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A Strain of Stenotrophomonas and Its Application

A technology of Stenotrophomonas and culture medium, which is applied in the field of microorganisms, can solve the problems of secondary pollution of freshwater ecosystems, harm to effective use, high cost, etc., and achieve good application prospects, high repeatability, and simple screening methods Effect

Inactive Publication Date: 2015-10-14
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, physical and chemical methods limit their effective use due to high cost, secondary pollution, and potential harm to freshwater ecosystems

Method used

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  • A Strain of Stenotrophomonas and Its Application
  • A Strain of Stenotrophomonas and Its Application
  • A Strain of Stenotrophomonas and Its Application

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Embodiment Construction

[0028] The present invention is described in detail below with examples, but the protection scope of the present invention is not limited thereto.

[0029] 1. Screening of Alginolytic Bacteria

[0030] Inoculate 1mL of natural water sample collected from Meiliangwan waters of Taihu Lake into 100mL beef extract peptone medium for enrichment culture for 24h (fermentation culture at 30°C, 200rpm). Microcystis 9110 (isolated from Taihu Lake) algae liquid (algae cell density about 1×10 7 individual / mL). One week later, the yellow algae liquid was taken and spread on the beef extract peptone medium plate by gradient dilution method, cultivated overnight at 30°C, and a plate with moderate colony density was taken, and a series of strains were selected from the plate according to the difference in colony morphology.

[0031] The selected strains were inoculated into 100 mL of beef extract peptone medium at a ratio of 1%, and cultured at 30°C and 200 rpm for 24 hours, and then 10 mL ...

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Abstract

The invention provides a strain of stenotrophomonas sp., which is named as stenotrophomonas F6, and has a preservation number of CGMCC No.6547. Two active algicidal substances, namely cyclo(glycine-proline) and hydroquinone, are separated from the metabolism products of the stenotrophomonas F6, and then the substances are purified and identified. The half effective concentration of the cyclo(glycine-proline) on inhibiting microcystis aeruginosa 9110 is 9.5 mg / L, and the cyclo(glycine-proline) has no inhibiting effect on synechococcus BN60. The half effective concentrations of hydroquinone on inhibiting microcystis aeruginosa 9110 and synechococcus BN60 are 0.96 mg / L and 5.6 mg / L. The stenotrophomonas F6 and the active algicidal substances (cyclo(glycine-proline) and hydroquinone) can be applied to the development and production of novel algicide, and finally are applied to the control of cyanobacterial bloom in fresh water.

Description

technical field [0001] The invention relates to microorganisms, in particular to a strain of Stenotrophomonas and its application. Background technique [0002] The frequent occurrence of cyanobacterial blooms due to the increasing eutrophication of water bodies has caused serious environmental and economic problems, so it has attracted widespread attention. In China, many freshwater lakes (such as Taihu Lake, Dianchi Lake, Chaohu Lake, etc.) are seriously affected by cyanobacterial blooms. Therefore, it is very necessary to effectively control cyanobacterial blooms. [0003] At present, the methods for controlling cyanobacterial blooms mainly include physical methods, chemical methods and biological methods. Physical methods mainly include artificial fishing and mechanical algae removal. Chemical methods mainly include the addition of algicidal chemicals copper sulfate, herbicides, etc. However, physical and chemical methods limit their effective use due to high cost, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/50C12R1/01
Inventor 杨虹林升钦柳向龙谭晶
Owner SHANGHAI JIAO TONG UNIV
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