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Kit for detecting urea nitrogen in milk

A detection kit and technology of urea nitrogen, which is applied in the field of urea nitrogen detection, can solve the problems of cumbersome operation, high detection cost, and long time-consuming, and achieve the effect of convenient use and accurate detection

Inactive Publication Date: 2016-05-11
BEIJING VOCATIONAL COLLEGE OF AGRI
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Problems solved by technology

[0005] In order to solve the problems of cumbersome operation, high detection cost, long time-consuming, low sensitivity and specificity in the detection process of urea nitrogen in milk in the prior art, the present invention provides a detection kit for urea nitrogen in milk, The invention uses a 96-well reaction plate and a microplate reader for detection, saves reagents, has a large detection throughput, and also has milk treatment liquid for sample processing

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  • Kit for detecting urea nitrogen in milk
  • Kit for detecting urea nitrogen in milk
  • Kit for detecting urea nitrogen in milk

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Embodiment Construction

[0032] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0033] The invention provides a kit for detecting urea nitrogen in milk. The reagents in the kit include concentrated precipitant I, concentrated precipitant II, buffer solution, enzyme solution, chromogenic agent I, chromogenic agent II and control solution , also includes a 96-well plate with a cover, specifically:

[0034] 1. Preparation of treatment solution:

[0035] Concentrated Precipitant I: Weigh 4.24g of potassium ferrocyanide, dissolve it in ultrapure water and set the volume to 100mL.

[0036] Concentrated Precipitant II: Weigh 8.76g of zinc acetate, dissolve it in ultrapure water and make it up to 100mL.

[0037] 2. Buffer solution:

[0038] Weigh 1 g of sodium ethylenediamine tetraacetate and dissolve it in 100 mL of ultrapure water, and adjust the pH value to 6.5 with 1 mol / L sodium hydroxide solution.

[0039] 3. Enzyme solution: W...

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Abstract

The invention discloses a kit for detecting urea nitrogen in milk, and belongs to the technical field of detection of urea nitrogen in animal husbandry and animal nutrition and feed science. The kit comprises a 96-hole reacting plate, a first concentrating precipitant, a second concentrating precipitant, a buffering solution, an enzyme solution, a first color developing agent, a second color developing agent and a control solution. When the kit is applied, the difference between a sample-solution optical density value and a baseline optical density value is substituted into a urea-nitrogen absorption curve, and the content of urea nitrogen in a milk sample is computed. According to the kit, the 96-hole reacting plate and a microplate reader are adopted for detection, reagents are saved, the detecting flux is large, and meanwhile a milk treating solution exists to treat the sample. The kit is convenient to use and suitable for mass detection of the urea nitrogen in the milk.

Description

technical field [0001] The invention belongs to the technical field of urea nitrogen detection in animal husbandry, animal nutrition and feed science, and specifically refers to a kit for detecting urea nitrogen in milk. Background technique [0002] Utilizing milk urea nitrogen (MUN) to evaluate dietary energy nitrogen balance, protein requirement, nitrogen utilization rate, reproductive rate and diagnosis of metabolic diseases of dairy cows has become a hotspot in scientific research on dairy cows worldwide, and milk sampling is convenient and will not Stress the cows. [0003] The methods for determining MUN mainly include diacetyl-oxime method, urease-Bo's colorimetric method, enzyme-linked rate method, and infrared spectroscopy. Infrared spectroscopy is easy to operate and fast. The determination of DHI abroad includes the MUN index, which is rarely used in China. [0004] MUN detection kits are generally detected by spectrophotometry, and the amount of reagents used ...

Claims

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Application Information

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IPC IPC(8): C12Q1/58
CPCC12Q1/58
Inventor 马建民孙健乔利敏
Owner BEIJING VOCATIONAL COLLEGE OF AGRI