Composite bioactive factor freeze-drying method and composite bioactive factor freeze-dried powder

A bioactive factor and freeze-dried powder technology, which is applied in freeze-dried transportation, powder transportation, drug combination, etc., can solve unfavorable freeze-dried storage of composite bioactive factors, limit the use of composite bioactive factors, and reduce the activity of composite bioactive factors To achieve the effect of delaying skin aging, improving storage conditions, and reasonable cost

Inactive Publication Date: 2016-04-27
SHAANXI AMY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there are some defects in the freeze-drying methods disclosed in the prior art, which are unfavorable for the freeze-drying preservation of complex biologically active factors. For example, in the patent with the publication number CN101444619A, Tween series are used. Limit the later use of complex bioactive factor freeze-dried powder; and phosphate buffer is added during the freeze-drying process, which contains a large amount of salt, which will reduce the activity of complex bioactive factors
In the patent with the publication number CN103251649A, the liquid needs to be frozen and solidified at -80°C before sublimation drying, which consumes a lot of energy and leads to an increase in cost; and the obtained freeze-dried powder needs to be stored at -20°C, and the storage conditions are very harsh, which is not conducive to preservation and transportation

Method used

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  • Composite bioactive factor freeze-drying method and composite bioactive factor freeze-dried powder
  • Composite bioactive factor freeze-drying method and composite bioactive factor freeze-dried powder
  • Composite bioactive factor freeze-drying method and composite bioactive factor freeze-dried powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Take 10 parts of mannitol and mix with 10 parts of compound bioactive factor solution, and add purified water to 100 parts to obtain a mixed solution;

[0089] The mixed solution is sequentially subjected to pre-freezing, enhanced pre-freezing, sublimation drying and analytical drying (the specific process is the same as described above) to obtain the freeze-dried powder of the complex biologically active factor.

[0090] In conclusion, the obtained composite bioactive factor lyophilized powder comprises 10 parts of mannitol, 0.01 part of composite bioactive factor, and the composite bioactive factor keeps the activity of more than 75% of the bioactive factor in the composite bioactive factor solution in the gained freeze-dried powder, mainly are EGF, BFGF, VEGF, IGF1, IGF2, TGF-B1, TGF-B2, PDGF and KGF.

[0091] The obtained composite bioactive factor freeze-dried powder can be stored for 2 years at 2-8°C, and can be stored for 1-2 years at room temperature.

Embodiment 2

[0093] Mix 5 parts of sorbitol with 5 parts of complex bioactive factor solution, and add purified water to 100 parts to obtain a mixed solution;

[0094] The mixed solution is sequentially subjected to pre-freezing, enhanced pre-freezing, sublimation drying and analytical drying (the specific process is the same as described above) to obtain the freeze-dried powder of the complex biologically active factor.

[0095] Conclusion, gained compound bioactive factor freeze-dried powder comprises 5 parts of sorbitol, 0.005 part of compound biologically active factor, compound biologically active factor keeps the activity of more than 72% of the biologically active factor in the compound biologically active factor solution in the gained freeze-dried powder, mainly are EGF, BFGF, VEGF, IGF1, IGF2, TGF-B1, TGF-B2, PDGF and KGF.

[0096] The obtained composite bioactive factor freeze-dried powder can be stored at 2-8°C for 2 years, and stored at room temperature for 1-2 years.

Embodiment 3

[0098] Take 8 parts of the mixture of sorbitol and mannitol (the specific ratio can be arbitrary), 0.5 part of trehalose, 0.6 part of dextran 40, mix with 5 parts of the compound biologically active factor solution, and add purified water to 100 parts to obtain a mixed solution ;

[0099] The mixed solution is sequentially subjected to pre-freezing, enhanced pre-freezing, sublimation drying and analytical drying (the specific process is the same as described above) to obtain the freeze-dried powder of the complex biologically active factor.

[0100] In conclusion, the obtained composite bioactive factor lyophilized powder contains 8 parts of sorbitol and mannitol, 0.5 part of trehalose, 0.6 part of dextran 40, and 0.005 part of the composite bioactive factor, and the composite bioactive factor in the obtained freeze-dried powder maintains the composite biological activity More than 78% of the biologically active factors in the factor solution are mainly EGF, BFGF, VEGF, IGF1, ...

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Abstract

The invention relates to the technical field of biological tissue culture, in particular to a composite bioactive factor freeze-drying method and composite bioactive factor freeze-dried powder. Bioactivity and biological stability of the composite bioactive factor freeze-dried powder are improved by the freeze-drying method; the produced composite bioactive factor freeze-fried powder can be refrigerated or preserved at normal temperature for a long time. According to an embodiment of the invention, the composite bioactive factor freeze-drying method includes subjecting a liquid mixture of protectant and 5-10 parts of solution containing composite bioactive factors to pre-freezing, intensified pre-freezing, drying by sublimation and drying by desorption sequentially. The protectant comprises 5-10 parts of polyatomic alcohol, and the liquid mixture particularly comprises the protectant, the solution containing composite bioactive factors and the balance being purified water.

Description

technical field [0001] The invention relates to the technical field of biological tissue culture, in particular to a freeze-drying method and freeze-dried powder of complex biologically active factors. Background technique [0002] Bioactive factors are produced by various cell types in a paracrine, autocrine or endocrine manner, and can regulate cell growth, differentiation, and immune functions, and can especially effectively interact with skin cells to participate in inflammation and wound healing. For example: bioactive factors can promote the nutrient metabolism of epithelial cells and prevent the skin from being damaged by ultraviolet rays and free radicals; bioactive factors can also promote the proliferation of collagen cells in the dermis and accelerate postoperative skin repair. [0003] Common bioactive factors secreted by cells include EGF (Chinese: epidermal growth factor, English: epidermal growth factor), BFGF (Chinese: basic fibroblast growth factor, English:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K9/19A61K47/10A61P17/02A61P17/18
Inventor 张青刘莹南艳萍张琪张爱兵
Owner SHAANXI AMY BIOTECH CO LTD
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