Screening and application of fumonisin B1 aptamer strand displacement probe
A nucleic acid aptamer and fumonisin technology, which is applied in biochemical equipment and methods, material inspection products, biological testing, etc., can solve the problems of few application reports of nucleic acid aptamers, achieve low synthesis cost, reduce costs, good reproducibility
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[0023] a fumonisin B 1 The nucleic acid aptamer strand displacement probe is a single-stranded oligonucleotide whose sequence is shown in SEQ ID NO:1.
[0024] The 5' end of the probe is modified with a FAM fluorescent group.
[0025] The probe and fumonisin B 1 Aptamers are complementary.
[0026] The fumonisin B 1 The nucleic acid aptamer sequence is shown in SEQ ID NO:6.
[0027] The fumonisin B 1 Aptamer strand displacement probes were screened by fluorescence spectroscopy and fluorescence polarization.
[0028] Fumonisin B 1 Nucleic Aptamer Strand Displacement Probe as Fumonisin B in Maize and Wheat 1 Fluorescence polarization detection analysis applications.
[0029] Below in conjunction with specific embodiment, the present invention is described in detail, and concrete steps are as follows:
[0030] FB used in the present invention 1 The nucleic acid aptamer sequence is FB reported by MaureenMcKeague in 2010 1 The sequence of the nucleic acid aptamer is show...
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