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Application of two-dimensional lysozyme nanometer film as antibacterial material

A technology of two-dimensional nanometer and antibacterial materials, which can be applied in application, fungicides, and chemicals for biological control. Spectrum and high-efficiency antibacterial performance, good antibacterial efficiency, and easy operation

Active Publication Date: 2016-04-13
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, as a water-soluble protein molecule, lysozyme has the common disadvantages of protein molecules, such as low mechanical strength, difficulty in forming a continuous film structure on a solid surface, poor stability, and rapid dissolution in aqueous solution, etc.
These characteristics limit its application to some extent

Method used

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  • Application of two-dimensional lysozyme nanometer film as antibacterial material
  • Application of two-dimensional lysozyme nanometer film as antibacterial material
  • Application of two-dimensional lysozyme nanometer film as antibacterial material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0025] Add 0.1433g of tris(2-carboxyethyl)phosphine to 10mL of 10mmol / L 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution with a pH of 7.4, adjust the pH to 6.4 with NaOH to prepare 50mmol / L of tris (2-Carboxyethyl) phosphine 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution; add 40mg of lysozyme into 10mL 10mmol / L 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution with a pH of 7.4 to prepare 4mg / mL 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution of lysozyme; take 10mL of 50mmol / L tris(2-carboxyethyl)phosphine 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution and 10mL4mg / mL lysis The 4-hydroxyethylpiperazine ethanesulfonic acid buffer solution of the enzyme is mixed uniformly and left at room temperature for 50 minutes to form a film on the surface of the mixed solution, that is, two-dimensional lysozyme nano-film, and ...

Embodiment 2

[0027] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0028] Add 0.1433g of tris(2-carboxyethyl)phosphine to 10mL of 10mmol / L tris(2-carboxyethyl) buffer solution with a pH of 7.4, adjust the pH to 6.4 with NaOH, and prepare 50mmol / L of tris(2-carboxyethyl) Tris buffer solution of phosphine; add 40mg of lysozyme to 10mL 10mmol / L tris buffer solution with a pH of 7.4 to prepare 4mg / mL tris of lysozyme Buffer solution; mix 10mL of 50mmol / L tris (2-carboxyethyl) phosphine tris (2-carboxyethyl) phosphine tris (2-carboxyethyl) phosphine buffer solution with 10mL 4mg / mL lysozyme tris buffer solution of tris (hydroxymethyl) aminomethane, mix well, insert the medical plastic catheter In the mixed liquid, let stand at room temperature for 50 minutes to form a self-assembled film directly on the inner and outer surfaces of the medical plastic catheter (see figure 1 ), that is, lysozyme two-dimensional nano film, which plays a role of ant...

Embodiment 3

[0030] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0031] The preparation method of the two-dimensional lysozyme nano-film in this example is the same as that in Example 1, and then the two-dimensional lysozyme nano-film is transferred to the surface of the surgical scalpel using agarose gel as a medium (see figure 2 ), play a role in antibacterial protection.

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Abstract

The invention discloses application of a two-dimensional lysozyme nanometer film as an antibacterial material. According to the invention, the two-dimensional lysozyme nanometer film is used as the antibacterial material and it only needs to grow the two-dimensional lysozyme nanometer film on the surface of a substrate in situ during preparation of the two-dimensional lysozyme nanometer film or directly adhering the two-dimensional lysozyme nanometer film onto the surface of the substrate, and the two-dimensional lysozyme nanometer film can grow on the surfaces of substrates of different shapes in situ or adhere onto the surfaces of substrates of different shapes. As the antibacterial material, the two-dimensional lysozyme nanometer film has broad-spectrum and high-efficiency antibacterial properties, has a broadened spectrum of resisting bacteria including Gram-positive bacteria, Gram-negative bacteria and fungi compared with conventional classical Gram-positive bacteria and shows good antibacterial efficiency to the Gram-positive bacteria, Gram-negative bacteria and fungi, wherein antibacterial efficiency of the Gram-positive bacteria, Gram-negative bacteria and fungi all reach 90% or more within 2 h; the two-dimensional lysozyme nanometer film has good adhesion on a protected substrate and no toxicity and adsorptivity to cells, so the two-dimensional lysozyme nanometer film has good anti-fouling property to cells; and the two-dimensional lysozyme nanometer film can be used as an ideal antibacterial food packaging material and a medical application program material.

Description

Technical field [0001] The invention belongs to the technical field of antibacterial materials, and specifically relates to the application of a lysozyme two-dimensional nano film as an antibacterial material. Background technique [0002] In various fields including packaging, textile industry, medicine, water purification and health care, it is very important to impart an antibacterial property. Composite materials with antibacterial properties are widely used due to their excellent comprehensive properties. This composite antibacterial material can assemble different types and functions of substances according to different needs. These characteristics give the composite material more and newer functions. However, composite antibacterial materials also have their own disadvantages. First, the monomer used to prepare the composite antibacterial material has different chemical stability relative to the surrounding media, which will lead to the unstable structure of the composite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/34A01P1/00A01P3/00
CPCA01N25/34A01N63/00
Inventor 杨鹏古金
Owner SHAANXI NORMAL UNIV
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