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Porcine parvovirus-porcine pseudorabies combined inactivated vaccine, and preparation method and application thereof
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A dual inactivated vaccine, porcine pseudorabies technology, applied in the directions of biochemical equipment and methods, vaccines, viruses, etc., can solve the problems of complex immunization procedures and high costs, and achieve optimized immunization procedures, good safety, and good safety. Effect
Inactive Publication Date: 2016-04-27
哈药集团生物疫苗有限公司
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Problems solved by technology
At present, there are only single vaccines for porcine parvovirus and porcine pseudorabies in the Chinese market. To prevent these two diseases, PPV and PRV single vaccines need to be inoculated separately. The immunization procedure is complicated and the cost is high.
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Embodiment 1
[0032] Preparation and inspection of the dual inactivated vaccine of porcine parvovirus disease-porcine pseudorabies of embodiment 1
[0033] 1.1 Preparation of poisonous seeds for production
[0034] Preparation of PPVL strain: Dilute the virus seed appropriately with the virus diluent (serum-free MEM medium), inoculate it into a single-layer ST cell culture at a multiplicity of infection (M.O.I.) of 0.01, and place it in a 37°C rotary culture. When the lesion reaches 80%, harvest the toxic cell culture medium, freeze and thaw 3 times, store at -20°C, and the virus titer should be ≥10 6.17 TCID 50 / ml.
[0035] Preparation of PRV-JL strain: Dilute the virus seeds appropriately with virus diluent (serum-free MEM medium), and inoculate them in ST cell cultures that form a monolayer of 40% to 60% according to the multiplicity of infection (M.O.I.) of 0.01 , placed at 37°C for rotary culture, when the lesion reached 80%, harvested the toxic cell culture medium, frozen and thaw...
Embodiment 2
[0096] The dual inactivated vaccine prepared by the different proportions of the vaccine of embodiment 2PPV, PRV is compared with each single vaccine (PPV inactivated vaccine and PRV inactivated vaccine) immune effect
[0097] 1. Vaccines
[0098] PPV, PRV double inactivated vaccine, select the laboratory product among the embodiment 1 (batch number is 2014001, PPV: PRV=2:3) and according to the method for embodiment 1, porcine parvovirus antigen, porcine pseudorabies virus antigen are respectively PPV by volume ratio: PRV=5:1, 4:1, 3:1, 5:2, 2:1, 3:2, 1:1, 3:4, 1:2, 2:5, 1:3 , 1:4, 1:5 prepared double vaccine; PPV disease inactivated vaccine single vaccine (batch number 201406); PRV inactivated vaccine single vaccine (batch number 201407).
[0101] Adult guinea pigs with a body weight of 350 g or more and an HI antibodytiter ≤ 1:8 were randomly divided into 16 groups, with 20 guinea pigs in each gro...
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Abstract
The invention discloses a porcine parvovirus-porcine pseudorabies combined inactivated vaccine, and a preparation method and an application thereof. The preparation method comprises the following steps: respectively inactivating a porcine parvovirus liquid and a porcine pseudorabies liquid with pyrrole to obtain a porcine parvovirusantigen and a porcine pseudorabiesantigen, and mixing the porcine parvovirusantigen and the porcine pseudorabies antigen with an oil phaseadjuvant to prepare the combined inactivated vaccine. No immunosuppression exists between the two antigen components in the combined inactivated vaccine, the combined inactivated vaccine prepared from the porcine parvovirus antigen and the porcine pseudorabies antigen according to a volume ratio of 2:3 has better safety and immunoprotection effects than single vaccines, and the porcine parvovirus and the porcine pseudorabies can be substantially immunoprotected through one-shot syringe injection. The porcine parvovirus-porcine pseudorabies combined inactivated vaccine has the advantages of good safety, avoiding of untoward effects of multi-time immunoprophylaxis, reduction of the immunization cost, and use convenience, and can be applied in effective prevention of the porcine parvovirus and the porcine pseudorabies.
Description
technical field [0001] The invention relates to porcine parvovirus-porcine pseudorabies dual inactivated vaccine, and also relates to a preparation method of the dual inactivated vaccine and its application in preventing porcine parvovirus and porcine pseudorabies, belonging to porcine parvovirus 1. The field of preparation of porcine pseudorabies dual inactivated vaccine. Background technique [0002] Both porcine parvovims (PPV) and porcine pseudorabies virus (Pseudorabiesvires, PRV) have long been shown to cause reproductive disorders in sows. After the sow is infected, the clinical symptoms are similar, all of which are abortion, stillbirth, mummified fetus, and repeated infertility in sows. Porcine parvovirus and porcine pseudorabies virus are distributed worldwide, causing huge and continuous economic losses to the global pig industry. China first discovered porcine parvovirus infection in Shanghai in 1983, and carried out virus isolation and identification. In 1947,...
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