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Lactobacillus plantarum with function of relieving PFOA (Perfluorooctanoate) toxicity and application thereof

A technology of Lactobacillus plantarum and uses, applied in the field of microorganisms, can solve problems such as no development of toxic damage, and achieve the effect of good adsorption capacity

Active Publication Date: 2016-04-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the various physiological diseases caused by PFOA poisoning, the main research is currently on its toxicity mechanism, and no effective drugs have been developed to treat the toxic damage caused by it.

Method used

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  • Lactobacillus plantarum with function of relieving PFOA (Perfluorooctanoate) toxicity and application thereof
  • Lactobacillus plantarum with function of relieving PFOA (Perfluorooctanoate) toxicity and application thereof
  • Lactobacillus plantarum with function of relieving PFOA (Perfluorooctanoate) toxicity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1 : Tolerance of Lactobacillus plantarum CCFM738 to PFOA

[0104] The tolerance of Lactobacillus plantarum CCFM738 to PFOA was determined by the minimum inhibitory concentration (MIC) method. Prepare a 100mg / L PFOA solution as the mother liquor of PFOA, and filter it with a sterile filter with a pore size of 0.22 μm. Add the sterilized PFOA mother solution in proportion to the MRS agar medium and mix well so that the final concentrations are 1.0, 2.0, 5.0, 10, 20, 30, 50 mg / L, and pour the plates respectively. Divide each agar plate into four equal parts, add 2 drops of 10 μL Lactobacillus plantarum CCFM738 suspension to each part, make 2 parallel plates for each PFOA concentration, and use the agar plate without PFOA as the blank control. After incubation at 37°C for 48 h, the growth of the dropwise bacteria in each partition was recorded. The lowest PFOA concentration that inhibits the growth of the strain is the PFOAMIC of the strain. Thus, the MIC valu...

Embodiment 2

[0108] Example 2 : Experiment of adsorption capacity of Lactobacillus plantarum CCFM738 to PFOA

[0109] Incubate in PFOA-containing aqueous solution in vitro, and have good adsorption capacity for PFOA

[0110] Bacterial adsorption: Under aseptic conditions, according to the screening criteria of acid resistance (growth at pH 3.0), 10 strains of lactic acid bacteria were screened from traditional Chinese foods, such as kimchi and fermented milk wine. After purifying and activating the 10 strains of lactic acid bacteria, they were inoculated into MRS liquid medium according to 1% (v / v) inoculation amount, and cultivated at 37°C for 20h. Then centrifuge under the condition of 10000r / min for 20min, take the precipitate and wash it with ultrapure water, and then continue to centrifuge for 20min under the condition of 10000r / min, take the precipitate to obtain live bacterial cells, that is, wet bacterial cells. The wet bacteria were resuspended in 50 mg / LPFOA solution, and the fi...

Embodiment 3

[0117] Example 3 : Tolerance Dose Experiment of Lactobacillus plantarum CCFM738 Feeding Mice

[0118] The freeze-dried powder of Lactobacillus plantarum CCFM738 was resuspended in skim milk to make a concentration of 5.0×10 9 cfu / mL suspension. Take 10 healthy male C57BL / 6J mice of about 20-25g, orally administer the suspension of this concentration once a day, observe for a week, and record the death and body weight.

[0119] The results of these tests are listed in Appendix Table 3. These results indicated that feeding concentrations of 5.0×10 9 The cfu / mL Lactobacillus plantarum CCFM738 had no significant effect on the mice, the body weight increased significantly, and no death occurred. The appearance of the mice had no obvious pathological symptoms.

[0120] Table 3 Effect of feeding Lactobacillus plantarum CCFM738 on body weight and survival rate of mice

[0121]

[0122] Note: -: no death of mice

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Abstract

The invention discloses lactobacillus plantarum with a function of relieving PFOA (Perfluorooctanoate) toxicity and the application of the lactobacillus plantarum, and belongs to the technical field of microorganism. The lactobacillus plantarum CCFM738 with the function of relieving the PFOA toxicity, disclosed by the invention, has acid resistance, has good endurance capacity and good adsorption capacity on PFOA in vitro and can be used for improving blood indexes and liver oxidation indexes of a PFOA-exposed mouse; the lactobacillus plantarum CCFM738 is applied to a medicine composition with the function of relieving the PFOA toxicity and a leavening agent and has a very wide application prospect.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to a plant lactobacillus with the function of alleviating PFOA toxicity and its application, and the present invention also relates to the use of the plant lactobacillus. 【Background technique】 [0002] Perfluorooctanoic acid (Perfluorooctanoate, PFOA) is one of the most concerned perfluorinated organic acids in the world today. It has excellent chemical stability, thermal stability, and high hydrophobic and oleophobic properties. Under extremely low conditions, it can make fluorides have extremely high surface activity on its surface. It is an important raw material for the synthesis of polytetrafluoroethylene chemical products, and is widely used in industrial production and consumer goods for daily use. Due to its stable chemical properties, PFOA is difficult to be degraded in the environment, resulting in its long-term stable existenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P39/02C12R1/25
CPCA61K2035/115C12N1/205C12R2001/25
Inventor 陈卫王刚邢家溧田丰伟张秋香刘小鸣范大明赵国忠张白曦翟齐啸毛丙永郭敏赵建新张灏
Owner JIANGNAN UNIV
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