Guava leaf effective part with anti-tumor effect and preparation method and application thereof

An anti-tumor effect, the technology of guava leaves, applied in the field of anti-tumor drugs, can solve the problems of unclear ingredients, inability to enter the international market, inability to control the quality of effective parts of guava extracts, etc.

Active Publication Date: 2016-06-22
深圳市华科健康投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the prior art, although it has been reported that the guava leaf extract can play an anti-tumor effect, the existing research has not fully clarified which main components play an anti-tumor effect in the guava leaf extract. Even if it is proposed in the prior art that a certain monomer extracted from guava leaves has an anti-tumor effect, because it is a monomer component, it is impossible to indicate

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  • Guava leaf effective part with anti-tumor effect and preparation method and application thereof
  • Guava leaf effective part with anti-tumor effect and preparation method and application thereof
  • Guava leaf effective part with anti-tumor effect and preparation method and application thereof

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preparation example Construction

[0034] The present invention is a kind of preparation method of guava leaf effective part with antitumor effect, comprises the following steps:

[0035] (1) Extract guava leaves with alcohol solution, filter, and concentrate under reduced pressure to obtain the total extract. Concentration under reduced pressure is carried out according to the conventional method, until the total extract has no alcohol smell; generally, it is concentrated to 1 / 10 of the weight of the original extract, which is easy to disperse with water in the following conditions. The alcohol solution is preferably ethanol, ethanol aqueous solution, methanol or methanol aqueous solution; in the ethanol aqueous solution, the volume percent concentration of ethanol is preferably greater than or equal to 70%; in the methanol aqueous solution, the volume percent concentration of methanol is preferably greater than or equal to 70%. The extraction method is preferably percolation, ultrasonic or heating to reflux. ...

Embodiment 1

[0041] The present embodiment provides a kind of preparation method of the guava leaf effective part with antitumor effect, comprises the following steps:

[0042] The guava leaves were extracted 5 times under reflux with 10 times the weight of alcohol solution (78wt% ethanol solution), filtered, and concentrated under reduced pressure to obtain the total extract;

[0043] Add water to the total extract and disperse at 1:1, extract with 2 times the volume of petroleum ether and chloroform successively, extract 5 times each, and collect the chloroform extraction parts;

[0044] Carry out silica gel column chromatography to the chloroform extraction part, 3 times of silica gel mixing samples are put on the column, successively eluted with 5 times of column volumes of chloroform, 5 times of column volumes of chloroform:methanol=1:1 elution, 5 times of column volumes of methanol Elution;

[0045] Collect chloroform:methanol=1:1 elution fraction, obtain the guava leaf effective fr...

Embodiment 2

[0095] The present embodiment provides a kind of preparation method of the guava leaf effective part with antitumor effect, comprises the following steps:

[0096] The guava leaves were ultrasonically extracted 3 times with 5 times the weight of alcohol solution (90wt% ethanol solution), filtered, and concentrated under reduced pressure to obtain the total extract;

[0097] Add water to the total extract and disperse at 1:1, sequentially extract with 1 volume of petroleum ether and chloroform, extract 7 times each, and collect the chloroform extraction parts;

[0098] Carry out silica gel column chromatography to the chloroform extraction part, 3 times of silica gel mixing samples are put on the column, successively eluted with 5 times of column volumes of chloroform, 5 times of column volumes of chloroform:methanol=1:1 elution, 5 times of column volumes of methanol Elution;

[0099] Collect chloroform:methanol=1:1 elution fraction, obtain the guava leaf effective fraction wi...

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Abstract

The invention discloses a guava leaf effective part with the anti-tumor effect and a preparation method and application thereof.The preparation method comprises the following steps that guava leaves are extracted through an alcoholic solution, filtered and subjected to vacuum concentration to obtain a total extract; water is added into the total extract for dispersion, petroleum ether and chloroform are sequentially used for extraction, and chloroform extraction parts are collected; the chloroform extraction parts are subjected to silica-gel column chromatography and sequentially eluted through the chloroform and methyl alcohol, wherein the ratio of the chloroform to the methyl alcohol is 0.9-1.1:0.9-1.1; the eluted part with the ratio of the chloroform to the methyl alcohol being 0.9-1.1:0.9-1.1 is collected, and the guava leaf effective part with the anti-tumor effect is obtained and can be applied in anti-tumor medicine.The effective part, having anti-tumor activity, in the guava leaf extract and main ingredients contained in the effective part can be determined, and a foundation is provided for follow-up quality control, medicine preparation and access to international markets.

Description

technical field [0001] The invention relates to an effective part of guava leaves with an anti-tumor effect, a preparation method and application thereof, and belongs to the technical field of anti-tumor drugs. Background technique [0002] Guava (PsidiumguajavaL.), commonly known as plucked fruit, chicken fruit, guava, red heart fruit, trumpet fruit, etc., is a plant of the genus Guava in the Myrtle family. It is an evergreen small tree or shrub. Widely distributed and planted in tropical and subtropical regions. [0003] In the prior art, guava leaf extract has the effect on adipose tissue tumor necrosis factor (TNF-α), peroxisome proliferator-activated receptor-γ (PPAR-γ) gene by regulating the mechanism of liver glucose metabolism AKT signaling pathway. It was found that the volatile oil components of guava leaves had the highest anti-proliferation activity on epidermal cancer cells, which was 4.37 times that of vinblastine. Another study believed that the anti-tumor ce...

Claims

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Application Information

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IPC IPC(8): A61K31/7024A61K31/56A61P35/00
CPCA61K31/56A61K31/7024A61K2300/00
Inventor 邵萌范钦刘远亮朱道琦汤庆发刘俊珊
Owner 深圳市华科健康投资有限公司
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