Preparation method of soybean peptide biological calcium
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A soybean peptide and bio-calcium technology, which is applied in food science, the function of food ingredients, and protein-containing food ingredients, etc., can solve the problems of small scope of action, low degree of hydrolysis, and no consideration of extraction, so as to improve human immunity, The effect of high content of available calcium
Active Publication Date: 2016-06-29
安徽中森生物技术有限公司
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However, this single enzyme has a small range of action and a low degree of hydrolysis
Chen Dan et al. used Lactobacillus bulgaricus to ferment ultra-finely pulverized yak bone mud, and obtained a high content of free calcium. However, this method only deals with calcium elements in product preparation, and does not take into account the protein, active peptides, amino acids, etc. in yak bones. Extraction of other beneficial substances to the human body, the function of the product is relatively simple
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Embodiment 1
[0021] A preparation method of soybean peptide biological calcium, comprising the following steps:
[0022] (1) Pass the substrate, yak bone powder and yak bone marrow powder through a 200-mesh sieve respectively, weigh 120 g of yak bone powder and 10 g of yak bone marrow powder, add 1300 mL of purified water, and then stir in a water bath with a rotating speed of 3000 r / min and a temperature of 40°C;
[0023] (2) Adjust the pH of the solution to 7, add neutral protease according to the ratio of 1600 activity units per gram of substrate, and then enzymatically hydrolyze for 45 minutes under water bath conditions, and the water bath temperature is 40°C;
[0024] (3) Adjust the pH of the solution again to 6, add papain at a ratio of 1600 activity units per gram of substrate, and enzymatically hydrolyze for 50 minutes under water bath conditions, with a water bath temperature of 60° C.;
[0025] (4) Add 43.3g soybean peptide to the solution in step (3), and then keep it warm at ...
Embodiment 2
[0029] A preparation method of soybean peptide biological calcium, comprising the following steps:
[0030] (1) Pass the substrate, yak bone powder and yak bone marrow powder through a 200-mesh sieve respectively, weigh 120 g of yak bone powder and 10 g of yak bone marrow powder, add 1300 mL of purified water, and then stir in a water bath with a rotating speed of 3000 r / min and a temperature of 40°C;
[0031] (2) Adjust the pH of the solution to 7, add neutral protease according to the ratio of 1600 activity units per gram of substrate, and then enzymatically hydrolyze for 45 minutes under water bath conditions, and the water bath temperature is 40°C;
[0032] (3) Adjust the pH of the solution again to 6, add papain at a ratio of 1600 activity units per gram of substrate, and enzymatically hydrolyze for 50 minutes under water bath conditions, with a water bath temperature of 60° C.;
[0033] (4) Add 65g soybean peptide to the solution in step (3), and then keep it warm at 40...
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Abstract
The invention discloses a preparation method of soybean peptide biological calcium. The soybean peptide biological calcium is prepared through the following steps of using substrate yak bone powder and yak bone marrow powder as natural calcium sources, adjusting the pH, controlling the temperature of a water bath, and under the action of enzymes, enabling soybean peptides and the natural calcium sources in the water bath to be made into soybean peptide biological calcium products. The soybean peptide biological calcium prepared by the method disclosed by the invention is high in content of effective calcium. According to the preparation method, insoluble hydroxyl calcium phosphate in yak bones is effectively converted into the soluble soybean peptide biological calcium. Each 100g of the yak bone powder contains 22g of the calcium, and the extraction rate of calcium components in the yak bone powder can achieve 27%. The prepared soybean peptide biological calcium also reserves other substances, such as protein, bioactive peptides and amino acids in the yak bones and bone marrows, which are beneficial for human bodies. The yak bone marrow powder is added, so that the conversion rate of the soluble calcium components is increased, and besides, the beneficial substances such as the protein and the macromolecule peptides are converted into micromolecular polypeptide substances through enzymolysis, and are completely reserved. The soybean peptide biological calcium has the effect of improving the immunity of human bodies.
Description
technical field [0001] The invention relates to the field of health food, in particular to a preparation method of soybean peptide biological calcium. Background technique [0002] Calcium is an essential constant element for the human body. Calcium deficiency affects the normal growth and development of children, causing rickets, and middle-aged and elderly people are prone to osteoporosis and osteomalacia. In order to increase the absorption of calcium in the human body, a comparative study was carried out on the supplementary effects of various inorganic and organic calcium, and it was found that compounds such as amino acids and peptides have a good ability to promote calcium absorption. Many in vivo and in vitro experiments have confirmed that casein phosphopeptide (CPP) combines the phosphate group of phosphoserine with calcium ions in the lower small intestine to form a soluble complex to improve the calcium absorption rate of the human body. At present, studies have...
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