Method for separating and extracting tetrahydropyrimidine from fermentation broth

A technology of tetrahydropyrimidine and fermentation broth, applied in organic chemistry and other directions, can solve the problems of complex process route, high energy consumption and water consumption, difficult separation and purification, etc., and achieves high retention rate, low energy consumption, and improved recovery rate and purity. Effect

Active Publication Date: 2016-06-15
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a novel separation and extraction process for ectoine, which overcomes the problems of complex process routes, difficult separation and purification, high energy consumption and water consumption in the existing production process.

Method used

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  • Method for separating and extracting tetrahydropyrimidine from fermentation broth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] a, get ectoine fermented liquid 5L (ectoline content 25.0g / L), regulate pH with the hydrochloric acid of 4mol / L and be 4.0, enter microfiltration membrane separation system, obtain the ectoine microfiltrate that removes thalline; The microfiltration membrane separation system uses a hollow fiber microfiltration membrane with a pore size of 0.02 μm, an operating temperature of 30° C., and an operating pressure of 0.2 MPa. Collect the filtrate, stop collecting when the concentration of ectoine in the filtrate is lower than 1.0g / L, and the cell removal rate is 99.2%.

[0029] b. Pump the microfiltrate obtained in step a into the ultrafiltration membrane separation system to obtain the ectoine ultrafiltrate from which protein and pigment have been removed. The ultrafiltration membrane separation system adopts a hollow fiber ultrafiltration membrane with a pore size of 0.003 μm, an operating temperature of 40° C., and an operating pressure of 0.3 MPa. The filtrate is collect...

Embodiment 2

[0036]a, get ectoine fermented liquid 5L (ectoline content 25.0g / L), regulate pH with the hydrochloric acid of 4mol / L and be 6.0, enter microfiltration membrane separation system, obtain the ectoine microfiltrate that removes thalline; The microfiltration membrane separation system uses a hollow fiber microfiltration membrane with a pore size of 0.05 μm, an operating temperature of 35° C., and an operating pressure of 0.15 MPa. Collect the filtrate, stop collecting when the concentration of ectoine in the filtrate is lower than 1.0g / L, and the bacterial cell removal rate is 97.4%.

[0037] b. Pump the microfiltrate obtained in step a into the ultrafiltration membrane separation system to obtain the ectoine ultrafiltrate from which protein and pigment have been removed. The ultrafiltration membrane separation system adopts a hollow fiber ultrafiltration membrane with a pore size of 0.01 μm, an operating temperature of 35° C., and an operating pressure of 0.1 MPa. The filtrate i...

Embodiment 3

[0044] a, get ectoine fermented liquid 5L (ectoline content 25.0g / L), regulate pH with 4mol / L hydrochloric acid and be 5.0, enter microfiltration membrane separation system, obtain the ectoine microfiltrate that removes thalline; The microfiltration membrane separation system described above adopts a hollow fiber microfiltration membrane with a pore size of 0.12 μm, an operating temperature of 45° C., and an operating pressure of 0.1 MPa. Collect the filtrate, stop collecting when the concentration of ectoine in the filtrate is lower than 1.0g / L, and the bacterium removal rate is 94.6%.

[0045] b. Pump the microfiltrate obtained in step a into the ultrafiltration membrane separation system to obtain the ectoine ultrafiltrate from which protein and pigment have been removed. The ultrafiltration membrane separation system adopts a hollow fiber ultrafiltration membrane with a pore size of 0.006 μm, an operating temperature of 25° C., and an operating pressure of 0.2 MPa. The fil...

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Abstract

The invention provides a method for separating and extracting tetrahydropyrimidine from fermentation broth. The method solves the problem that the existing production technology has a complex technical route, high separating purification difficulty and high energy and water consumption. The method for separating and extracting tetrahydropyrimidine from tetrahydropyrimidine-rich fermentation broth comprises filtering to remove bacteria, most of proteins and a part of a pigment through a double-membrane separation system comprising a microfiltration membrane and an ultrafilter membrane, adsorbing tetrahydropyrimidine through a cation exchange resin, carrying out elution on tetrahydropyrimidine through ammoniacal liquor and carrying out active carbon decoloration, concentration alcohol precipitation, recrystallization and finished product drying to obtain tetrahydropyrimidine crystals.

Description

technical field [0001] The invention relates to a method for separating and extracting ectoine from fermentation broth, and belongs to the technical field of amino acid derivatives produced by fermentation. Background technique [0002] Ectoine, also known as 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid, English scientific name is 1,4,5,6-tetrahydro-2-methyl-4 -pyrimidinecarboxylic acid. It is a cyclic amino acid derivative with a relative molecular weight of 142.16 and a molecular formula of C 6 h 10 N 2 o 2 , an amino group and a carboxyl group in its molecular structure make it have the amphoteric properties of amino acids. Tetrahydropyrimidine is the energy substance of microbial cells, the osmotic pressure regulating substance and the biological protection agent of cells and macromolecular substances, which can alleviate the effects of hypertonicity, high temperature, freeze-thawing, drying, radiation and chemical reagents on proteins, nucleic acids, bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D239/06
CPCC07D239/06
Inventor 陈宁范晓光宁义科徐庆阳谢希贤张成林李燕军
Owner TIANJIN UNIV OF SCI & TECH
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