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Kit for detecting barrier function of intestinal tract

A kit and solvent technology, applied in the field of biological products, can solve the problems of difficult popularization, cumbersome operation, large trauma, etc., and achieve the effects of simple and fast operation, simple operation and high accuracy

Active Publication Date: 2016-06-22
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is cumbersome to operate, requires patients to take corresponding substances that are not easily absorbed, and requires corresponding testing instruments, so it is difficult to promote clinically.
Bacterial culture is commonly used in the diagnosis of bacterial translocation. Usually, mesenteric lymph nodes are taken under sterile conditions for bacterial culture. Bacteria are positive when cultured. However, this method is quite traumatic and difficult to carry out clinically.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Intestinal Barrier Function Detection Kit

[0046] This embodiment provides the preparation of the intestinal barrier function detection kit, the preparation method is as follows:

[0047] 1) Preparation of reaction substrate:

[0048] The reaction substrate is preferably, but not limited to, made of ABS engineering plastics, on which there are blank control wells, diamine oxidase reaction wells, D-lactic acid reaction wells and bacterial endotoxin reaction wells, and each reaction well is filled with the following The corresponding reaction pad described above; the size of the blank control well and the reaction well can be preferred

[0049] 2) Preparation of plasma separator:

[0050] The structure of the plasma separation membrane in the plasma separator can be conventionally used in the prior art, and the present invention does not specifically limit it. Whole blood is dripped on the plasma separation membrane to realize the separatio...

Embodiment 2

[0094] Example 2 The method of using the intestinal barrier function detection kit

[0095] This embodiment provides the method for using the intestinal barrier function detection kit, specifically:

[0096] After taking a small amount of whole blood from the fingertip with a blood collection needle, it is directly added to the plasma separator of the device. After the whole blood is separated by the plasma separation membrane, the serum reaches each reaction well of the device, and is in the reaction well of diamine oxidase and D-lactic acid. Add one drop (about 10 μL) of diluent, and add one drop (about 10 μL) of chromogenic solution into the bacterial endotoxin reaction well. Put the reaction device on top of the dry chemical detection instrument to react for 15 minutes. The concentration of the analyte can be obtained quantitatively through the quantitative interpretation of the dry chemical analyzer.

Embodiment 3

[0097] Example 3 Application of Intestinal Barrier Function Detection Kit

[0098] This kit provides a detection kit for the intestinal barrier function. It detects the blood samples of clinical patients to check whether the intestinal barrier function of the patient is impaired, so as to assist the patient in rebuilding the intestinal barrier function.

[0099] The specific usage of the kit is as follows:

[0100] 1) When in use, first take out the kit from 2°C to 8°C, equilibrate to room temperature and start using.

[0101] 2) Tear off the aluminum foil package and take out the reaction device.

[0102] 3) Use a blood collection tube to collect whole blood from the fingertip, and directly add it to the blood injection hole of each reaction well of the device; if the sample is serum or plasma, the plasma separation device needs to be torn off, and added to each reaction well 4 μL of sample is sufficient.

[0103] 4) Let it stand for a while and remove the plasma separator a...

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PUM

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Abstract

The invention provides a kit for detecting the barrier function of the intestinal tract. The kit comprises a reaction device, one or more colorimetric solutions and one or more dilution solutions; the reaction device comprises a blood plasma separator and a reaction substrate, and the reaction substrate includes a blank hole, a diamine oxidase reaction hole, a D-lactic acid reaction hole and a bacterial endotoxin reaction hole; a blood plasma separating film is arranged in each of the holes of the blood plasma separator; and a blank pad is arranged in the blank hole.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a kit for detecting intestinal barrier function, more specifically, to a kit for detecting D-lactic acid, diamine oxidase and bacterial endotoxin in blood. Background technique [0002] Intestinal barrier dysfunction is mainly manifested as intestinal mucosal barrier damage, intestinal microecological disorder and intestinal motility disorder. Intestinal barrier dysfunction is the damage and atrophy of intestinal mucosa caused by various reasons, the increase of intestinal permeability, and the imbalance of intestinal flora, which lead to the translocation of bacteria and (or) endotoxin, and can induce and (or) aggravate the systemic inflammatory response and multiple organ dysfunction. Intestinal barrier dysfunction has an important impact on the occurrence, development and outcome of critical diseases. Intestinal barrier dysfunction is common in critically ill pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52
Inventor 李维勤王加义桂春爽董婷婷
Owner 北京中生金域诊断技术股份有限公司
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