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Application of FAN1 genes in II-type diabetes diagnosis

A diabetes and gene technology, applied in the application field of FAN1 gene in the diagnosis of type II diabetes, to achieve the effect of timely diagnosis and early diagnosis

Active Publication Date: 2016-06-08
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the diagnosis of type II diabetes mainly relies on laboratory tests such as urine sugar, urine ketone, urine albumin, blood electrolytes, blood urea nitrogen and creatinine and various auxiliary tests, but there is no reliable clinical biomarker for type II diabetes. Risk prediction and early diagnosis of diabetes

Method used

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  • Application of FAN1 genes in II-type diabetes diagnosis
  • Application of FAN1 genes in II-type diabetes diagnosis
  • Application of FAN1 genes in II-type diabetes diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening for Gene Markers Related to Type II Diabetes Mellitus

[0048] 1. Sample collection

[0049] 10 normal blood samples and blood samples from patients with type 2 diabetes were collected, and all the above samples were obtained with the consent of the ethics committee.

[0050] 2. RNA sample preparation and quality analysis

[0051] 2.1 Preparation of RNA samples

[0052] (1) Homogenization treatment

[0053] Take blood directly, add 3 times the volume of erythrocyte lysate, mix well, place at room temperature for 10 minutes, and centrifuge at 10,000 rpm for 1 minute. Discard the supernatant thoroughly and collect the white blood cell pellet. Add 1ml Trizol per 100-200μl blood collected leukocyte pellet.

[0054] (2) layered

[0055] a. After adding TRizol to the sample, place it at room temperature for 5 minutes to fully lyse the sample. Centrifuge at 12,000rpm at 4°C for 10min, and take the supernatant;

[0056] b. Add 200 μl of chloroform to...

Embodiment 2

[0078] Example 2 QPCR sequencing to verify the differential expression of the FAN1 gene

[0079] 1. According to the detection results of high-throughput sequencing, the FAN1 gene was selected for large-sample QPCR verification. According to the sample collection method in Example 1, 70 cases of blood from patients with type II diabetes and 70 cases of normal blood were selected.

[0080] 2. The RNA extraction steps are the same as in Example 1.

[0081] 3. Reverse transcription: use the reverse transcription kit of TAKARA company to operate. Specific steps are as follows:

[0082] (1) Take 2 μg of total RNA for reverse transcription, add 2 μl of OlIIgo(dT), and mix well; 70°C water bath; immediately after 5 min, ice bath for 2-3 min;

[0083] (2) Construct a 25 μl reaction system, including 5 μl of 5× reverse transcription buffer, 5 μl of dNTP (2.5 mM), 40 U / μl of RNasIIn, 200 U / μl of M-MLV, and make up to 25 μl of nuclease-free water;

[0084] (3) After 60 min in water b...

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Abstract

The invention discloses an application of FAN1 genes in II-type diabetes diagnosis. The expression level of the FAN1 genes in blood of a patient suffering from II-type diabetes is higher than that of the FAN1 genes in blood of healthy people, whether a patient suffers from diabetes can be diagnosed by detecting the expression level of the FAN1 genes in the blood of the patient, and accordingly, early treatment of the patients can be realized.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of the FAN1 gene in the diagnosis of type II diabetes. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. The long-term high blood sugar in diabetes leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. With the continuous improvement of people's living standards around the world, the number of diabetic patients is also increasing rapidly. The World Health Organization (WHO) estimates that by 2030, the number of people with diabetes worldwide will exceed 350 million. Due to the increasing incidence of diabetes, its incurable chronic nature, and the serious health risks associated with its complications (including but not limited to cardiovascular dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2800/042
Inventor 宋宏涛肖枫
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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