Preparation method of clonazepam molecularly imprinted stirring rod coating

A technology of molecular imprinting and clonazepam, which is applied in the field of analysis and detection, can solve the problems of time-consuming and cumbersome coating preparation, and achieve the effects of improving the effective utilization rate, enhancing the recognition and adsorption capacity, and optimizing the test conditions

Inactive Publication Date: 2018-10-26
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clonazepam molecularly imprinted stirring rod coating is used to separate and enrich benzodiazepines in complex samples, which overcomes the shortcomings of molecularly imprinted stirring rods, which are limited in the application of polar solvents, and the preparation of the coating is cumbersome and time-consuming.

Method used

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  • Preparation method of clonazepam molecularly imprinted stirring rod coating
  • Preparation method of clonazepam molecularly imprinted stirring rod coating
  • Preparation method of clonazepam molecularly imprinted stirring rod coating

Examples

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Effect test

Embodiment 1

[0051] (1) Preparation and activation of a magnetic stirring rod: Put a thin magnetic iron rod into a glass tube, sinter and seal both ends to make a magnetic stirring rod; Wash with water until neutral and activate; place the activated stirring bar in a silane coupling agent for silanization reaction, wash with methanol, and blow dry with nitrogen; the alkali solution is 1mol / L NaOH solution; the acid solution is 0.5mol / L L of hydrochloric acid solution; the soaking time is 18h and 2h respectively, and the silane coupling agent is a γ-methacryloxypropyltrimethoxysilane acetone solution with a mass concentration of 20%; the diameter of the magnetic stirring rod is 0.3cm, and the length It is 1.1cm. The silanization time is 1h.

[0052] (2) Magnetic nanoparticles modified with terminal diaminobenzyl carboxyl groups (gf-Fe 3 o 4 ) preparation: 1g Fe 3 o 4 @SiO 2 Disperse nanoparticles in toluene, add 20mL silane coupling agent, react at 110°C for 25h under nitrogen protect...

Embodiment 2

[0056] (1) Preparation and activation of a magnetic stirring rod: Put a thin magnetic iron rod into a glass tube, sinter and seal both ends to make a magnetic stirring rod; Wash with water until neutral and activate; place the activated stirring bar in a silane coupling agent for silanization reaction, wash with methanol, and blow dry with nitrogen; the alkali solution is 1mol / L NaOH solution; the acid solution is 0.5mol / L L of hydrochloric acid solution; the soaking time is 18h and 2h respectively, and the silane coupling agent that reacts with the stirring bar is a γ-methacryloxypropyltrimethoxysilane acetone solution with a mass concentration of 20%; the diameter of the magnetic stirring bar It is 0.3cm and the length is 1.1cm. The silanization reaction time is 2h.

[0057] (2) Magnetic nanoparticles modified with terminal diaminobenzyl carboxyl groups (gf-Fe 3 o 4 ) preparation: 1g Fe 3 o 4 @SiO 2 Disperse nanoparticles in toluene, add 20mL silane coupling agent, rea...

Embodiment 3

[0061] (1) Preparation and activation of a magnetic stirring rod: Put a thin magnetic iron rod into a glass tube, sinter and seal both ends to make a magnetic stirring rod; Wash with water until neutral and activate; place the activated stirring bar in a silane coupling agent for silanization reaction, wash with methanol, and blow dry with nitrogen; the alkali solution is 1mol / L NaOH solution; the acid solution is 0.5mol / L L of hydrochloric acid solution; the soaking time is 18h and 2h respectively, and the silane coupling agent that reacts with the stirring bar is a γ-methacryloxypropyltrimethoxysilane acetone solution with a mass concentration of 20%; the diameter of the magnetic stirring bar It is 0.3cm and the length is 1.1cm. The silylation reaction time is 3h.

[0062] (2) Magnetic nanoparticles modified with terminal diaminobenzyl carboxyl groups (gf-Fe 3 o 4 ) preparation: 1g Fe 3 o 4 @SiO 2 Disperse nanoparticles in toluene, add 20mL silane coupling agent, react...

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Abstract

The invention discloses a preparation method of a clonazepam molecular imprinting stirring rod coating. The method comprises the following processes of preparation and activation of a magnetic stirring rod; preparation of magnetic nanoparticles modified by diaminobenzene carboxyl in the tail end; preparation of a magnetic field induced self-assemble molecular imprinting stirring rod. The out-field self-assemble molecular imprinting stirring rod prepared through the method provided by the invention has specific recognition capability on benzodiazepines compounds, compared with the traditional stirring rod, polymerization steps are reduced, the adsorption amount is increased, a molecular recognition mode with multiple functions is provided, and the adsorption efficiency in a polar solvent is obviously increased. The accuracy of the method when being applied to a practical sample test can be improved; the coating has no breakage or falling off phenomenon after being used repetitively, and is coupled with chromatography so as to be applied to separation and concentration of the benzodiazepines compounds in complex matrixes such as health food, feed and biological samples.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and specifically relates to a method for preparing a magnetic field-induced self-assembly clonazepam molecularly imprinted stirring rod coating. The prepared stirring rod coating has fast self-assembly, high specific selectivity, large adsorption capacity and Good practicality. Background technique [0002] In a tense and highly competitive society, lack of sleep or sleep disorders have seriously affected the lives of modern people. Benzodiazepines (BZ) are the most widely used drugs in the field of anti-anxiety and insomnia. Criminals often illegally add BZ to sedative and hypnotic traditional Chinese medicine health food, advertised as "pure traditional Chinese medicine preparation", and deceive consumers for huge profits. BZ has a series of known side effects such as drowsiness, drowsiness, and ataxia, such as dizziness, headache, excitement, restlessness, fatigue, slurred spe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/286B01D15/10
CPCB01D15/10B01J20/286
Inventor 周学敏李晓旭徐磊沈心朱婉莹
Owner NANJING MEDICAL UNIV
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