Antibacterial hairtail tetrapeptide and preparation method thereof

Abstract

The invention discloses an antibacterial hairtail tetrapeptide with an amino acid sequence of Gln-Ala-Glu-Gly. The invention provides a novel antioxidative peptide, i.e., hairtail tetrapeptide which is prepared through hydrolysis of alkali protease and has a hydrolysis degree of 69.52% and a hydroxyl radical scavenging rate of 76.74%. The hairtail tetrapeptide is also a novel antibacterial peptide and has strong antibacterial activity; and a hairtail tetrapeptide filtrate obtained in the invention has antibacterial activity of 85.2% and antioxidative activity of 77.8%. As the hairtail tetrapeptide is applied to Staphylococcus aureus, a minimal inhibitory concentration (MIC) is 0.20 mg / ml, and a minimal bactericidal concentration (MBC) is 0.26 mg / ml. A preparation method for the hairtail tetrapeptide in the invention is simple and only needs one-step hydrolysis; hydrolysis conditions are mild; purifying process is simple; the preparation method is applicable to any scale-up industrial production; and alkali protease is employed for hydrolysis and a hydrolysis degree is 69.52%.

Description

technical field [0001] The invention relates to an antibacterial peptide isolated from hairtail protein, belonging to the field of amino acid extraction technology. Background technique [0002] Antimicrobial peptides are small molecular polypeptides in organisms and are important components of the innate immune system of organisms. Antimicrobial peptides have broad-spectrum antibacterial and antiviral properties, and it is more difficult for bacteria to develop resistance to antimicrobial peptides than traditional antibiotics, so they are ideal substitutes for traditional antibiotics. More than 2,000 antimicrobial peptides have been isolated from various organisms. There are many kinds of fish and their habitats are complex, and they are an important source of antimicrobial peptides. So far, more than 150 fish-derived antimicrobial peptides have been reported. Liu Chao (Based on enzymatic preparation of catfish antimicrobial peptide purification test, Food Industry, 2014)...

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Problems solved by technology

Fish Our country is a big country of aquatic products, and the output of hairtail is high. However, so far, there has not been extensive research on the production of antimicrobial peptides by enzymatic hydrolysis of aquatic products. There has been no relevant literature on the separation and extraction of protein from hairtail with high quality protein content.

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