Molecular marker affecting daily gain character of pigs and application of molecular marker

A molecular marker and trait technology, applied in the field of molecular biotechnology and molecular markers, can solve the problems of time-consuming, labor-intensive, and high measurement costs, and achieve the effect of improving economic benefits and increasing the daily weight gain breeding process.

Active Publication Date: 2016-06-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional method of pig breeding is to evaluate the slaughter of the siblings or descendants of the target individual,...

Method used

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  • Molecular marker affecting daily gain character of pigs and application of molecular marker
  • Molecular marker affecting daily gain character of pigs and application of molecular marker
  • Molecular marker affecting daily gain character of pigs and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 is to specifically explain the inventive process of obtaining the daily weight gain performance of the present invention.

[0023] The body weight age of pigs at 30kg was measured by OSBORNE system and recorded as Day1, and the body weight age of pigs at 100Kg was recorded as Day2. The formula for calculating daily weight gain is: 70 / (Day2-Day1). The above experiment was carried out in the Shahu Breeding Pig Farm of Guangdong Wenshi Food Group Co., Ltd. During the measurement period, 10-12 pigs (each pig occupying an area of ​​2 square meters) were raised in each pen for free access to food and water, and were raised in a unified manner. Standard, uniform feeding ration.

Embodiment 2

[0024] Example 2 is to specifically explain the inventive process of obtaining the gene marker in the present invention.

[0025] (1) The DNA extraction method of Duroc pig ear-like tissue refers to the marked phenol-chloroform method to extract the whole genome DNA. The DNA quality and concentration of the pure Duroc population were tested with a Nanodrop-ND1000 spectrophotometer. The ratio of A260 / 280 is 1.8-2.0, and the ratio of A260 / 230 is 1.7-1.9. Finally, the qualified DNA samples were uniformly diluted to 50 ng / μl.

[0026] (2) Detection of 60KSNP genotype in the whole pig genome: use the Illumina Infinium SNP typing platform to order the ProcineSNP60DNAAnalysisKit chip. Then perform chip hybridization and result scanning according to the instructions and standard procedures of Illumina Infinium. Finally, the genotype data were read by GenomeStudio software. The quality control of the obtained genotype data was carried out with PLINKv1.07, and the detection rate of ...

Embodiment 3

[0028] Embodiment 3 specifically explains the inventive process of the invention for detecting SNP markers.

[0029] (1) Amplification of the target fragment containing the SNP site significantly related to Duroc daily weight gain performance The target fragment is a 1321bp nucleotide sequence in chromosome 1, and the upstream and downstream primers for sequence amplification are:

[0030] Upstream primer 5'-TCTTGCTGAATCCGCACTCGC-3';

[0031] Downstream primer 5'-CAGCACAGAAGAAAGCGAAGC-3'.

[0032] (2) PCR amplification system and condition setting

[0033] Configure 10ul system, including DNA sample 1ul, upstream primer 0.2ul, downstream primer 0.2ul, PCRmix 5ul, ddH 2 O3.6ul, PCR conditions were 94°C pre-denaturation for 5min, 94°C denaturation for 30s, 65°C annealing for 30s, 72°C extension for 80s, a total of 35 cycles, and the final extension was 72°C for 10min.

[0034] (3) DNA sequence sequencing identification: Sequence sequencing was carried out in Shenzhen Huada Ge...

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Abstract

The invention discloses a molecular marker affecting the daily gain character of pigs and an application of the molecular marker and belongs to the technical field of molecular biological technologies and molecular marker technologies. The site of the molecular marker is shown in SEQ ID NO:1 and located at the 466th nucleic acid single-base mutation (named g466G>T) of a sequence fragment. SNP (single nucleotide polymorphism) mutates at G>T at the 306869183th (306869183bpth) nucleotide site on a No.1 chromosome of the Version 10.2 of the international pig reference genome. The invention further provides the application of the SNP marker to genetic improvement of the Duroc daily gain character. A predominant allele of the SNP is preferred, the genetic progress of the Duroc daily gain character can be increased, and the breeding time of the Duroc daily gain character can be shortened, so that the economic benefit of breeding of boars is effectively increased.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and in particular relates to a molecular marker affecting daily weight gain traits of pigs and its application. Background technique [0002] Daily gain is an important index to evaluate the growth performance of pigs, so it is the focus of research on genetic improvement of breeding pigs. The traditional method of pig breeding is to evaluate by slaughtering the siblings or descendants of the target individual. The accuracy is high and the effect is remarkable, but it has the disadvantages of high cost, time-consuming and labor-intensive testing. Genetic improvement methods based on molecular markers of major genes are economical, fast and effective, and are a necessary supplement to traditional pig breeding (Zhou Lisheng et al., 2014). [0003] Duroc breeding pigs are widely used as the terminal male parent of commercial pigs because of their fast weight g...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴珍芳杨杰全建平蔡更元刘德武郑恩琴顾婷
Owner SOUTH CHINA AGRI UNIV
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