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Preparation method of blood synchronous separation and sensing film

A technology of synchronous separation and sensing membrane, which is applied in the preparation of test samples, analysis materials, sampling, etc., to achieve the effect of simple process, low cost and good acid and alkali resistance

Active Publication Date: 2016-06-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above two technologies are quite different in material selection and working mechanism, and there is no research result that can integrate the two and manufacture corresponding products

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  • Preparation method of blood synchronous separation and sensing film

Examples

Experimental program
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Effect test

Embodiment 1

[0017] 1) Formulated with K 4 Fe(CN) 6 Solution of KCl aqueous solution, prepared with FeCl 3 The KCl aqueous solution of the solution has an anion and cation concentration of 0.001M, a KCl concentration of 0.01M, and a pH of 1.

[0018] 2) Seal one end of the support body, connect the other end to a vacuum pump, control the ambient temperature at 0°C, immerse the support body in the K ion aqueous solution containing anion solution for 10 minutes and take it out; then immerse the support body in deionized water for 10 seconds and take it out; then Immerse the support body in the KCl aqueous solution containing the cation solution for 10 minutes and take it out; finally immerse the support body in deionized water for 10 seconds and take it out. The above operation was repeated 60 times, and the film thickness reached 1 μm. Dry the prepared film. The scanning electron microscope image of the cross-section of the prepared film is shown in figure 1 shown.

Embodiment 2

[0020] 1) Formulated with K 3 Fe(CN) 6 K of the solution 2 SO 4 Aqueous solution, prepared containing (NH 4 ) 2 Fe(SO 4 ) 2 K of the solution 2 SO 4 Aqueous solution, the concentration of anions and cations is 0.1M, K 2 SO 4 The concentration is 0.5M and the pH is 9.

[0021] 2) Seal one end of the support body, and connect the other end to a vacuum pump. The ambient temperature is controlled at 60°C, and the support body is immersed in the KCl aqueous solution containing anion solution for 1 min and then taken out; then the support body is immersed in deionized water for 60 seconds and taken out; then Immerse the support body in the KCl aqueous solution containing the cation solution for 1 min and take it out; finally immerse the support body in deionized water for 60 seconds and take it out. The above operation was repeated once, and the film thickness reached 2 μm. The prepared membrane was dried naturally.

Embodiment 3

[0023] 1) Formulated with K 3 Co(CN) 6 KNO of solution 3 Aqueous solution, prepared with CoCl 3 KNO of solution 3 Aqueous solution, the concentration of anion and cation is 0.01M, the concentration of K ion is 0.05M, and the pH is 3.

[0024] 1) Seal one end of the support body, connect the other end to a vacuum pump, control the ambient temperature at 35°C, immerse the support body in the K ion aqueous solution containing anion solution for 5 minutes and take it out; then immerse the support body in deionized water for 30 seconds and take it out; Then, the support body was immersed in the K ion aqueous solution containing the cation solution for 5 minutes, and then taken out; finally, the support body was immersed in deionized water for 30 seconds, and then taken out. The above operation was repeated 10 times, and the film thickness reached 2 μm. Dry the prepared film.

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Abstract

The invention relates to a preparation method of a blood synchronous separation and sensing film. The preparation method comprises the following concrete steps of respectively preparing a K ion water solution containing an anion solution and a K ion water solution containing a cation solution, immersing a support body into a KCL water solution containing the anion solution, and taking out the support body; then, immersing the support body into deionized water, and taking out the support body; next, merging the support body into the KCL water solution containing the anion solution, and then taking out the support body; finally, merging the support body into the deionized water, and then taking out the support body to complete the deposition of a layer of film. Repeated steeping is performed for controlling the film to reach a certain thickness; drying is performed, and a film prepared by the blood synchronous separation and sensing film is obtained; the prepared film can be applied to fast and real-time extraction of fresh blood; meanwhile, the concentration detection of important ingredients of blood glucose, blood lactic acid, blood glutamic acid, aminotransferase, protein and the like is performed. The preparation method has the advantages that the process is simple; the cost is low; good large-scale production prospects are realized.

Description

technical field [0001] The present invention relates to a method for preparing blood synchronously separating and sensing membrane, in particular to a method for preparing synchronously separating serum and component concentration sensing membrane in blood, and the prepared membrane can be used in the detection of serum in fresh blood Rapid real-time extraction and simultaneous detection of the concentration of important components such as blood sugar, blood lactic acid, blood glutamate, transaminase, and protein. Background technique [0002] In modern medicine, the extraction and detection of serum has become one of the most important means in the process of disease diagnosis. In the methods currently used in hospitals, both extraction and detection require independent operations and special instruments: serum extraction mainly relies on coagulation and centrifugation to separate blood cells, fibrinogen and serum; serum detection mainly relies on commercialized Biochemica...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N1/40
CPCG01N1/4005G01N27/26G01N2001/4016
Inventor 金万勤储震宇彭京蒙
Owner NANJING UNIV OF TECH