tissue culture rapid propagation method for acer rubrum. The tissue culture rapid propagation method includes the following steps of 1 explant selection and sterilization, wherein a stem segment, with buds, of acer rubrum are cut and sterilized with ethyl alcohol and mercury bichloride; 2 startup culture, wherein a startup culture medium is inoculated with an explant treated in the step 1, and the explant is cultured for 2-3 weeks so that axillary buds can be formed; 3 propagation culture, wherein the axillary buds obtained in the step 2 are cut, a propagation culture medium is inoculated with the axillary buds, and the axillary buds are cultured for 3-5 weeks so that cluster buds can be formed; 4, rooting culture, wherein the cluster buds obtained in the step 3 are separated into single plants, a rooting culture medium is inoculated with the cluster bud single plants, and the cluster bud single plants are cultured for 2-3 weeks so that sterile seedlings of acer rubrum can be obtained. The adopted culture media are simple in formula, the tissue culture flow path is simple and convenient, culture time is short, the proliferation frequency is high, the survival rate is large, the seedlings are uniform, uniform transplanting and planting management in the later period is facilitated, labor cost is reduced, shape uniformity of seedling plants grown out can be ensured, and the method is easy to operate and allows industrial production.

Description

technical field [0001] The invention relates to the technical field of biological group cultivation seedlings, in particular to a method for tissue culture and rapid propagation of American red maple. Background technique [0002] The American red maple, belonging to the family Aceraceae, is native to the northeast of the United States. It is a large tree with a round shape and is neat and graceful. It is the most popular greening tree species in the United States. In the past few years, since it was introduced into China, it has become the new darling of landscaping. Therefore, the research on American red maple has gradually increased. At present, it is mainly propagated by sowing and grafting, but the germination rate and survival rate are not very high, the reproduction coefficient is low, the reproduction cycle is long, the site requirements are large, and the labor cost is high, which is far from meeting the domestic demand for seedlings. [0003] In recent years, dom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李飞赵玲
Owner SICHUAN COLORLINK CO LTD