Animal sample grading fat removing method

A technology for samples and animals, which is applied in the field of grading and removing fat from animal samples, can solve the problems of the influence of the total amount, incomplete purification of fat, and high impurity content, and achieve the effect of reducing the impact, facilitating detection, and improving detection efficiency

Active Publication Date: 2016-05-11
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

The sample is not subjected to multi-stage purification treatment. After such a biological sample is purified, it is easy to cause high impurity content and incomplete fat purification, and the requirements for the total amount of biological samples will also be affected. If the sample volume is too large, then the purification is not complete

Method used

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  • Animal sample grading fat removing method
  • Animal sample grading fat removing method
  • Animal sample grading fat removing method

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Embodiment Construction

[0023] Taking PBDE purification as an example, according to figure 1 In the shown process, the fat in the fish muscle sample is processed by adopting the method for fractionating and removing fat of the animal sample provided by the present invention.

[0024] (1) Extraction and primary purification of fish muscle samples

[0025] Add 2g of freeze-dried dried fish powder to the indicator of recovery rate 13 C-labeled PBDE mix, 13 C-labeled BDE-3, 15, 28, 47, 99, 153, 154, 183, 197, 207, 209. A mixture of dichloromethane and acetone with a V / V ratio of 1:1 was used as the solvent, and the solvent was 100 mL for Soxhlet extraction for 24 h.

[0026] After the sample extraction is completed, the extract after the sample extraction is concentrated to near dryness by rotation, and the sample after the rotary evaporation is transferred to a 22mL sample bottle with 8mL of n-hexane, and 6mL of concentrated sulfuric acid is added, vortexed, mixed evenly, and then centrifuged. The c...

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Abstract

The invention relates to a biological sample treatment method, in particular to an animal sample grading fat removing method, comprising the steps of (1) concentrated sulfuric acid defatting and sample primary cleaning; (2) solvent leaching and collecting, and sample secondary cleaning. The animal sample grading fat removing method can more thoroughly remove fat substances in an animal sample, effectively reduces influence of the fat substances on subsequent instruments, improves sample detection efficiency, reduces a sample detection limit and is more favorable for detection of trace nonpolar organic pollutant.

Description

technical field [0001] The invention relates to a biological sample processing method, in particular to a method for grading and removing fat from animal samples. Background technique [0002] Due to the high fat content of biological samples, the presence of fat has a greater impact on the instrument during sample analysis, resulting in an increase in the baseline of the instrument and affecting the accuracy of the instrument's determination. Therefore, the fat in the sample must be removed before sample analysis to reduce damage to the instrument. [0003] In the existing technologies, most of them adopt primary purification treatment, only using chromatographic column, and the chromatographic column is a column. The sample is not subjected to multi-stage purification treatment. After such a biological sample is purified, it is easy to cause high impurity content and incomplete fat purification, and the requirements for the total amount of biological samples will also be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/8813
Inventor 冯承莲吴丰昌符志友李小峰张旭
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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