Guava leaf effective part with anti-tumor effect and preparation method and application thereof

Abstract

The invention discloses a guava leaf effective part with the anti-tumor effect and a preparation method and application thereof.The preparation method comprises the following steps that guava leaves are extracted through an alcoholic solution, filtered and subjected to vacuum concentration to obtain a total extract; water is added into the total extract for dispersion, petroleum ether and chloroform are sequentially used for extraction, and chloroform extraction parts are collected; the chloroform extraction parts are subjected to silica-gel column chromatography and sequentially eluted through the chloroform and methyl alcohol, wherein the ratio of the chloroform to the methyl alcohol is 0.9-1.1:0.9-1.1; the eluted part with the ratio of the chloroform to the methyl alcohol being 0.9-1.1:0.9-1.1 is collected, and the guava leaf effective part with the anti-tumor effect is obtained and can be applied in anti-tumor medicine.The effective part, having anti-tumor activity, in the guava leaf extract and main ingredients contained in the effective part can be determined, and a foundation is provided for follow-up quality control, medicine preparation and access to international markets.

Description

technical field [0001] The invention relates to an effective part of guava leaves with an anti-tumor effect, a preparation method and application thereof, and belongs to the technical field of anti-tumor drugs. Background technique [0002] Guava (PsidiumguajavaL.), commonly known as plucked fruit, chicken fruit, guava, red heart fruit, trumpet fruit, etc., is a plant of the genus Guava in the Myrtle family. It is an evergreen small tree or shrub. Widely distributed and planted in tropical and subtropical regions. [0003] In the prior art, guava leaf extract has the effect on adipose tissue tumor necrosis factor (TNF-α), peroxisome proliferator-activated receptor-γ (PPAR-γ) gene by regulating the mechanism of liver glucose metabolism AKT signaling pathway. It was found that the volatile oil components of guava leaves had the highest anti-proliferation activity on epidermal cancer cells, which was 4.37 times that of vinblastine. Another study believed that the anti-tumor ce...

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Problems solved by technology

[0004] However, in the prior art, although it has been reported that the guava leaf extract can play an anti-tumor effect, the existing research has not fully clarified which main components play an anti-tumor effect in the guava leaf extract. Even if it is proposed in the prior art that a certain monomer extracted from guava leaves has an anti-tumor effect, because it is a monomer component, it is impossible to indicate the specific effective part, and the synergy between the components of the effective part also leads to the inability to treat the tumor. The effective part of the guava extract is subject to effective quality control, so that the effective part of the extract can be used as a preparation; what's more, due to the unclear ingredients, the guava extract cannot be made into medicine and cannot enter the international market

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