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A preparation method of enzymolysis rice protein with increased telomere length

A technology of rice protein and telomere length, which is applied in the field of enzymolysis of rice protein preparation, can solve the problems of large amount of enzyme, high production cost, and low efficiency of enzymatic hydrolysis

Active Publication Date: 2022-01-14
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the products of microbial fermentation are macromolecular peptides, while enzymatic hydrolysis also has problems such as low enzymatic hydrolysis efficiency, large enzyme dosage, and high production costs.

Method used

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  • A preparation method of enzymolysis rice protein with increased telomere length
  • A preparation method of enzymolysis rice protein with increased telomere length

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Experimental program
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Effect test

Embodiment 1

[0040] A preparation method for enzymatically hydrolyzed rice protein with increased telomere length, using the following steps:

[0041] 1) After the rice is pulverized and pretreated by filtration, add water and stir evenly to make a fermentation stock solution with a material-to-liquid ratio of 1:80 g / L;

[0042] 2) Put the fermentation liquid in a pressure cooker at 100 o Cook at a temperature of C for 30 min;

[0043] 3) Cool down the fermented stock solution after cooking to 60 o After C, insert the Bacillus subtilis of raw material ratio 10%, maintain fermentation temperature and be 60 o C, the fermentation humidity is 80%, the fermentation time is 64 h, after the fermentation is finished, the filtrate is filtered and used for later use;

[0044] 4) Put the fermentation filtrate obtained in step 3) into an ultrasonic tank for ultrasonic treatment at a frequency of 100 KHZ, and then add modified trypsin: modified papain: PEG-100 stearate at a mass ratio of 1:1:1 The ...

Embodiment 2

[0048] A preparation method for enzymatically hydrolyzed rice protein with increased telomere length, using the following steps:

[0049] 1) After the rice is pulverized and pretreated by filtration, add water and stir evenly to make a fermentation stock solution with a material-to-liquid ratio of 1:1g / L;

[0050] 2) Put the fermentation liquid in a pressure cooker at 60 o Cook at a temperature of C for 60 min;

[0051] 3) Cool down the fermented stock solution after cooking to 40 o After C, insert the Bacillus subtilis of raw material ratio 0.1%, maintain fermentation temperature to be 40 o C, the fermentation humidity is 50%, the fermentation time is 24 h, after the fermentation is finished, the filtrate is filtered and used for later use;

[0052] 4) Put the fermentation filtrate obtained in step 3) into an ultrasonic tank for ultrasonic treatment at a frequency of 5 KHZ, and then add modified trypsin: modified papain: PEG-100 stearate at a mass ratio of 1:10:10 The com...

Embodiment 3

[0056] A preparation method for enzymatically hydrolyzed rice protein with increased telomere length, using the following steps:

[0057] 1) After the rice is pulverized and pretreated by filtration, add water and stir evenly to make a fermentation stock solution with a material-to-liquid ratio of 1:50g / L;

[0058] 2) Put the fermentation liquid in a pressure cooker at 75 o Cook at a temperature of C for 45 min;

[0059] 3) Cool down the fermented stock solution after cooking to 45 o After C, insert the Bacillus subtilis of raw material ratio 5%, maintain fermentation temperature and be 45 o C, the fermentation humidity is 70%, the fermentation time is 36 h, after the fermentation is finished, filter and get the filtrate for later use;

[0060] 4) Put the fermentation filtrate obtained in step 3) into an ultrasonic tank for ultrasonic treatment at a frequency of 50 KHZ, and then add modified trypsin: modified papain: PEG-100 stearate at a mass ratio of 1:5:3 The compound p...

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Abstract

The invention relates to a preparation method of enzymatically hydrolyzed rice protein with increased telomere length, which is characterized in that: the following steps are adopted: 1) the rice is pulverized, filtered and pretreated, then added with water and stirred evenly; 2) put in a pressure cooker 60~120 o Cook at a temperature of C for 30~60 min; 3) Cool down to 40~60 o C, adding Bacillus subtilis that accounts for 0.1% to 10% of the mass fraction of the mixture is fermented, and the fermentation temperature is 40 to 60 o C. The fermentation humidity is 50%~90%, and the fermentation time is 24~72 h. After the fermentation, the fermentation filtrate is obtained for use; 4) The fermentation filtrate is placed in an ultrasonic tank for ultrasonic treatment at a frequency of 5~100KHZ, and then Compound protease solution to form enzymatic hydrolysis solution, 5) adjust the pH of the enzymatic hydrolysis solution to 6.0~8.0, and the temperature to 25~50 o C, enzymatic hydrolysis for 60~240 min; 6) After 60~80 min o C high temperature inactivates the enzyme to obtain the enzymatic rice protein with increased telomere length. The invention has low protease usage, high rice protein yield and high small molecular peptide content, and the obtained rice protein has the effect of increasing telomere length.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing enzymatically hydrolyzed rice protein with increased telomere length. Background technique [0002] Human normal cells will become senescent cells after dozens of natural passages, and each cell division will shorten the chromosomes, leading to the maturation and aging of cells. Inhibiting the aging of such cells requires the protection of telomeres in chromosomes. Telomere is a special structure at the end of eukaryotic chromosome, which is essentially a repetitive sequence, and its function is to maintain the integrity of chromosome. Telomerase is a ribonucleosome composed of protein and RNA. It is an RNA-dependent DNA polymerase that can add telomere fragments to the end of telomeres and repair the original length of telomeres. According to scientific research, injecting telomerase into aging cells can prolong the length of telomeres and make cells younger...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 晏青金激陈玉燕毕永贤席长洪蒋丽刚
Owner PROYA COSMETICS
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