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Dandelion seed antioxidant peptide and preparation method and application

A technology of dandelion and oxidized dipeptide, which is applied to the preparation method of peptides, chemical instruments and methods, food ingredients as antioxidants, etc., and can solve the problems of few studies on dandelion seeds

Inactive Publication Date: 2016-05-11
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on dandelion whole herb at home and abroad, and mainly on dandelion flavonoids, polysaccharides and terpenoids, but little research on dandelion seed proteins and peptides

Method used

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  • Dandelion seed antioxidant peptide and preparation method and application
  • Dandelion seed antioxidant peptide and preparation method and application
  • Dandelion seed antioxidant peptide and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of antioxidant peptide of the present invention is as follows:

[0021] Extraction of dandelion seed protein: dandelion seeds are pulverized by a high-speed traditional Chinese medicine grinder, extracted with 0.1mol / L NaOH solution, stirred at 40°C for 140min, centrifuged at 10000rpm for 20min, the supernatant was taken, and adjusted with 1mol / L hydrochloric acid. The pH value was 4.0, centrifuged at 12000 rpm for 20 min, and the precipitate was freeze-dried to obtain dandelion seed protein powder.

[0022] Enzymatic hydrolysis of dandelion seed protein: use alkaline protease to enzymolyze dandelion seed protein, the protein concentration is 2% (w / v), the enzyme-to-substrate ratio is 7.8%, the enzymatic hydrolysis time is 4.90h, pH8.5, temperature is 45℃, After the enzymatic hydrolysis was completed, the enzyme was inactivated in a boiling water bath for 10 minutes, then rapidly cooled to room temperature, centrifuged at 10,000 rpm for 20 minutes...

Embodiment 2

[0028] The natural antioxidant peptide activity that obtains in embodiment 1 is researched:

[0029] DPPH free radical scavenging activity: take 1mL of samples with different concentrations and 1mL of DPPH solution (0.1mM, prepared in 95% ethanol) and mix well, and let it stand at room temperature in the dark for 30min, and measure the absorbance at 517nm; use 1mL of 95% ethanol solution instead of DPPH solution as the sample The reference group; the blank group consisted of 1mL DPPH solution and 1mL95% ethanol solution. The DPPH free radical scavenging activity of the sample was calculated according to the following formula (1):

[0030] (1)

[0031] In the formula: A i —Absorbance value of sample group; A j — Absorbance value of sample reference group; A 0 — Absorbance value of the blank group

[0032] ABTS free radical scavenging activity: prepare 7mM ABTS storage stock solution and 2.45mM potassium persulfate solution, mix them at a ratio of 1:1 before use, place at...

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PUM

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Abstract

The invention discloses an amino acid sequence, a preparation method and application of antioxidant peptide from dandelion seeds. The sequence of the antioxidant peptide is Va1-Phe(VF), in-vitro experiments show that the peptide can effectively remove DPPH, ABTS, hydroxyl free radicals, superoxide anions and other free radicals. The involved antioxidant peptide has the advantages of being simple in structure, high in antioxidant activity and the like, can serve as a good substituent of existing artificially-synthesized antioxidant and has important value in the aspects of development and application of novel antioxidant food additives.

Description

technical field [0001] The invention relates to a dandelion seed antioxidant peptide and its preparation method and application, belonging to the field of food biotechnology. Background technique [0002] Antioxidants play a vital role in the oxidation process in food and in the human body. In foods, antioxidants can effectively delay the formation of lipid peroxides, reduce the oxidation of proteins and the interaction between lipid-derived carbonyls and proteins that lead to changes in protein functions, so that foods can be kept in their original state during storage. Unique texture, flavor, color and nutrition. In the human body, antioxidants protect tissues and organs from damage by reactive oxygen species. Synthetic antioxidants have a good function of scavenging free radicals, but synthetic antioxidants such as BHT and PG have certain toxic effects on the enzyme system of the human body, while natural antioxidants have both strong antioxidant activity and high safet...

Claims

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Application Information

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IPC IPC(8): C07K5/062C12P21/06C07K1/20C07K1/16A23L33/18
CPCA23V2002/00C07K5/06052A23V2200/02A23V2250/55
Inventor 洪晶张杨胡磊孟春
Owner FUZHOU UNIV
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