New application of p-hydroxybenzyl alcoholohol
A technology of p-hydroxybenzyl alcohol and uses, applied in the directions of active ingredients of hydroxy compounds, metabolic diseases, cardiovascular system diseases, etc., to achieve the effect of obvious effect, good market prospect and broad application space
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experiment example 1
[0035] Experimental Example 1 Effect of p-Hydroxybenzyl Alcohol of the present invention on blood lipids of high-fat diet ApoE- / -mice
[0036] (1) Experimental materials
[0037] Experimental drugs and reagents:
[0038] p-Hydroxybenzyl alcohol;
[0039] NaCl, analytically pure, Xilong Chemical Co., Ltd., batch number: 120808;
[0040] NaH2PO4·2H2O, analytically pure, Xilong Chemical Co., Ltd., batch number: 110108;
[0041] NaHCO3, analytically pure, Xilong Chemical Co., Ltd., batch number: 120721;
[0042] Glucose, analytically pure, Xilong Chemical Co., Ltd., batch number: 130305;
[0043] MgSO4 7H2O, analytically pure, Xilong Chemical Co., Ltd., batch number: 101028;
[0044] KCl, analytically pure, Xilong Chemical Co., Ltd., batch number: 110301;
[0045] CaCl2, analytically pure, Xilong Chemical Co., Ltd., batch number: 100314;
[0046] HEPES, number: H8090, solarbio company;
[0047] Triglyceride detection kit, Zhongsheng Beikong Biotechnology Co., Ltd.;
[004...
experiment example 2
[0061]Experimental Example 2 Effect of p-Hydroxybenzyl Alcohol of the present invention on vascular function of high-fat diet ApoE- / -mice
[0062] (1) Experimental materials
[0063] p-Hydroxybenzyl alcohol; mouse aorta obtained in Experimental Example 1.
[0064] (2) Experimental method
[0065] A. Measurement of Vascular Tonometry
[0066] The thoracic aorta obtained from the experiment was completely put into the pre-prepared ice Krebs solution, and the perivascular fat was carefully removed under a microscope. Cut the fat-removed blood vessels into small rings with a length of 3-4mm. Be careful not to tear or damage the inner wall of the blood vessels, and then hang the blood vessel ring device carefully and gently. The blood vessels were immersed in oxygenated Krebs solution at 37°C, and the equilibrium was started. The equilibrium condition was a tension of 1.5g, and the time interval was 10min. When the vascular tension is at 1.5g, add phenylephrine (PE) at a final ...
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