Method for separation of Chinese cabbage microspores at different development stages
A developmental stage, microspore technology, applied in the field of plant biology, can solve problems such as the difficulty of consistent specific standards, the difficulty of building a Chinese cabbage regeneration system, and the difficulty of ensuring repeatability.
Inactive Publication Date: 2016-05-04
SHENYANG AGRI UNIV
2 Cites 2 Cited by
AI-Extracted Technical Summary
Problems solved by technology
The invention discloses a method for separation of Chinese cabbage microspores at different development stages. The method includes: preparing a percoll separation liquid, placing a Chinese cabbage microspore crude extracted solution to the top, carrying out 150rpm low speed centrifugation to separate microspore at different development stages. Microspores at different development stages have close relation with the formation of vacuoles and other organelles, i.e. usually showing the difference of relative density. Therefore, separation of microspores at different development stages can be achieved by percoll density gradient centrifugation. The method provided by the invention can efficiently and stably separate microspores at different development stages, overcomes the disadvantages of difficult grasp of petal length/anther length ratio, flower bud length and the like in experiments utilizing traditional morphological indices, solves the problem of in-vitro preparation of microspore cells at similar development stages, and can provide materials for the mechanism study of Chinese cabbage microspore culture and provide receptors for gene gun mediated Chinese cabbage genetic transformation.
- Experimental program(1)
Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.