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Cultivation method of borneol cinnamomum comphora with high borneol content

A cultivation method, technology of borneol camphor, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of reducing borneol content, low regeneration rate, long cycle, etc., to increase light intensity and reduce stimulation Effect

Inactive Publication Date: 2016-05-04
文沁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under these conditions, the regeneration efficiency of adventitious buds in explants is very low, and the quality of buds is also poor, reducing the content of borneol
At the same time, these regeneration systems all have the problems of long cycle and low regeneration rate, which seriously restrict the research and application of high borneol content.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for cultivating borneol camphor camphor with high borneol content, comprising:

[0029] (1) Selection and disinfection of explants: choose the germination of a single plant of borneol camphor with high borneol content as an explant, soak it in a beaker filled with saturated soapy water for 5 minutes, and then rinse it with tap water to the basic If there is no foam, then repeat the above soaking and rinsing steps once; soak in 75% ethanol for 1 minute in the ultra-clean bench, rinse with sterile water twice, and disinfect with 5% sodium hypochlorite solution containing two drops of Tween 80 for 10 minutes, without Rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride containing two drops of Tween 80 for 10 minutes, and rinse with sterile water for 5 times;

[0030] (2) soaking treatment: soak the explant described in step (1) with 4mg / L isopentenyl adenine solution for 20min;

[0031] (3) Inoculation culture: inoculate the explants t...

Embodiment 2

[0036] A method for cultivating borneol camphor camphor with high borneol content, comprising:

[0037] (1) Selection and disinfection of explants: choose the germination of a single plant of borneol camphor with high borneol content as explants, soak in a beaker filled with saturated soapy water for 10 minutes, then rinse with tap water to the basic If there is no foam, repeat the above soaking and rinsing steps once again; soak in 75% ethanol for 1 minute in the ultra-clean bench, rinse with sterile water twice, and sterilize with 5% sodium hypochlorite solution containing two drops of Tween 80 for 15 minutes. Rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride containing two drops of Tween 80 for 15 minutes, and rinse with sterile water for 5 times;

[0038] (2) Soaking treatment: soaking the explant described in step (1) with 6mg / L isopentenyl adenine solution for 40min;

[0039] (3) Inoculation culture: the explants treated in step (2) are in...

Embodiment 3

[0044] A method for cultivating borneol camphor camphor with high borneol content, comprising:

[0045] (1) Selection and disinfection of explants: choose the germination of a single plant of borneol camphor with high borneol content as explants, soak in a beaker filled with saturated soapy water for 8 minutes, then rinse with tap water to the basic If there is no foam, repeat the above soaking and rinsing steps once again; soak in 75% ethanol for 1 minute in the ultra-clean bench, rinse with sterile water twice, and sterilize with 5% sodium hypochlorite solution containing two drops of Tween 80 for 12 minutes. Rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride containing two drops of Tween 80 for 13 minutes, and rinse with sterile water for 5 times;

[0046] (2) Soaking treatment: soaking the explant described in step (1) with 5mg / L isopentenyl adenine solution for 30min;

[0047] (3) Inoculation culture: the explants treated in step (2) are ino...

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Abstract

The invention relates to a cultivation method of borneol cinnamomum comphora with high borneol content, and belongs to the field of plant tissue culture. The cultivation method comprises the following steps: (1) selection and sterilization of an explant; (2) soaking treatment; (3) inoculation culture; (4) propagation culture; (5) rooting culture; (6) seedling hardening and transplantation. According to the cultivation method of the borneol cinnamomum comphora with the high borneol content, disclosed by the invention, plant hormones of an induction medium can be removed; before inoculation, the soaking treatment is carried out on the explant by adopting an isopentenyl adenine solution; during a subculture process, the illumination intensity is increased, the photoperiod is prolonged, and the culture temperature is reduced; meanwhile, the excellent effects of quick propagation rate, stable hereditary features and high borneol content are achieved by adopting a technological means of converting a subculture medium.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a method for cultivating borneol camphor with high borneol content. Background technique [0002] Camphor tree (Cinnamomum comphora) is an evergreen tree species of the genus Cinnamon in the family Lauraceae, and is a native species in Ji'an City, Jiangxi Province. Camphor trees are divided into borneol camphor, camphor camphor, camphor camphor, oil camphor, different camphor and other types. Among the above types, borneol camphor is the tree species with the least natural distribution and the highest economic value. The natural d-borneol extracted through the plantation of borneol camphor coppice is widely used in industries such as medicine, flavors and fragrances, and cosmetics, and has broad market prospects. For a long time, the development and utilization of borneol camphor has been mainly based on the extraction of essential oils. Over-harvesting has caused serious damage to it...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
CPCA01G24/00A01G31/00A01H4/008
Inventor 文沁
Owner 文沁
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