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Method for rapidly breeding hairy roots of vietnamese sophora roots

A hairy root and root propagation technology, applied in the field of plant biology, can solve the problems of rising prices of medicinal materials and the depletion of wild resources, and achieve the effects of fast growth, great application prospects, and stable synthesis ability

Inactive Publication Date: 2016-04-20
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the increase in the amount of medicines and the expansion of the way of use, the market demand for mountain bean root is constantly increasing, and the price of medicinal materials is also rising, which leads to the uncontrolled mining of mountain bean root resources, and the wild resources are on the verge of exhaustion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] An example of the method for rapid propagation of the hairy root of Radix japonica of the present invention, comprises the following steps:

[0015] (1) Obtainment of sterile seedlings: take the seeds of the rhizome of rhizome rhizome and place them in 0.1v / v% mercuric chloride for disinfection for 10 minutes, then rinse with sterile water for 3 times, and remove the surface moisture with sterile filter paper, and then inoculate into 1 without any hormones. / 2MS medium, placed in a culture room for germination, to obtain aseptic seedlings of soybean root, wherein 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8;

[0016] (2) R1601 Agrobacterium rhizogenes induces aseptic cotyledon hairy roots of Radix rhizogenes: put the aseptic cotyledons of Radix rhizogenes in R1601 bacterial solution for 16min and take out, take sterile water as the control, and then absorb with sterile filter paper The excess bacterial liquid on the s...

Embodiment 2

[0019] Another example of the method for rapid propagation of the hairy root of C. japonica of the present invention, comprises the following steps:

[0020] (1) Obtainment of sterile seedlings: take the seeds of the rhizome of rhizome rhizome and place them in 0.1v / v% mercuric chloride for disinfection for 10 minutes, then rinse with sterile water for 3 times, and remove the surface moisture with sterile filter paper, and then inoculate into 1 without any hormones. / 2MS medium, placed in a culture room for germination to obtain cotyledons of aseptic seedlings of Radix japonica, wherein 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8;

[0021] (2) R1601 Agrobacterium rhizogenes induces aseptic cotyledon hairy roots of Radix rhizogenes: put the aseptic cotyledons of Radix rhizogenes in R1601 bacterial solution for 16min and take out, take sterile water as the control, and then absorb with sterile filter paper The excess bacteria...

Embodiment 3

[0024] An example of the method for rapid propagation of the hairy root of Radix japonica of the present invention, comprises the following steps:

[0025] (1) Obtainment of sterile seedlings: take the seeds of the rhizome of rhizome rhizome and place them in 0.1v / v% mercuric chloride for disinfection for 10 minutes, then rinse with sterile water for 3 times, and remove the surface moisture with sterile filter paper, and then inoculate into 1 without any hormones. / 2MS medium, placed in a culture room for germination to obtain cotyledons of aseptic seedlings of Radix japonica, wherein 30g / L sucrose and 3.4g / L agar were added to the MS medium, and the pH value of the medium was 5.8;

[0026] (2) R1601 Agrobacterium rhizogenes induces aseptic cotyledon hairy roots of Radix rhizogenes: put the aseptic cotyledons of Radix rhizogenes in R1601 bacterial solution for 16min and take out, take sterile water as the control, and then absorb with sterile filter paper The excess bacterial ...

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Abstract

The invention provides a method for rapidly breeding hairy roots of vietnamese sophora roots. The method comprises the following steps: (1) seeds of Sophora tonkinensis are disinfected and inoculated to a 1 / 2 MS medium for germination, and aseptic seedlings of Sophora tonkinensis are obtained; (2) R1601 Rhizobium rhizogenes is used for inducing rooting of aseptic cotyledons of Sophora tonkinensis; (3) division of hairy roots obtained in the step (2) is carried out, and the divided hairy roots are placed in a nutrient solution for rapid breeding and cultivation, and the breeding multiple reaches 39-55 times. The hairy roots of vietnamese sophora roots have the characteristics of fast growth speed, good genetic stability, and good and stable synthesis capability; partial metabolites are released to the medium, and the hairy roots can be used as alternative resources of industrial vietnamese sophora roots in large scale production.

Description

technical field [0001] The invention relates to a method for plant biotechnology, in particular to a method for rapid propagation of hairy roots of bean root. Background technique [0002] Mountain bean root, also known as broad bean root and southern bean root, is the dry root and rhizome of the leguminous plant Sophoratonkinensis Gapnep., distributed in southern my country, with the largest yield in Guangxi. It has the functions of clearing fire and detoxifying, reducing swelling and relieving pain, and can treat laryngeal carbuncle, laryngeal wind, throat paralysis, swollen gums, dyspnea and hot cough, hepatitis, constipation, jaundice, diarrhea, hemorrhoids, alopecia, scabies, mosquitoes and insects. , dog bites, etc. The pharmaceutical industry uses rhizome root as a raw material to develop a large number of injections for the treatment of hepatitis, tablets for sore throat and anti-tumor Chinese patent medicines. At the same time, it is also used in the industry to ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/008
Inventor 李林轩韦坤华韦范缪剑华李翠韦莹王一诺肖东
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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