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Brevundimonas diminuta and application thereof

A brevemonas, defect technology, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve problems such as lack, and achieve the effect of environmental friendliness and strong algae-dissolving effect

Active Publication Date: 2016-05-11
SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, in terms of algae-lytic bacteria, a variety of algae-lytic bacteria have been isolated from different environments. The screened algae-lytic bacteria involve many genera and species, including Bacillus, The species is mainly aimed at Microcystis aeruginosa, and there is a lack of bacterial agents that also have algicidal effects on other Microcystis species that cause water blooms.

Method used

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  • Brevundimonas diminuta and application thereof
  • Brevundimonas diminuta and application thereof
  • Brevundimonas diminuta and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 strain isolation

[0019] 1. Screening of algae-lytic bacteria

[0020] Using the natural soil as the source of bacteria, put the collected soil sample into a beaker, accounting for about 2 / 3 of the volume of the beaker, add sterile water, stir evenly, let it stand for 2 hours, and pipette 10ml to 100ml of the supernatant In the sterilized beef extract peptone liquid medium, culture at 37°C for 48h, and set aside.

[0021] The beef extract peptone medium described therein consists of: peptone 5.0g, NaCl5.0g, beef extract 5.0g, distilled water 1000ml, pH is about 7.5, for solid medium, need to add agar powder 2.0% (m / v) , the medium used should be sterilized at 120°C, 0.11Mpa for 30min.

[0022] Adjust the density of the bacterial solution to about 10 8 CFU / mL, according to the ratio of 1:10 (v / v), the bacterial liquid was added to the Microcystis algae liquid, and the algal cell density was about 10 9 pc / L, after 5-7 days, take the yellowed algae liquid as...

Embodiment 2

[0025] Application of embodiment 2 bacterial strain Brevundimonas diminuta in Microcystis algae lysis

[0026] The strain was inoculated into the sterilized beef extract peptone liquid medium, and cultivated at 37°C for 48 hours, until the density of the bacteria was 1.0×10 9 -1.0×10 10 For CFU / mL, add the bacterial liquid to the algal liquid according to the volume percentage of bacterial liquid: algal liquid 1:10-5:10, and the algal cell density in the treated algal liquid is 10 11 A / L or less. It can be drawn that the bacterial strain Brevundimonas diminuta has a higher algicidal ability to Microcystis, and after the mixture of bacteria and algae, it is cultivated for 10 days, and the algalytic efficiency can reach more than 98% (taking chlorophyll a as a measurement index) (such as figure 1 , 2 shown).

Embodiment 3

[0028] In the present embodiment, Microcystis aeruginosa is treated at a bacterium injection volume ratio of 3:10 by bacteria liquid: algae liquid. The specific implementation steps are as follows: inoculate Brevundimonas diminuta in 10 ml of sterilized beef extract peptone liquid medium, culture at 37°C for 48 hours, and then transfer the bacterial solution to 100 ml of sterilized beef extract peptone liquid medium , cultured at 37°C for 48 hours, and adjusted the density of the bacterial solution to about 10 9CFU / mL, then add the bacterial solution to the algal liquid according to the bacterial volume ratio of 3:10, and measure the initial concentration of chlorophyll a in the algal liquid to be 1531 μg / L. After 10 days of adding the bacterial liquid, the chlorophyll concentration is 68.7μg / L, algae-dissolving efficiency is 95.5%.

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Abstract

The invention belongs to the technical field of environmental protection, and in particular, relates to brevundimonas diminuta and an application thereof, wherein the brevundimonas diminuta has the preservation number of CGMCC No.11571. The bacterial strain obtained through screening has strong algae dissolving effect on dominant-algae microcystis of cyanobacteria bloom, and can completely remove the microcystis under certain conditions. The bacterial strain obtained through screening is friendly to environment and cannot produce secondary pollution, can be used for production and application of novel algicides, and ultimately controls cyanobacteria microcystis bloom.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to Brevundimonas diminuta and its application. Background technique [0002] With the rapid development of social economy and the acceleration of urbanization, people have higher and higher requirements for the quality of life, and the demand for fresh water is also increasing. However, the pollutants in the discharge of production and domestic sewage have not been effectively reduced. The water environment has not been fundamentally improved. In particular, pollutants containing nitrogen and phosphorus are continuously discharged into the water body, resulting in the loss of the self-purification function of the water body, and the frequent outbreak of algae blooms in rivers and lakes, which seriously damages the ecological environment of the water body, threatens aquatic organisms, and seriously affects people's lives. Economic production and physical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 梁文艳樊乾龙张恒峰黄娟赵亮许建新王自力蔡飞徐军
Owner SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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