Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of miR-331-5p as acute HIV infection detection marker

An acute and material technology, applied in the field of acute HIV infection detection markers, can solve problems such as high price, and achieve the effects of convenient operation, high sensitivity and favorable clinical application.

Inactive Publication Date: 2016-05-11
BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In acute HIV infection, HIV antibodies have not yet been fully produced, and the diagnosis mainly relies on HIV antigen and nucleic acid detection. The sensitivity of HIV antigen detection is 30-90%, which is only the basis for auxiliary diagnosis of HIV infection, and HIV nucleic acid detection is expensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of miR-331-5p as acute HIV infection detection marker
  • Application of miR-331-5p as acute HIV infection detection marker
  • Application of miR-331-5p as acute HIV infection detection marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Practical verification of the correlation between miR-331-5p expression in clinical plasma samples and acute HIV infection

[0041] 1. Material and Specimen Collection Description

[0042] The samples were all from the dynamic follow-up cohort of high-risk gay men in You'an Hospital, followed up once every 2 months. After the whole blood was collected, the plasma and peripheral blood mononuclear cells were separated and frozen. The samples of 20 cases of acute HIV infection before and after infection were selected. , These infected persons were excluded from the merger of syphilis, hepatitis B and C.

[0043] 2. Plasma miRNA extraction

[0044] Plasma RNA was extracted and purified by Qiagen's miRNeasySerum / PlasmaKit kit according to the kit instructions. The specific steps are as follows:

[0045] 2.1 Thaw frozen plasma samples.

[0046] 2.2 Add 500 μL of QIAzolLysis Reagent to each 100 μL sample, and use a pipette to repeatedly blow and mix.

[0047] 2.3...

Embodiment 2

[0082] Embodiment 2 Preparation and use method of kit of the present invention

[0083] The composition of kit of the present invention:

[0084] 1. The primer sequence used to detect the expression level of miR-331-5p, the detection primer sequence is shown in 1 μL of the upstream primer SEQIDNo.3 (10 μM) and 1 μL of the downstream primer SEQIDNo.4 (10 μM).

[0085] 2. The PCR reaction solution can be a composition known to those skilled in the art. The recommended composition of the present invention is: 12.5 μL of PCR reaction solution, which contains SybergreenI fluorescent dye 0.5-1X, DNA polymerase 100U / mL, dNTPs0.2mM, trimethylol Aminomethane hydrochloride 16.5mM, potassium chloride 89.3mM, magnesium chloride 6mM and sterile water 5.5μL.

[0086] The method for using the kit of the present invention:

[0087] 1. Extraction of miRNA in the sample to be tested: Purchase Qiagen's miRNeasySerum / PlasmaKit kit and follow the kit instructions. Refer to the plasma miRNA extr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a substance for detecting the miR-331-5p expression level to preparation of a product for diagnosing or assisting in diagnosing a patient infected with acute HIV, and the nucleotide sequence of the miR-331-5p is as shown in SEQ ID NO.1. According to the application, a real-time quantification PCR technology is utilized, a sample to be tested is plasma of a person to be tested, the content of the miR-331-5p in the tested plasma is detected and compared with the miR-331-5p level of normal people, and acute HIV infection is diagnosed. The AUC of the acute HIV infection detection marker miR-331-5p in predicating acute HIV infection is 0.801 (the 95% confidence interval ranges from 0.65 to 0.95), both sensitivity and accuracy are 80%, sensitivity is high, specificity is high, operation is convenient to conduct, and clinical application is facilitated.

Description

technical field [0001] The present invention relates to a marker for detection of acute HIV infection, in particular to the application of miR-331-5p as a marker for detection of acute HIV infection. Background technique [0002] AIDS is a disease caused by human immunodeficiency virus (HIV) infection. After HIV infection, the virus phagocytizes and destroys T4 lymphocytes in large quantities, thereby destroying the entire human immune system, and eventually the human body loses its resistance to various diseases and dies. cause death. According to data released by the China Centers for Disease Control and Prevention, as of the end of October 2015, a total of 575,000 surviving HIV-infected and patients were reported nationwide, with 177,000 deaths. AIDS not only affects the life and health of the masses, but also seriously harms social stability and economic development. [0003] The diagnosis of acute HIV infection is conducive to early detection and treatment, and plays ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 张宏伟吴昊张彤张美李珍陆小凡夏炜张欣
Owner BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV