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Brevundimonas diminuta and its application

A Brevimonas, defect technology, applied in bacteria, biological water/sewage treatment, microorganisms, etc., can solve problems such as shortage, and achieve the effect of environmental friendliness and strong algae-dissolving effect

Active Publication Date: 2019-06-25
SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, in terms of algae-lytic bacteria, a variety of algae-lytic bacteria have been isolated from different environments. The screened algae-lytic bacteria involve many genera and species, including Bacillus, The species is mainly aimed at Microcystis aeruginosa, and there is a lack of bacterial agents that also have algicidal effects on other Microcystis species that cause water blooms.

Method used

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  • Brevundimonas diminuta and its application
  • Brevundimonas diminuta and its application
  • Brevundimonas diminuta and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Strain isolation

[0019] 1. Screening of algicidal bacteria

[0020] Take the natural soil as the source of bacteria, put the collected soil sample into a beaker, which accounts for about 2 / 3 of the volume of the beaker, add sterile water, stir evenly, let stand for 2 hours, and remove 10ml to 100ml of the supernatant. The sterilized beef extract peptone liquid medium was cultured at 37°C for 48h, and it was used for later use.

[0021] The beef extract peptone medium is composed of: peptone 5.0g, NaCl 5.0g, beef extract 5.0g, distilled water 1000ml, pH is about 7.5, for solid medium, need to add agar powder 2.0% (m / v) , the medium used should be sterilized at 120℃, 0.11Mpa for 30min.

[0022] Adjust the density of the bacterial liquid so that it is about 10 8 CFU / mL, according to the ratio of 1:10 (v / v), the bacterial solution was added to the Microcystis algal solution, and the algal cell density was about 10 9 Pieces / L, after 5-7 days, take the yellowe...

Embodiment 2

[0025] Example 2 Application of strain Brevundimonas diminuta in Microcystis algal lysis

[0026] The strain was inoculated into the sterilized beef extract peptone liquid medium, and cultured at 37°C for 48h, and the density of the bacteria was 1.0×10 9 -1.0×10 10 When CFU / mL, the bacterial liquid is added to the algal liquid according to the bacterial liquid: algal liquid volume percentage 1:10-5:10, and the algal cell density in the processed algal liquid is 10 11 pcs / L or less. It can be concluded that the strain Brevundimonasdiminuta has a high algae-dissolving ability to Microcystis, and the algae-dissolving efficiency can reach more than 98% (with chlorophyll a as the measurement index) after the bacteria and algae are mixed for 10 days. figure 1 , 2 shown).

Embodiment 3

[0028] The present embodiment is to treat Microcystis aeruginosa according to the bacterial solution: the algal solution volume ratio is 3:10. The specific implementation steps are as follows: inoculate Brevimonas deficient in 10ml of sterilized beef extract peptone liquid medium, cultivate at 37°C for 48 hours, and then transfer the bacterial liquid to 100ml of sterilized beef extract peptone liquid medium medium, incubate at 37°C for 48h, and adjust the density of the bacterial solution to about 10 9CFU / mL, and then add the bacterial solution to the algal solution according to the bacterial-algae volume ratio of 3:10. The initial concentration of chlorophyll a in the algal solution is 1531 μg / L. After adding the bacterial solution for 10 days, the chlorophyll concentration is 68.7μg / L, the algae-dissolving efficiency was 95.5%.

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Abstract

The invention belongs to the technical field of environmental protection, and specifically relates to Brevundimonas diminuta and its application, and its preservation number is: CGMCC No.11571. The bacterial strain screened by the invention has a strong algalytic effect on the dominant algal species of cyanobacteria bloom, Microcystis, and can completely remove Microcystis under certain conditions. The bacterial strain screened by the invention is friendly to the environment, does not produce secondary pollution, can be used in the production and application of a new type of algicide, and ultimately controls the bloom of the cyanobacteria Microcystis.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to Brevimonas deficient and applications thereof. Background technique [0002] With the rapid development of social economy and the continuous acceleration of urbanization, people have higher and higher requirements for the quality of life, and the demand for fresh water is also increasing. However, the pollutants in the discharge of production and domestic sewage have not been effectively reduced. The water environment has not been fundamentally improved. In particular, pollutants containing nitrogen and phosphorus are continuously discharged into the water body, which leads to the loss of the self-purification function of the water body. economic production and physical health. [0003] The outbreak of algal blooms reduces the dissolved oxygen in the water body, the water quality becomes smelly, the transparency of the water body decreases, and aqua...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 梁文艳樊乾龙张恒峰黄娟赵亮许建新王自力蔡飞徐军
Owner SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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